Novel P-Solubilizers from Calcium Bound Phosphate Rich Pine Forest of Lower Himalaya

Nazir, Rashid; Hayat, Waseem; Rehman, Palwasha; Iqbal, Akhtar; Irshad, Usman

doi:10.1080/01490451.2016.1149256

Cited by 9 publications

(7 citation statements)

References 18 publications

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“…This medium was used because of its specificity for the isolation of efficient phosphorus solubilizing bacteria ( Nautiyal, 1999 ) and because TCP it is most abundant form of P present in alkaline soils where solution P immediately converts into fixed P by binding with calcium. The concentration of TCP was adjusted according to previous reports ( Irshad et al, 2011 ; Nazir et al, 2017 ). Plates were incubated for 48 h at 30°C and 20 bacterial colonies were able to solubilize TCP.…”

Section: Methodsmentioning

confidence: 99%

“…Their ability to grow and to liberate free phosphate in liquid Pikovskaya’s medium with TCP (same composition as above without agar) was further studied. The identification of the isolates was carried out as described by Nazir et al (2017) , using purified bacterial DNA as a template for PCR product targeting the 16S rRNA gene using primers 27F (5-AGAGTTTGATCCTGGCTCAG-3) and 1492R (5GGTTACCTTGTTACGACTT-3). PCR products were purified by using a QIAquick PCR purification kit and sent for Sanger sequencing at the McGill University nd Genome Quebec Innovation Center.…”

Section: Methodsmentioning

confidence: 99%

“…Even when the phosphorus inputs are very high, less than 5% of the soil P is bioavailable to plants ( Brady and Weil, 2008 ). Since up to 40% of cultivable microbes can solubilize inorganic P and release plant utilizable forms directly into solution ( Nazir et al, 2017 ), efforts to improve efficiency of P use are focused on microorganisms. Many P-solubilizing bacteria are found in the Pseudomonas and Bacillus genera ( Illmer and Schinner, 1992 ; Irshad et al, 2011 ), and they generally mineralize/solubilize P either by secreting organic acids or phosphatases ( Gugi et al, 1991 ; Lemanowicz, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

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Bacterial Subspecies Variation and Nematode Grazing Change P Dynamics in the Wheat Rhizosphere

Irshad

Yergeau

2018

Front. Microbiol.

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Low phosphorus soils are thought to constitute the majority of soils worldwide and cannot support intensive agriculture without high fertilizer inputs. Rhizobacteria are well-known to modify P dynamics and an increased bacterial diversity normally has a positive impact on various process rates. However, it is not known how variation in bacterial diversity at the subspecies level could influence trophic interactions in the rhizosphere and its consequences on plant P nutrition. We therefore hypothesized that the interactions between closely related P solubilizing bacteria and their grazing nematodes could improve plant P dynamics from an unavailable P source. We isolated four Pseudomonas poae strains and extracted nematodes from a Saskatchewan wheat field soil sample. The potential of all bacterial isolates with and without nematodes for increasing P availability in the wheat rhizosphere was tested in controlled microcosms with Ca3(PO4)2 as sole P source. Liberated P, phosphatase activity, plant P and bacterial abundance based on phnX gene copies were determined. Phosphorus solubilization efficiency of isolates varied between isolates whereas phosphatase enzyme activity was only detected under nematodes grazing and during the first 15 days of the experiment. Nematodes grazing upon individual Pseudomonas poae increased phosphatase enzyme activity, bacterial abundance, but decreased plant P concentration compared to non-grazed system. In contrast, the treatment combining all Pseudomonas poae isolates together with nematodes resulted in significant increases in P availability and plant P concentration. Diverse P-solubilizing efficiency and interaction with nematodes within the same bacterial “species” suggest that P dynamics might be linked to micro variation in soil diversity that would not accurately be picked up using common tools such as 16S rRNA gene sequencing.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bacterial Subspecies Variation and Nematode Grazing Change P Dynamics in the Wheat Rhizosphere

Irshad

Yergeau

2018

Front. Microbiol.

