Novel parvovirus sublineage in the family of Parvoviridae

Wang, Fang; Wei, Yongwei; Zhu, Chun-Ling; Huang, Xianbo; Xu, Yaping; Liang, Yu; Yu, Xiaohong

doi:10.1007/s11262-010-0506-3

Cited by 54 publications

(49 citation statements)

References 8 publications

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“…Furthermore, other identified parvoviruses from swine, including the recently discovered PPV4 and Cnvirus, formed two distinct branches within other subfamilies of the Parvovirinae (Fig. 1b), consistent with previous studies (Cheung et al, 2010;Wang et al, 2010). Because the closest genetic relative of PBoV-like belonged to the genus Bocavirus, we identified it as a porcine bocavirus (PBoV) strain.…”

supporting

confidence: 88%

“…A total of 29 nearly full-length genome sequences representing different parvovirus species of the subfamily Parvovirinae, including Parvovirus (HPV, MPV, LuIII, MVM, CPV, FPV and PPV1), Amdovirus (AMDV), Bocavirus (CnMV, BPV, HBoV1-4 and GBoV1), Erythrovirus (B19, SPV2, BPV3 and ChPV), Dependovirus (MDPV and GPV) and Hokovirus (PARV4, BHoV and PHoV) were used in this analysis. In addition, two novel porcine parvoviruses, PPV4 and Cnvirus (PPV-H1), recently discovered in piglets but not classified with any known parvovirus species of the subfamily Parvovirinae (Cheung et al, 2010;Wang et al, 2010), were also included. As shown in Fig.…”

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confidence: 99%

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Complete coding sequences and phylogenetic analysis of porcine bocavirus

Zeng

Wang

Fang

et al. 2011

Journal of General Virology

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Here we report, for the first time, the nearly full-length genome sequence of porcine bocavirus (PBoV), a recently discovered parvovirus from pigs. Phylogenetic trees based on this genome sequence showed that PBoV belongs to the branch containing the genus Bocavirus, which comprises canine minute virus (CnMV), bovine parvovirus, gorilla bocavirus and human bocavirus (HBoV), and was most closely related to the group containing CnMV. PBoV was predicted to contain three potential ORFs encoding the non-structural protein NS1, the characteristic NP1 protein and the capsid protein VP1/VP2, with an organization similar to that of known bocaviruses. Interestingly, the NS1 gene of PBoV was more similar in length to the homogeneous gene found in HBoV than to those of other known bocaviruses. In addition, highly conserved unique splicedonor and -acceptor sites were identified in the NS1 gene of HBoV and PBoV.The family Parvoviridae is classified into two subfamilies: the subfamily Parvovirinae, which infects vertebrates, and the subfamily Densovirinae, which infects arthropods. Based on a report published by the International Committee on Taxonomy of Viruses (ICTV), the subfamily Parvovirinae is divided into five genera: Parvovirus, Erythrovirus, Dependovirus, Amdovirus and Bocavirus (Tattersall et al., 2005). Recently, Hokovirus was proposed as a new genus of the subfamily Parvovirinae (Adlhoch et al., 2010;Lau et al., 2008).Bocavirus, one genus of the subfamily Parvovirinae, is a non-enveloped, autonomously replicating, single-stranded DNA virus containing a genome of approximately 5 kb in length, and has close relationships with respiratory and enteric infections in humans and animals (Allander et al., 2005;Chen et al., 1986Chen et al., , 1988 Chen et al., , 2010 Kapoor et al., 2010a, b;Manteufel & Truyen, 2008;Schwartz et al., 2002). The genus Bocavirus comprises four members: bovine parvovirus (BPV), canine minute virus (CnMV), human bocavirus (HBoV) and gorilla bocavirus (GBoV), and has gained special attention because of HBoV, which causes respiratory diseases in children. In the field of veterinary medicine, BPV and CnMV were discovered in the 1960s and they share about 40 % sequence identity (Schwartz et al., 2002).Using random amplification and large-scale sequencing technology, a novel porcine parvovirus was recently discovered in Swedish pigs with post-weaning multisystemic wasting syndrome (PMWS) (Blomström et al., 2009). Based on partial nucleotide sequencing, this novel porcine parvovirus was proposed to be a porcine boca-like virus (PBoV-like). Subsequent studies revealed the high prevalence of this novel PBoV-like in weaning piglets with respiratory tract symptoms or PMWS, and even in pigs without PMWS (Blomström et al., 2010;Zhai et al., 2010). However, to date, the complete genomic sequence for PBoV-like has not been reported.In this study, we first detected the prevalence of this novel PBoV-like from clinical samples collected in central China. Using an established PCR, which amplifies a 496 bp fragme...

