“…Cell counting, harvesting and permeabilization were performed as in Wilfinger et al ( 2016 ), with only minor modification. Permeabilized cells were studied in either intracellular like buffer I (ICL buffer I) (130 mM KCl, 10 mM Tris-MOPS (pH 7.4), 10 μM EGTA-Tris, 1 mM KPi, 5 mM malate, 5 mM sodium glutamate, 1 μM Ca 2+ green 5N) or intracellular like buffer II (ICL buffer II) (130 mM KCl, 10 mM Tris-MOPS (pH 7.4), 10 μM EGTA-Tris, 1 mM KPi, 5 mM malate, 5 mM glutamic acid, 1 μM Ca 2+ green 5N).Importantly, these buffers are identical, with the exception that ICL I contains sodium glutamate and ICL II glutamic acid.…”