Novel Plasmid-Encoded Class C β-Lactamase (MOX-2) in
            <i>Klebsiella pneumoniae</i>
            from Greece

Raskine, Laurent; Borrel, Isabelle; Barnaud, Guilène; Boyer, Sophie; Hanau-Berçot, Béatrice; Gravisse, J.; Labia, Roger; Arlet, Guillaume; Sanson-Le-Pors, Marie-José

doi:10.1128/aac.46.7.2262-2265.2002

Cited by 16 publications

(12 citation statements)

References 18 publications

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“…FOX-3 and MOX-2 ␤-lactamases have been detected in K. pneumoniae isolates from Italy (26) and Greece (27), respectively. However, these enzymes were not previously reported in Tunisia; hence, this is the first report of FOX-3 and MOX-2 ␤-lactamase in this country.…”

Section: Resultsmentioning

confidence: 99%

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Cooccurrence of Multiple AmpC β-Lactamases in Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis in Tunisia

Chérif

Saïdani

Decré

et al. 2016

Antimicrob Agents Chemother

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e Over a period of 40 months, plasmid-mediated AmpC ␤-lactamases were detected in Tunis, Tunisia, in 78 isolates (0.59%) of Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis. In 67 isolates, only one ampC gene was detected, i.e., bla CMY-2-type (n ‫؍‬ 33), bla ACC (n ‫؍‬ 23), bla DHA (n ‫؍‬ 6) or bla EBC (n ‫؍‬ 5). Multiple ampC genes were detected in 11 isolates, with the following distribution: bla MOX-2 , bla FOX-3 , and bla CMY-4/16 (n ‫؍‬ 6), bla FOX-3 and bla MOX-2 (n ‫؍‬ 3), and bla CMY-4 and bla MOX-2 (n ‫؍‬ 2). A great variety of plasmids carrying these genes was found, independently of the species and the bla gene. If the genetic context of bla CMY-2-type is variable, that of bla MOX-2 , reported in part previously, is unique and that of bla FOX-3 is unique and new.A mpC ␤-lactamases are cephalosporinases that are poorly inhibited by clavulanic acid (1). AmpC production is one of the mechanisms of resistance to ␤-lactams in enterobacteria, conferring resistance to all ␤-lactams except fourth-generation cephalosporins and carbapenems (2). The genes encoding these enzymes are chromosome or plasmid borne (3).Most plasmid-borne AmpC (pAmpC) ␤-lactamase genes are derived from chromosomal genes that have been mobilized onto plasmids. Based on sequence similarities with species-specific AmpC enzymes, pAmpC variants are classified into five evolutionary groups: the CIT variants (CMY-2 types) originating in Citrobacter freundii, the Enterobacter sp. EBC variants (ACT-1 type, MIR-1), the Morganella morganii DHA variants, the Hafnia alvei ACC variants, and the Aeromonas sp. FOX and MOX variants (1, 2). Several genetic elements are involved in the mobilization of pAmpC ␤-lactamase genes, e.g., the insertion sequences IS26 (CMY-13), ISEcp1 (CMY-2-type, ACC-1-type), and ISCR1, associated with complex integrons (DHA-1 type) (1, 2).The geographic scattering of the different types of pAmpC shows that the CMY-2 type is the most frequent, particularly in Europe (e.g., in France, Spain, Italy,), in Canada, Argentina, and Tunisia (8-10), and in Korea and China (11,12). These pAmpC ␤-lactamases were detected among Enterobacteriaceae, especially in Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, Salmonella enterica, and Proteus mirabilis, and also in naturally AmpC-producing species such as Enterobacter cloacae, Enterobacter aerogenes, and C. freundii (13-15).The pAmpC genes have also been reported in animals. Indeed, in the United States, CMY-2-producing E. coli strains have been found among canine clinical isolates (16). Clinical disease associated with AmpC-producing E. coli in dogs in Australia was first reported in 2006 (17). More recently, a survey of clinical isolates of E. coli from dogs and horses in the Netherlands revealed that 2% of them were AmpC producers (8). Furthermore, a Swedish study has described CMY-2-producing E. coli isolated from broilers (18). Such enzymes have been found in Klebsiella isolates from companion animals in Italy (19). In Tunisia, CMY-2-producing E. coli isolate...