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“…The fungi often perform key steps in the breakdown of complex organic materials, yielding small molecules which are then further decomposed by bacteria that occur in the same habitat. The latter may contribute to nutrient provision to plants as well, e.g., performing important steps of the nitrogen cycle, such as nitrogen fixation ( Nelson and Sadowsky, 2015 ) or phosphate solubilization ( Nazir et al, 2016 ). Due to their mycelial way of growth, fungi can form true networks of interconnected hyphae in the soil as well as in the mycorrhizosphere and endosphere.…”

Section: Introductionmentioning

confidence: 99%

The Ecological Role of Type Three Secretion Systems in the Interaction of Bacteria with Fungi in Soil and Related Habitats Is Diverse and Context-Dependent

et al. 2017

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Bacteria and fungi constitute important organisms in many ecosystems, in particular terrestrial ones. Both organismal groups contribute significantly to biogeochemical cycling processes. Ecological theory postulates that bacteria capable of receiving benefits from host fungi are likely to evolve efficient association strategies. The purpose of this review is to examine the mechanisms that underpin the bacterial interactions with fungi in soil and other systems, with special focus on the type III secretion system (T3SS). Starting with a brief description of the versatility of the T3SS as an interaction system with diverse eukaryotic hosts, we subsequently examine the recent advances made in our understanding of its contribution to interactions with soil fungi. The analysis used data sets ranging from circumstantial evidence to geneknockout-based experimental data. The initial finding that the abundance of T3SSs in microbiomes is often enhanced in fungal-affected habitats like the mycosphere and the mycorrhizosphere is now substantiated with in-depth knowledge of the specific systems involved. Different fungal-interactive bacteria, in positive or negative associations with partner fungi, harbor and express T3SSs, with different ecological outcomes. In some particular cases, bacterial T3SSs have been shown to modulate the physiology of its fungal partner, affecting its ecological characteristics and consequently shaping its own habitat. Overall, the analyses of the collective data set revealed that diverse T3SSs have assumed diverse roles in the interactions of bacteria with host fungi, as driven by ecological and evolutionary niche requirements.

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“…Two internal primers (785F and 907R) were also used to obtain basic processing and reliable sequencing. [ 59 ]…”

Section: Methodsmentioning

confidence: 99%

The Fate of Lead (Pb) in Multitrophic Interactions Among Bacteria, Fungi, and Bacterivorous Soil Nematodes

Iqbal

Ahmed

Pervez

et al. 2020

CLEAN Soil Air Water

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Contamination of potentially toxic metals poses threats to living organisms due to prolonged existence in an ecosystem. The effects of toxic metals on soil microbes have broadly been reported but the study on interactions among soil micro‐ and macro‐organisms under metal stress needs to be elaborated. In current study, the immobilization of lead (Pb) is investigated by using five resistant microbial strains; (2) Bacillus cereus, (1) Bacillus subtilis, (1) Brevundimonas naejangsanensis, and (1) fungal strain Fusarium equiseti isolated from contaminated soil of Hattar, Pakistan. Bacterial and fungal strains and their consortia are tested in the presence/absence of nematodes for Pb immobilization in a medium containing 250 mg L−1 Pb. Single bacterial strains immobilize Pb up to 72%, while in the presence of nematodes, bacterial immobilization potential decreases. An increased Pb immobilization up to 185 mg L−1 is found when bacterial and fungal consortia are inoculated with nematodes. The maximum 80% Pb immobilization is found in bacterial–bacterial combinations with nematodes. This study, reveals that inoculation of bacterial–bacterial consortia in the presence of nematodes enhanced bacterial growth via feeding resulting in higher Pb immobilization. These resistant microbial strains can thus be used to treat contaminated sites for better ecosystem functioning.

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Novel P-Solubilizers from Calcium Bound Phosphate Rich Pine Forest of Lower Himalaya

Cited by 9 publications

References 18 publications

Bacterial Subspecies Variation and Nematode Grazing Change P Dynamics in the Wheat Rhizosphere

Bacterial Subspecies Variation and Nematode Grazing Change P Dynamics in the Wheat Rhizosphere

The Ecological Role of Type Three Secretion Systems in the Interaction of Bacteria with Fungi in Soil and Related Habitats Is Diverse and Context-Dependent

The Fate of Lead (Pb) in Multitrophic Interactions Among Bacteria, Fungi, and Bacterivorous Soil Nematodes

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