Novel optimized biofunctional surfaces for Love mode surface acoustic wave based immunosensors

Dinh, Duy Hai; Pascal, Emilie; Vellutini, Luc; Bennetau, Bernard; Rebière, Dominique; Déjous, Corinne; Moynet, Daniel; Belin, Claude; Pillot, Jean‐Paul

doi:10.1016/j.snb.2010.02.008

Cited by 16 publications

(10 citation statements)

References 51 publications

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“…Generally, the commercially available (3-glycidoxypropyl)trimethoxysilane (GPTS) is used to functionalize the silica surfaces for the chemical immobilization of small molecules, − polymers, − or biomolecules. , However, in the case of non-atomically flat substrates, this short-chain arm can adopt various orientations and some epoxide groups may not be accessible to the species to immobilize. Thus, in a recent study, we have shown that a very weak sensor response was measured when GPTS SAM was used to immobilize Escherichia coli bacteria in liquid medium onto relatively rough silica surfaces [root-mean-square (rms) roughness ≈ 5.5 nm] . In contrast, a significant sensor response was measured when a very long-chain (C 22 ) glycidyl-terminated SAM was used to immobilize bacteria on the surface.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, in a recent study, we have shown that a very weak sensor response was measured when GPTS SAM was used to immobilize Escherichia coli bacteria in liquid medium onto relatively rough silica surfaces [root-meansquare (rms) roughness ≈ 5.5 nm]. 21 In contrast, a significant sensor response was measured when a very long-chain (C 22 ) glycidyl-terminated SAM was used to immobilize bacteria on the surface. This improvement was attributed to a marked surface smoothing, leading to a better efficiency of antigen− antibody recognition processes at the surface.…”

Section: Introductionmentioning

confidence: 99%

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Epoxy-Terminated Self-Assembled Monolayers Containing Internal Urea or Amide Groups

et al. 2015

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We report the synthesis of new coupling agents with internal amide or urea groups possessing an epoxy-terminal group and trimethoxysilyl-anchoring group. The structural characterizations of the corresponding self-assembled monolayers (SAMs) were performed by polarization modulation infrared reflection adsorption spectroscopy (PM-IRRAS). The molecular assembly is mainly based on the intermolecular hydrogen-bonding between adjacent amide or urea groups in the monolayers. Because of the steric hindrance of amide or urea groups, the distance between the alkyl chains is too large to establish van der Waals interactions, inducing their disorder. The reactivity of the epoxy-terminal groups was successfully investigated through reaction with a fluorescent probe. We show that SAMs containing internal urea or amide groups exhibited a higher density of accessible epoxide groups than the corresponding long-chain (C22) glycidyl-terminated SAM.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Epoxy-Terminated Self-Assembled Monolayers Containing Internal Urea or Amide Groups

et al. 2015

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“…In addition, adhesion of the hybrid layer on the quartz substrates was much stronger than in the case of silica PECVD deposition, where partial splitting off could not be prevented during preparation of samples for SEM study. 57 These results also showed that the cleaning method of the quartz substrate which was used in this work (UV/ozone) was very efficient, 58 affording a good compatibility between the substrate and the hybrid layer deposit. This method generates hydroxyl groups on the quartz surface, which is crucial for the covalent attachment of BTEBP molecules via Si-O-Si bonds.…”

Section: Hybrid Film Synthesismentioning

confidence: 63%

Chimie douce route to novel acoustic waveguides based on biphenylene-bridged silsesquioxanes

et al. 2011

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International audienceNovel silsesquioxane thin films were conveniently synthesized by the sol-gel route under mild conditions. Thus, a sol was prepared from 4,4′-bis(triethoxysilyl)-1,1′-biphenyl (4,4′-BTEBp) under a hydroalcoholic medium without a surfactant, deposited by spin-coating on silica supports, and dried at 280 °C. The resulting biphenylene-bridged films exhibited very smooth surfaces, excellent mechanical behavior and no cracks. Moreover, SEM and TEM studies showed flawless, homogeneous interfaces with silica substrates. The acoustic waveguide properties of these films on piezoelectric quartz microsensors were investigated at high frequency through a network analyzer. For an optimal thickness close to 1 μm, a marked frequency shift of the main broad lobe and a decrease of the insertion loss were observed. These results show a strong effect of the waveguide for trapping the acoustic energy, promising an unprecedented ability to provide a new generation of sensors. The capability of this device in the detection of mass is also demonstrated through the immobilization of Bovine Serum Albumin (BSA). This novel approach appears very promising in view of the development of surface acoustic wave sensors and biosensors

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“…The pathogenic bacteria Vibrio harveyi , which causes morbidity in commercial shrimp cultures, was detected using an antibody that showed no detectable cross reactivity to Vibrio vulnificus nor Vibrio parahaemolyticus (Buchatip et al ., ). Live Escherichia coli were detected using antibodies immobilised on a long chain organosilicon self assembled monolayer, with the nature of the monolayer being important for successful detection (Dinh et al ., ). Measurement of bacterial growth in liquid culture was also achieved in a designed static liquid cell with a QCM sensor (Swiatkowski et al ., ).…”

Section: Bacteriamentioning

confidence: 97%

A Survey of the 2010 Quartz Crystal Microbalance Literature

Speight

Cooper

2012

J of Molecular Recognition

135

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In 2010 there has again been an increase in the number of papers published involving piezoelectric acoustic sensors, or quartz crystal microbalances (QCM), when compared to the last period reviewed 2006-2009. The average number of QCM publications per annum was 124 in the period 2001-2005, 223 in the period 2006-9, and 273 in 2010. There are trends towards increasing use of QCM in the study of protein adsorption to surfaces (93% increase), homeostasis (67% increase), protein-protein interactions (40% increase), and carbohydrates (43% increase). New commercial systems have been released that are driving the uptake of the technology for characterisation of binding specificities, affinities, kinetics and conformational changes associated with a molecular recognition event. This article highlights theoretical and practical aspects of the principals that underpin acoustic analysis, then reviews exemplary papers in key application areas involving small molecular weight ligands, carbohydrates, proteins, nucleic acids, viruses, bacteria, cells, and membrane interfaces.

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Novel optimized biofunctional surfaces for Love mode surface acoustic wave based immunosensors

Cited by 16 publications

References 51 publications

Epoxy-Terminated Self-Assembled Monolayers Containing Internal Urea or Amide Groups

Epoxy-Terminated Self-Assembled Monolayers Containing Internal Urea or Amide Groups

Chimie douce route to novel acoustic waveguides based on biphenylene-bridged silsesquioxanes

A Survey of the 2010 Quartz Crystal Microbalance Literature

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