Novel NSP1 genotype characterised in an African camel G8P[11] rotavirus strain

Jere, Khuzwayo C.; Esona, Mathew D.; Ali, Yahia Hassan; Peenze, Ina; Roy, Sunando; Bowen, Michael D.; Saeed, Intisar Kamil; Khalafalla, Abdelmalik I.; Nyaga, Martin M.; Mphahlele, M. Jeffrey; Steele, A. Duncan; Seheri, Mapaseka

doi:10.1016/j.meegid.2013.10.002

Cited by 35 publications

(31 citation statements)

References 28 publications

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“…RVA genomic classification studies have revealed the emergence of novel or unusual RVAs carrying several novel gene segments or genotype constellations (Abe et al, 2011; Esona et al, 2010; Jere et al, 2014; Matthijnssens et al, 2011; Papp et al, 2012; Trojnar et al, 2013; Yinda et al, 2016). In this study, we identified and characterized a RVA strain with an unusual genotype constellation and carrying eight recently assigned novel gene segments.…”

Section: Resultsmentioning

confidence: 99%

“…The Rotavirus Classification Working Group (RCWG) has devised a complete genotype classification system, defining the genotype constellation of RVAs as follows: Gx-P[ x ]-Ix-Rx-Cx-Mx-Ax-Nx-Tx-Ex-Hx, representing VP7-VP4-VP6-VP1-VP2-VP3-NSP1-NSP2-NSP3-NSP4-NSP5 (Matthijnssens et al, 2011). To date, 35 G, 50 P, 26 I, 21 R, 19C, 19 M, 30 A, 20 N, 21 T, 26 E and 21H genotypes have been identified in human and non-human hosts and classified based on differences in the nucleotide sequence identities of each encoding gene segment, respectively (Abe et al, 2011; Esona et al, 2010; Estes and Greenberg, 2013; Guo et al, 2012; Jere et al, 2014; Matthijnssens et al, 2011; Papp et al, 2012; Trojnar et al, 2013; Yinda et al, 2016), http://rega.kuleuven.be/cev/viralmetagenomics/virus-classification). This classification system has accelerated the comparison of RVA genotypes and increased our understanding of the genetic diversity of RVA.…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of a human G20P[28] rotavirus a strain with multiple genes related to bat rotaviruses

Esona

Roy

Rungsrisuriyachai

et al. 2018

Infection, Genetics and Evolution

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Group A rotaviruses are the major cause of severe gastroenteritis in the young of mammals and birds. This report describes characterization of an unusual G20P[28] rotavirus strain detected in a 24 month old child from Suriname. Genomic sequence analyses revealed that the genotype constellation of the Suriname strain RVA/Human-wt/SUR/2014735512/2013/G20P[28] was G20-P[28]-I13-R13-C13-M12-A23-N13-T15-E20-H15. Genes VP1, VP2, VP3, NSP1, NSP2, NSP3, NSP4 and NSP5 were recently assigned novel genotypes by the Rotavirus Classification Working Group (RCWG). Three of the 11 gene segments (VP7, VP4, VP6) were similar to cognate gene sequences of bat-like human rotavirus strain Ecu534 from Ecuador and the VP7, NSP3 and NSP5 gene segments of strain RVA/Human-wt/SUR/2014735512/2013/G20P[28] were found to be closely related to gene sequences of bat rotavirus strain 3081/BRA detected in Brazil. Although distantly related, the VP1 gene of the study strain and bat strain BatLi09 detected in Cameroon in 2014 are monophyletic. The NSP1 gene was found to be most closely related to human strain QUI-35-F5 from Brazil. These findings suggest that strain RVA/Human-wt/SUR/2014735512/2013/G20P[28] represents a zoonotic infection from a bat host.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular characterization of a human G20P[28] rotavirus a strain with multiple genes related to bat rotaviruses

