Novel plasmid-mediated colistin resistance gene mcr-7.1 in Klebsiella pneumoniae

Yang, Yongqiang; Li, Yunxia; Lei, Changwei; Zhang, Anyun

doi:10.1093/jac/dky111

Cited by 319 publications

(209 citation statements)

References 25 publications

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“…it has to be considered that although we did not find mcr-2 in our study, up to 8 genetic 296 determinants for colistin resistance have been described [60][61][62]. Therefore, a search for 297 more genetic determinants should be conducted to better understand the epidemiology 298 of colistin resistance in poultry from Ecuador.…”

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confidence: 69%

Characterization of cefotaxime resistant Escherichia coli isolated from broiler farms in Ecuador

Vinueza-Burgos

Ortega-Paredes

Narváez

et al. 2018

Preprint

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Antimicrobial resistance (AR) is a worldwide concern. Up to a 160% increase in antibiotic usage in food animals is expected in Latin American countries. The poultry industry is an increasingly important segment of food production and contributor to AR. The objective of this study was to evaluate the prevalence, AR patterns and the characterization of relevant resistance genes in Extended Spectrum β-lactamases (ESBL) and AmpC E. coli from large poultry farms in Ecuador. Sampling was performed from June 2013 to July 2014 in 6 slaughterhouses that slaughter broilers from 115 farms totaling 384 flocks. Each sample of collected caeca was streaked onto TBX agar supplemented with cefotaxime (3 mg/l). In total, 176 isolates were analyzed for antimicrobial resistance patterns by the disk diffusion method and for blaCTX-M, blaTEM, blaCMY, blaSHV, blaKPC, and mcr-1 by PCR and sequencing. ESBL and AmpC E. coli were found in 362 flocks (94.3%) from 112 farms (97.4%). We found that 98.3% of the isolates were multi-resistant to antibiotics. Low resistance was observed for ertapenem and nitrofurantoin. The most prevalent ESBL genes were the blaCTX-M (90.9%) blaCTX-M-65, blaCTX-M-55 and blaCTX-M-3 alleles. Most of the AmpC strains presented the blaCMY-2 gene. Three isolates showed the mcr-1 gene. Poultry production systems represent a hotspot for antimicrobial resistance in Ecuador, possibly mediated by the extensive use of antibiotics. Monitoring this sector in national and regional plans of antimicrobial resistance surveillance should therefore be considered.

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mentioning

confidence: 69%

Characterization of cefotaxime resistant Escherichia coli isolated from broiler farms in Ecuador

Vinueza-Burgos

Ortega-Paredes

Narváez

et al. 2018

Preprint

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“…coli that carried the colistin resistance gene (mcr-1) (Liu et al, 2016) were identified. Until now, 9 subtypes of mcr (Liu et al, 2016;Xavier et al, 2016;AbuOun et al, 2017;Borowiak et al, 2017;Carattoli et al, 2017;Yin et al, 2017;Wang X. et al, 2018;Yang et al, 2018;Carroll et al, 2019) and 21 subtypes of bla NDM have been published with papers. The discovery of these two types of resistance genes that can be horizontally transferred via plasmids has made researchers aware of the post-antibiotic era.…”

Section: Introductionmentioning

confidence: 99%

Retrospective Screening and Analysis of mcr-1 and blaNDM in Gram-Negative Bacteria in China, 2010–2019

Fan

Duan

et al. 2020

Front. Microbiol.

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Currently, Gram-negative bacteria have developed multidrug and broad-spectrum drug resistance, and the numbers of species and strains carrying mcr or bla NDM genes are increasing. In this study, mcr-1 and bla NDM distribution of 12,858 Gramnegative bacteria isolated from wildlife, patients, livestock, poultry and environment in 14 provinces of China from 2010 to 2019 and the antibiotics resistance in regard to polymyxins (polymyxin B and colistin) and carbapenems of positive strains were investigated. A total of 70 strains of 10 species carried the mcr-1 gene, positive rates of patients, livestock and poultry, and environmental strains were 0.62% (36/5,828), 4.07% (29/712), 5.43% (5/92), respectively. Six strains of 3 species carrying the bla NDM gene all came from patients 0.10% (6/5,828). Two new mcr-1 gene variants (GenBank: MK965883, MK965884) were identified, one of which contains premature stop codon. The drug susceptibility results showed that all mcr-1 carriers were sensitive to carbapenems, among which, 66 strains were resistant and 4 were sensitive to polymyxins. The strains with the bla NDM gene had different degrees of resistance to carbapenems and were sensitive to polymyxins. The findings that species carrying mcr-1 or bla NDM genes were limited and mostly normal flora of opportunistic or low pathogenic organisms indicated that transfer of mcr-1 and bla NDM genes between bacteria was relatively limited in China. The none detection among wildlife compared with other sources supports the speculation that the emergence of and increase in polymyxins and carbapenem-resistant strains was mainly related to the selective pressure of antibiotics.

