2017
DOI: 10.1159/000481799
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Novel PCR Assays for the Detection of Biological Agents Responsible for Wheat Rust Diseases:<b><i> Puccinia triticina</i></b> and <b><i>Puccinia striiformis</i></b> f. sp. <b><i>tritici</i></b>

Abstract: The species Puccinia triticina (Pt) and Puccinia striiformis f. sp. tritici (Pst) are devastating cereal pathogens that cause leaf and stripe rust diseases. We developed PCR assays for the species-specific detection of Pt and Pst, 2 biological agents that cause wheat rust disease. For each pathogen, we validated 3 primer sets that target the second largest subunits of the RNA polymerase II (rpb2) and β-tubulin 1 (tub1) genes. The specificities of the primers were verified using naturally infected plant materia… Show more

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Cited by 5 publications
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