Esona

Roy

Rungsrisuriyachai

et al. 2018

Infection, Genetics and Evolution

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“…While GH018-08 was closely related (96.1% nucleotide sequence identity) to the human-artiodactyl interspecies reassortant strain RVA/Human-wt/BEL/B1711/2002/G6P[6] [49], its next closest neighbour (91.8% nucleotide sequence identity) was the caprine strain RVA/Goat-tc/BGD/GO34/1999/G6P[1] [50]. GH018-08 was distantly related (90.2–91.6% nucleotide sequence identities) to other contemporary human strains of the same genotype, as well as the recently reported camel strain RVA/Camel-wt/SDN/MRC-DPRU447/2002/G8P[11] [51] (90.8% nucleotide sequence identity) (Figure 2, Tables S3, S4). However, GH019-08 was closely related (99.1–99.2% nucleotide sequence identity) to the contemporary human strains RVA/Human-wt/BEL/F01498/2009/G3P6, RVA/Human-wt/BEL/F01322/2009/G3P[6] and RVA/Human-wt/USA/06-242/2006/G2P[6] [52], as well as other recent human strains (97.3–98.2% nucleotide sequence identity), including many of African origin.…”

Section: Resultsmentioning

confidence: 99%

Identification of Novel Ghanaian G8P[6] Human-Bovine Reassortant Rotavirus Strain by Next Generation Sequencing

Dennis

Fujii²,

Haga³

et al. 2014

PLoS ONE

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Group A rotaviruses (RVAs) are the most important etiological agent of acute gastroenteritis in children <5 years of age worldwide. The monovalent rotavirus vaccine Rotarix was introduced into the national Expanded Programme on Immunization (EPI) in Ghana in May 2012. However, there is a paucity of genetic and phylogenetic data on the complete genomes of human RVAs in circulation pre-vaccine introduction. The common bovine rotavirus VP7 genotype G8 has been sporadically detected in Ghanaian children, usually in combination with the VP4 genotype P[6]. To investigate the genomic constellations and phylogeny of RVA strains in circulation prior to vaccine introduction, the full genomes of two unusual G8P[6] strains, GH018-08 and GH019-08, detected during burden of disease surveillance, were characterized by Illumina MiSeq sequencing. The Ghanaian isolates, GH018-08 and GH019-08, exhibited the unusual, previously unreported genotype constellation G8-P[6]-I2-R2-C2-M2-A2-N2-T2-E2-H3. Phylogenetic analyses confirmed that 10 out of the 11 genes of GH018-08 and GH019-08 were identical/nearly identical, with significant variation detected only in their VP1 genes, and clearly established the occurrence of multiple independent interspecies transmission and reassortment events between co-circulating bovine/ovine/caprine rotaviruses and human DS-1-like RVA strains. These findings highlight the contribution of reassortment and interspecies transmission events to the high rotavirus diversity in this region of Africa, and justify the need for simultaneous monitoring of animal and human rotavirus strains.

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“…The NSP3 genes of strains HMG035 and NGRBg8 showed complete nucleotide sequence identity (100%), and the maximum nucleotide sequence identity (98.3%) with the NSP3 gene of Ghanaian bovine-like human strain Ghan-059 (G8P[1]), and somewhat lower identity (95.5%) with Italian bovine-like human strain PA169 (G6P[14]) (Matthijnssens et al, 2008a). On phylogenetic analysis, strains HMG035 and NGRBg8 were found to be closely related with strain Ghan-059 in a common branch with strain PA169 and Sudanese bovine-like camel strain MRC-DPRU447 (G8P[11]) (Jere et al, 2014) (Fig. 1i).…”

Section: Phylogenetic Analysesmentioning

confidence: 99%

Whole genomic analysis of human and bovine G8P[1] rotavirus strains isolated in Nigeria provides evidence for direct bovine-to-human interspecies transmission

Komoto

Adah

Ide

et al. 2016

Infection, Genetics and Evolution

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Novel NSP1 genotype characterised in an African camel G8P[11] rotavirus strain

Cited by 35 publications

References 28 publications

Molecular characterization of a human G20P[28] rotavirus a strain with multiple genes related to bat rotaviruses

Molecular characterization of a human G20P[28] rotavirus a strain with multiple genes related to bat rotaviruses

Identification of Novel Ghanaian G8P[6] Human-Bovine Reassortant Rotavirus Strain by Next Generation Sequencing

Whole genomic analysis of human and bovine G8P[1] rotavirus strains isolated in Nigeria provides evidence for direct bovine-to-human interspecies transmission

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