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Section: Resultsmentioning

confidence: 99%

“…Analysis of the genetic environment of bla MOX-2 by cloning and (27). The DNA insert carrying bla MOX-2 was sequenced and four additional open reading frames (ORFs) were identified (Fig.…”

Section: Resultsmentioning

confidence: 99%

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Cooccurrence of Multiple AmpC β-Lactamases in Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis in Tunisia

Chérif

Saïdani

Decré

et al. 2016

Antimicrob Agents Chemother

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“…We therefore determined the ceftazidime and cefepime MICs for strains Ec1, Kp1, and Cf1 and also for 15 isolates of the Enterobacteriaceae expressing a broad range of AmpC-type ␤-lactamases by using the Etest diffusion method (AB Biodisk) on agar plates containing cefoxitin concentrations of 0.25, 1, and 4 g/ml (2,6,13,20,21,23,30,36). The results confirmed the powerful inhibitory action of cefoxitin and its specificity for ACC-1, as cefoxitin did not reduce the ceftazidime or cefepime MICs for the other strains tested.…”

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First Detection of the Ambler Class C 1 AmpC β-Lactamase in Citrobacter freundii by a New, Simple Double-Disk Synergy Test

et al. 2006

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We report on the first detection of an AmpC-type Ambler class C 1 (ACC-1) ␤-lactamase in Citrobacter freundi isolated from a patient also harboring ACC-1-producing Escherichia coli and Klebsiella pneumoniae. We propose a simple cefoxitin-based double-disk synergy test (DDST) for the specific detection of ACC-1 in members of the family Enterobacteriaceae, including natural AmpC producers, in association with a cloxacillinbased DDST as a first-line AmpC-type ␤-lactamase screening test.

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“…The remaining plasmid-associated ampC group with sequences similar to that of bla MOX-1 exhibit ϳ70% identity to the genes of Aeromonas spp. (26).…”

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Promoter Sequences Necessary for High-Level Expression of the Plasmid-AssociatedampCβ-Lactamase Genebla_MIR-1

Reisbig

Hanson

2004

Antimicrob Agents Chemother

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Little is known about mechanisms involved in high-level expression of plasmid-associated ampC genes. The sequence for bla MIR-1 has been elucidated, and the gene is not inducible. Although the sequence for the promoter (prA) that drives expression of Enterobacter cloacae chromosomal ampC is present upstream of bla MIR-1 , high-level expression from bla MIR-1 is directed from a hybrid promoter (prB) located further upstream of prA. The purpose of this study was to determine the influence of each promoter on bla MIR-1 expression and ␤-lactam resistance. RNA expression by deletion clones with both promoters was measured and compared to that by clones in which ؊35 and/or ؊10 elements of prA and/or prB were altered. Primer extension revealed two start sites for bla MIR-1 transcription. Expression of bla MIR-1 in clones with both promoters was 171-fold higher than that in clones carrying only prA. In addition, bla MIR-1 expression from prA increased 11-fold in the presence of the prB ؊10 element compared to expression driven from prA alone. Ceftazidime and cefotaxime MICs increased 42-and 64-fold, respectively, for the clone expressing bla MIR-1 from both promoters compared to expression from prA alone. The upstream promoter prB of bla MIR-1 is solely responsible for high-level expression required for cefotaxime and ceftazidime resistance. These data suggest that resistance to extended-spectrum cephalosporins mediated by noninducible plasmid-associated ampC genes requires the formation of novel promoter elements that are capable of increasing ampC expression.

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Novel Plasmid-Encoded Class C β-Lactamase (MOX-2) in Klebsiella pneumoniae from Greece

Cited by 16 publications

References 18 publications

Cooccurrence of Multiple AmpC β-Lactamases in Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis in Tunisia

Cooccurrence of Multiple AmpC β-Lactamases in Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis in Tunisia

First Detection of the Ambler Class C 1 AmpC β-Lactamase in Citrobacter freundii by a New, Simple Double-Disk Synergy Test

Promoter Sequences Necessary for High-Level Expression of the Plasmid-AssociatedampCβ-Lactamase Genebla_MIR-1

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Novel Plasmid-Encoded Class C β-Lactamase (MOX-2) in Klebsiella pneumoniae from Greece

Cited by 16 publications

References 18 publications

Cooccurrence of Multiple AmpC β-Lactamases in Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis in Tunisia

Cooccurrence of Multiple AmpC β-Lactamases in Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis in Tunisia

First Detection of the Ambler Class C 1 AmpC β-Lactamase in Citrobacter freundii by a New, Simple Double-Disk Synergy Test

Promoter Sequences Necessary for High-Level Expression of the Plasmid-AssociatedampCβ-Lactamase GeneblaMIR-1

Contact Info

Product

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Promoter Sequences Necessary for High-Level Expression of the Plasmid-AssociatedampCβ-Lactamase Genebla_MIR-1