Novel NP and RP-HPTLC in Praxis for Simultaneous Estimation of Chlorthalidone and Cilnidipine in Bulk and Pharmaceutical Formulation

Rathod, Ravsaheb H.; Patil, Amod S.; Shirkhedkar, Atul A.

doi:10.1080/22297928.2018.1527252

Cited by 16 publications

(4 citation statements)

References 5 publications

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“…Determination of BF alone and in combinations with other drugs is reported in the literature by various methods, that is, UV Spectrophotometry, [6][7][8][9] HPTLC, [10][11][12] HPLC, [13][14][15][16][17][18][19] and capillary electrophoresis. [20][21][22] Furthermore, determination of CLD alone or with other drugs by HPTLC, [23][24][25][26][27][28] HPLC, [29][30][31][32][33][34] and spectrophotometry [30,[35][36][37][38][39][40][41][42][43][44][45] is reported. The literature survey reveals only two reported spectrophotometric methods for simultaneously determining BF and CLDusly.…”

Section: Original Articlementioning

confidence: 99%

Untitled

2022

AJP

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Introduction: Cardiovascular disorders involve simultaneous administration of more than one drug; hence, numerous combinations, including calcium channel blockers and beta-blockers, are available in the market and are approved by various regulating authorities. Cilnidipine (CLD), a calcium-channel blocker, and Bisoprolol fumarate, a β1-adrenergic receptor blocking agent are such combinations available on the market. This combination is used in the treatment of hypertension and angina pectoris. Therefore, these drugs must be accurately analyzed in various samples, including laboratory mixtures and marketed formulations. Methods: Simultaneous estimation of Bisoprolol fumarate and CLD in marketed formulation Basicore C5 was carried out using the UV spectrophotometric method in ethanol. The Q-Absorbance ratio method was selected for estimation. The wavelengths used were 224.5 nm, λmax of Bisoprolol fumarate, and 232 nm, an iso-absorptive point of both the drugs. Results: Beer's law was obeyed in the concentration range between 2 and 10 μg/mL for both the drugs. Recovery studies have validated the analysis results as per ICH guidelines. Accuracy was found between 99.3-99.75% and 99.48-100.06% for Bisoprolol fumarate and CLD, respectively. The limit of detection was 0.0943 and 0.0751 μg/mL for Bisoprolol fumarate, while 0.0355 and 0.1141 μg/mL for CLD at 224.5 and 232 nm, respectively. The limit of quantifications was 0.2860 and 0.8983 μg/mL for Bisoprolol fumarate, while 0.1610 and 0.3457 μg/mL for CLD at 224.5 and 232 nm, respectively. Conclusion: The method was simple, reproducible, rapid, and precise. Hence, it could analyze laboratory samples and marketed formulations containing these two drugs.

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Section: Original Articlementioning

confidence: 99%

Untitled

2022

AJP

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“…The chemical structures of AZL and CIL are shown in Figure 1. Numerous RP-HPLC methods have been published in the literature for the estimation of AZL alone and with other drugs [12][13][14][15][16][17][18][19][20], CIL alone, and with other drugs [21][22][23][24][25] in their FDCs. Recently, some chromatographic methods such as RP-HPLC and ultra-performance liquid chromatography methods have been reported in the literature for simultaneous estimation of AZL and CIL using class 2 solvents (methanol and acetonitrile) as mobile phase and diluent [26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Robust method operable design region for economical and eco‐friendly chromatographic analysis of azilsartan medoxomil and cilnidipine by incorporating a hybrid approach of green analytical chemistry and analytical quality by design

Prajapati,

Shahi,

Acharya

et al. 2023

Separation Science Plus

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According to the concept of green analytical chemistry, the analytical method development should be carried out by avoiding or minimizing the usage of toxic organic solvents for the safety of human and aquatic animal life and the protection of the environment. Hence, the green analytical chemistry (GAC)‐assisted robust liquid chromatographic method has been developed for chromatographic analysis of azilsartan medoxomil and cilnidipine (CIL) using safe organic solvents. The chromatographic method was developed by the implementation of analytical quality by design using chemometrics and response surface modeling. The chromatographic separation was carried out using Shim‐Pack C18 (250 × 4.6 mm, 5.0 μm) column as stationary phase and ethanol: 0.1% V/V ammonia solution (45:55, %V/V) as mobile phase. The chromatographic peak of AZL and CIL was found to be at the retention time of 3.5 and 4.5 min, respectively. The flow rate was kept at 1.0 mL/min and the column oven temperature was set to 40˚C. The developed method was found to be validated as per the International Council for Harmonization Q2 (R1) guideline. The method was applied for the assay of fixed‐dose combination and results were found in compliance with the labeled claim. The greenness of the method was evaluated using GAC tools.

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“…CLN has a relatively rapid absorption with a peak concentration after 2 h [11]. CLN is an antihypertensive, vasodilator dual blocker of L-type voltage-gated Ca2+channels in vascular smooth muscle and N-type Ca2+channels in sympathetic nerve terminals that supply the blood vessels [12,13]. Because there are so many distinct drug compounds, it is vital to ensure their safety before these treatments are marketed.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of the Rp-HPLC Method for Estimation of Cilnidipine in Rat Plasma

Jaiswal

WADETWAR²

2022

Int J Pharm Pharm Sci

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Objective: A simple, precise, accurate, robust, and reliable RP-HPLC technique for detecting cilnidipine in Wistar rat plasma was developed and validated. Methods: The chromatographic separation was performed using the C-18 reverse phase column Oyster BDS Premium C18 column (4.6 × 150 mm, 5 µm particle size). The flow rate was set to 1.2 ml/min, and the chromatogram was recorded at 240 nm. The proposed method was validated in compliance with the International Conference on Harmonization (ICH) guidelines. Results: The calibration curves plotted were found to be linear over the concentration range of 40-200 ng/ml in Wistar rat plasma, with a regression coefficient of R2>0.999, suggesting that the linearity was within limits. The LOD and LOQ of the present method were found to be 2.184 and 4.518 ng/ml, respectively. Drug extraction recoveries from Wistar rat plasma were found to be >98%. Conclusion: Using Wistar rat plasma, a simple, fast, specific, accurate, and precise analytical approach was developed and validated. The technique was validated according to the ICH guidelines. The obtained results show that the suggested technique can be simply and effectively utilized for routine cilnidipine analysis in Wistar rat plasma.

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Novel NP and RP-HPTLC in Praxis for Simultaneous Estimation of Chlorthalidone and Cilnidipine in Bulk and Pharmaceutical Formulation

Cited by 16 publications

References 5 publications

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Robust method operable design region for economical and eco‐friendly chromatographic analysis of azilsartan medoxomil and cilnidipine by incorporating a hybrid approach of green analytical chemistry and analytical quality by design

Development and Validation of the Rp-HPLC Method for Estimation of Cilnidipine in Rat Plasma

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