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confidence: 88%

mentioning

confidence: 99%

Complete coding sequences and phylogenetic analysis of porcine bocavirus

Zeng

Wang

Fang

et al. 2011

Journal of General Virology

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“…Contig SC5510 had an amino acid identity of 51% with VP1 of H-1 parvovirus, which belongs to the genus Parvovirus, and SC2718 had an amino acid identity of 67% with NS1 of human parvovirus B19, a member of the genus Erythrovirus. Contigs SC462 and SC2596 both had an amino acid identity of 51% to NS1 of parvovirus YX-2010/CHN, which was recently isolated in swine serum (44). Contig SC630 had 41% amino acid identity with VP1 of porcine bocavirus 2 pig/ZJD/China/2006, genus Bocavirus, which was recently identified from swine stool samples (9).…”

Section: Solexa Sequencing Readsmentioning

confidence: 99%

Metagenomic Analysis of Viruses from Bat Fecal Samples Reveals Many Novel Viruses in Insectivorous Bats in China

Yang

et al. 2012

J Virol

200

183

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bIncreasing data indicate that bats harbor diverse viruses, some of which cause severe human diseases. In this study, sequenceindependent amplification and high-throughput sequencing (Solexa) were applied to the metagenomic analysis of viruses in bat fecal samples collected from 6 locations in China. A total of 8,746,417 reads with a length of 306,124,595 bp were obtained. Among these reads, 13,541 (0.15%) had similarity to phage sequences and 9,170 (0.1%) had similarity to eukaryotic virus sequences. A total of 129 assembled contigs (>100 nucleotides) were constructed and compared with GenBank: 32 contigs were related to phages, and 97 were related to eukaryotic viruses. The most frequent reads and contigs related to eukaryotic viruses were homologous to densoviruses, dicistroviruses, coronaviruses, parvoviruses, and tobamoviruses, a range that includes viruses from invertebrates, vertebrates, and plants. Most of the contigs had low identities to known viral genomic or protein sequences, suggesting that a large number of novel and genetically diverse insect viruses as well as putative mammalian viruses are transmitted by bats in China. This study provides the first preliminary understanding of the virome of some bat populations in China, which may guide the discovery and isolation of novel viruses in the future. Bats, the only flying mammal and the second most diverse group of mammals, are natural reservoirs of many emerging viruses (7, 38). Some bat viruses cause severe human diseases, such as lyssavirus, Hendra virus, Nipah virus, Ebola virus, and Marburg virus (16,23,29,41,47). Most of the known bat viruses have been discovered in apparently healthy bats (10,14,19,20,26,34,49). When bats are experimentally infected with henipavirus or the rabies virus, the bats shed the virus but do not produce any clinical syndrome like those observed in other animals and humans (1,17,30,40). This phenomenon may be due to the adaption of viruses to their host species, preinfection with related nonpathogenic viruses, or some unique characteristics of the bat immune system to viral infection. Considering the species richness and wide geographic distribution of bats, bats may be a source of many additional unknown viruses.The techniques for isolating viruses are straightforward but are not always successful because the majority of viruses are unable to grow in cell culture due to a lack of susceptible cell lines, a low titer of virus, or toxicity of environmental samples. PCR assays are sequence-dependent and extremely sensitive and rapid but have been complicated by the lack of a universally conserved gene or gene marker for all types of viruses. Recently, the sequence-independent amplification of viral nucleic acids followed by shotgun sequencing or pyrosequencing has been used to discover an enormous diversity of virus species and genotypes in marine and fresh water (2,6,12,37,39,45), human feces (3, 5, 43), animal tissues, and bat feces (11,13,24,32,42).In this study, six bat fecal samples were collected from six locations...

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“…All samples were found to be negative for the pathogens mentioned above and a nonspecific etiologic agent associated with sow abortion was detected. To explore whether there were any unknown viruses present in the aborted pig fetuses, a SISPA experiment was conducted [21,22]. The sequencing reads were obtained and subjected to BLAST homology searching with sequences deposited in GenBank (BLASTx and tBLASTx programs; http://blast.ncbi.nlm.nih.gov.blast.…”

Section: Resultsmentioning

confidence: 99%

“…Two microliters of the ligation reaction were used to prime PCRs containing 50 pmol of Taq24 and 2.5 units of Taq polymerase (Promega Madison, Wisconsin, USA). Cycling conditions were as follows: 40 cycles, which consisting of 94°C for 1 min and 72°C for 3 min [21,22]. The Amplified PCR products were then analyzed by agarose gel electrophoresis.…”

Section: Sispamentioning

confidence: 99%

Identification and genomic characterization of a novel porcine parvovirus (PPV6) in china

Qiao

Han

et al. 2014

Virol J

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Background: Parvoviruses are classified into two subfamilies based on their host range: the Parvovirinae, which infect vertebrates, and the Densovirinae, which mainly infect insects and other arthropods. In recent years, a number of novel parvoviruses belonging to the subfamily Parvovirinae have been identified from various animal species and humans, including human parvovirus 4 (PARV4), porcine hokovirus, ovine partetravirus, porcine parvovirus 4 (PPV4), and porcine parvovirus 5 (PPV5).

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Novel parvovirus sublineage in the family of Parvoviridae

Cited by 54 publications

References 8 publications

Complete coding sequences and phylogenetic analysis of porcine bocavirus

Complete coding sequences and phylogenetic analysis of porcine bocavirus

Metagenomic Analysis of Viruses from Bat Fecal Samples Reveals Many Novel Viruses in Insectivorous Bats in China

Identification and genomic characterization of a novel porcine parvovirus (PPV6) in china

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