Novel Object Recognition in the Rat: A Facile Assay for Cognitive Function

Mathiasen, Joanne R.; DiCamillo, Amy

doi:10.1002/0471141755.ph0559s49

Cited by 91 publications

(57 citation statements)

References 40 publications

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“…Tests were conducted during two periods: the 1st day of treatment and prior to euthanasia. The recognition index (RI) was calculated: RI = TN/(TN + TF) × 100, where TN = time spent with novel object and TF = time spent with familiar object [14,15].…”

Section: Novel Object Recognitionmentioning

confidence: 99%

Behavioural changes observed in demyelination model shares similarities with white matter abnormalities in humans

Serra-de-Oliveira

Boilesen

Carvalho

et al. 2015

Behavioural Brain Research

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Section: Novel Object Recognitionmentioning

confidence: 99%

Behavioural changes observed in demyelination model shares similarities with white matter abnormalities in humans

Serra-de-Oliveira

Boilesen

Carvalho

et al. 2015

Behavioural Brain Research

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“…This test provides information about cognitive function and also about the animal's lateralization . The animals were tested in the same open area used for open field testing (45.5 × 45.5 cm), but this time the walls were transparent and the behavior was videotracked by AnyMaze software (Stoelting, Wood Dale, IL, U.S.A.).…”

Section: Methodsmentioning

confidence: 99%

Estradiol does not affect spasms in the betamethasone‐NMDA rat model of infantile spasms

et al. 2016

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SUMMARYObjective: This study attempted to validate the effects of neonatal estradiol in ameliorating the spasms in the prenatally betamethasone-primed N-methyl-D-aspartate (NMDA) model of infantile spasms in rats as shown previously in a mouse Arx gene knock-in expansion model of infantile spasms. Methods: Neonatal rats prenatally exposed to betamethasone (on day 15 of pregnancy) were treated with subcutaneous 40 ng/g estradiol benzoate (EB) between postnatal days (P)3-P10 or P0-P5. A synthetic estrogen analogue, diethylstilbestrol, was used between P0 and P5 (2 lg per rat, s.c.). On P12, P13, and P15, the rats were subjected to NMDA-triggered spasms, and latency to onset and number of spasms were evaluated. Rats with EB on P3-P10 were tested after spasms in the open field, novel object recognition, and elevated plus maze to determine effects of treatment on behavior. Additional rats with P3-P10 or P0-P5 EB were investigated for c-aminobutyric acid (GABA)ergic neurons (glutamate decarboxylase [GAD]67 expression) in the neocortex. As a positive control, a group of rats received either subcutaneous adrenocorticotropic hormone (ACTH) (2 3 0.3 mg/kg on P12 and 3 3 0.3 mg/kg on P13 and P14) or vehicle after the first episode of spasms on P12. Results: Neither EB treatment nor diethylstilbestrol consistently affected expression of spasms in this model, although we found a significant increase in GAD67-immunopositive cells in the neocortex after P3-P10 and P0-P5 EB treatment, consistent with a study in mice. Behavioral tests showed increase in lateralization in male rats treated with P3-P10 EB, a behavioral trait usually associated with female sex. Diethylstilbestrol treatment in male rats resulted in arrested pubertal descent of testes. ACTH had robust effects in suppressing spasms. Significance: Treatment of infantile spasms (IS) using neonatal EB may be justified in those cases of IS that present with detectable deficits in GABAergic neurons. In other types of IS, the efficacy of neonatal EB and its analogues is not supported.

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“…Exploration was defined as directing the nose to the object at a distance of no more than 2 cm and/or touching the object with the nose or mouth. Rearing up on the object was counted only if facing toward, but not away from, the object (33,34). …”

Section: Methodsmentioning

confidence: 99%

Effects of Early Chemotherapeutic Treatment on Learning in Adolescent Mice: Implications for Cognitive Impairment and Remediation in Childhood Cancer Survivors

Bisen-Hersh

Hineline

Walker

2013

Clinical Cancer Research

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Purpose Among children diagnosed with acute lymphoblastic leukemia (ALL) and given chemotherapy-only treatment, 40-70% of survivors experience neurocognitive impairment. The present study used a preclinical mouse model to investigate the effects of early exposure to common ALL chemotherapeutics methotrexate (MTX) and cytarabine (Ara-C) on learning and memory. Experimental Design Pre-weanling mouse pups were treated on postnatal day (PND) 14, 15, and 16 with saline, MTX, Ara-C, or a combination of MTX and Ara-C. Nineteen days following treatment (PND 35), behavioral tasks measuring different aspects of learning and memory were administered. Results Significant impairment in acquisition and retention over both short (1h) and long (24h) intervals, as measured by autoshaping and novel object recognition tasks, were found following treatment with MTX and Ara-C. Similarly, a novel conditional discrimination task revealed impairment in acquisition for chemotherapy-treated mice. No significant group differences were found following the extensive training component of this task, with impairment following the rapid training component occurring only for the highest MTX and Ara-C combination group. Conclusions Findings are consistent with clinical studies suggesting that childhood cancer survivors are slower at learning new information and primarily exhibit deficits in memory years after successful completion of chemotherapy treatment. The occurrence of mild deficits on a novel conditional discrimination task suggests that chemotherapy-induced cognitive impairment may be ameliorated through extensive training or practice.

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Novel Object Recognition in the Rat: A Facile Assay for Cognitive Function

Cited by 91 publications

References 40 publications

Behavioural changes observed in demyelination model shares similarities with white matter abnormalities in humans

Behavioural changes observed in demyelination model shares similarities with white matter abnormalities in humans

Estradiol does not affect spasms in the betamethasone‐NMDA rat model of infantile spasms

Effects of Early Chemotherapeutic Treatment on Learning in Adolescent Mice: Implications for Cognitive Impairment and Remediation in Childhood Cancer Survivors

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