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“…In 2017, the following four genes were described: (i) mcr-3 in E. coli from pigs in China (Yin et al, 2017); (ii) mcr-4 in Salmonella and E. coli isolated from pigs in Italy, Spain, and Belgium (Carattoli et al, 2017); (iii) mcr-5 in Salmonella Paratyphi B from poultry in Germany (Borowiak et al, 2017); and (iv) mcr-6 (originally termed mcr-2.2 variant) in Moraxella pluranimalium from pigs at slaughter in the United Kingdom (AbuOun et al, 2018). The next year, mcr-7 was detected in Klebsiella pneumoniae from chickens and mcr-8 in K. pneumoniae from pigs, chickens, and humans in China Yang et al, 2018). In 2019, the mcr-9 gene was described in CT-susceptible Salmonella Typhimurium isolated from a patient in the United States in 2010.…”

Section: Introductionmentioning

confidence: 99%

Dissemination and Comparison of Genetic Determinants of mcr-Mediated Colistin Resistance in Enterobacteriaceae via Retailed Raw Meat Products

et al. 2019

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The global food chain may significantly promote the dissemination of bacteria resistant to antibiotics around the world. This study was aimed at determining the prevalence and genetic characteristics of Enterobacteriaceae with mcr-mediated colistin (CT) resistance in retail meat of different origins. Bacteria of the Enterobacteriaceae family carrying the mcr-1 gene were detected in 21% (18/86) of the examined samples, especially in turkey meat and liver originating from EU and non-EU countries (19%) and in rabbit meat imported from China (2%). The examined samples of the meat and liver of chicken and other poultry and of pork and beef were negative for the presence of bacteria carrying the mcr-1 to mcr-5 genes. A huge number of isolates belonging to Escherchia coli (n = 54), Klebsiella pneumoniae (n = 6), and Citrobacter braakii (n = 1) carrying the mcr-1 gene were obtained. Despite the high heterogeneity of the tested isolates, the mcr-1 gene was localized on only three types of plasmids (IncX4, IncHI2, and IncI2). The most frequent type of plasmid was IncX4, which carried the mcr-1 gene in 77% of E. coli and K. pneumoniae isolates from turkey meat and liver samples from the Czechia, Germany, Poland, and Brazil. Our findings indicate highly probable interspecies transfer of IncX4 and IncI2 plasmids within one meat sample. The co-resistance of plasmid-mediated CT resistance encoded by the mcr-1 and ESBL genes was detected in 18% of the isolates. Another noteworthy finding was the fosA3 gene coding for fosfomycin resistance in a multidrug-resistant isolate of E. coli from rabbit meat imported from China. The observed high level of Enterobacteriaceae with plasmids carrying the mcr-1 gene in retail meat reflects the need for Europe-wide monitoring of mcr-mediated CT resistance throughout the whole food chain.

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Novel plasmid-mediated colistin resistance gene mcr-7.1 in Klebsiella pneumoniae

Abstract: We identified a novel plasmid-mediated colistin resistance gene named mcr-7.1 in K. pneumoniae in China. The prevalence of mcr-7.1 in various species of human and animal origin needs to be investigated immediately.

Cited by 319 publications

References 25 publications

Characterization of cefotaxime resistant Escherichia coli isolated from broiler farms in Ecuador

Characterization of cefotaxime resistant Escherichia coli isolated from broiler farms in Ecuador

Retrospective Screening and Analysis of mcr-1 and blaNDM in Gram-Negative Bacteria in China, 2010–2019

Dissemination and Comparison of Genetic Determinants of mcr-Mediated Colistin Resistance in Enterobacteriaceae via Retailed Raw Meat Products

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