Novel Poly(butylene adipate-co-terephthalate)-degrading Bacillus sp. JY35 from wastewater sludge and its broad degradation of various bioplastics

Cho, Jang Yeon; Park, Sol Lee; Kim, Su Hyun; Jung, Hee Ju; Cho, Do Hyun; Kim, Byung Chan; Bhatia, Shashi Kant; Gurav, Ranjit; Park, See‐Hyoung; Park, Kyungmoon; Yang, Yung‐Hun

doi:10.1016/j.wasman.2022.03.003

Cited by 35 publications

(12 citation statements)

References 42 publications

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“…In the culture medium, first, some microbial aggregates composed of cells and extracellular polymers can adhere to each other or adhere to the surface of MPs to form complex biofilms [ 50 , 51 , 52 ] ( Figure 6 a). The biofilm includes one or more bacteria, which is a biological community with diversity in phylogeny and function [ 53 ].…”

Section: Resultsmentioning

confidence: 99%

Biodeterioration of Microplastics by Bacteria Isolated from Mangrove Sediment

Ren

2023

Toxics

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As a kind of ubiquitous emerging pollutant, microplastics (MPs) are persistent in the environment and have a large impact on the ecosystem. Fortunately, some microorganisms in the natural environment can degrade these persistent MPs without creating secondary pollution. In this study, 11 different MPs were selected as carbon sources to screen the microorganisms for degradable MPs and explore the possible mechanism of degradation. After repeated domestication, a relatively stable microbial community was obtained after approximately 30 days later. At this time, the biomass of the medium ranged from 88 to 699 mg/L. The growth of bacteria with different MPs ranged from 0.030 to 0.090 optical density (OD) 600 of the first generation to 0.009–0.081 OD 600 of the third generation. The weight loss method was used to determine the biodegradation ratios of different MPs. The mass losses of polyhydroxybutyrate (PHB), polyethylene (PE), and polyhydroxyalkanoate (PHA) were relatively large, at 13.4%, 13.0%, and 12.7%, respectively; these figures for polyvinyl chloride (PVC) and polystyrene (PS) were relatively slight, 8.90% and 9.10%, respectively. The degradation half-life (t1/2) of 11 kinds of MPs ranges from 67 to 116 days. Among the mixed strains, Pseudomonas sp., Pandoraea sp., and Dyella sp. grew well. The possible degradation mechanism is that such microbial aggregates can adhere to the surface of MPs and form complex biofilms, secrete extracellular and intracellular enzymes, etc., break the hydrolyzable chemical bonds or ends of molecular chains by attacking the plastic molecular chains, and produce monomers, dimers, and other oligomers, leading to the reduction of the molecular weight of the plastic itself.

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Section: Resultsmentioning

confidence: 99%

Biodeterioration of Microplastics by Bacteria Isolated from Mangrove Sediment

Ren

2023

Toxics

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“…After 10 days, films that were recovered from culture medium had M n of 2.88 × 10 4 and M w of 6.82 × 10 4 . However, PDI values did not show significant changes like in the case of PHA and PBAT degradation [ 32 , 40 ]. Usually, PBS is degraded because enzymes produced from microorganisms attack the ester bond in the main chain of PBS.…”

Section: Resultsmentioning

confidence: 99%

“…The flow rate of the mobile phase was maintained at 0.6 mL/min using 0.004 mol/L sulfuric acid (H 2 SO 4 ). The oven temperature was set to 60 °C during the operation [ 40 ].…”

Section: Methodsmentioning

confidence: 99%

Acceleration of Polybutylene Succinate Biodegradation by Terribacillus sp. JY49 Isolated from a Marine Environment

Kim

Cho

et al. 2022

Polymers

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Polybutylene succinate (PBS) is a bioplastic substitute for synthetic plastics that are made from petroleum-based products such as polyethylene and polypropylene. However, the biodegradation rate of PBS is still low and similar to that of polylactic acid (PLA). Moreover, our knowledge about degrader species is limited to a few fungi and mixed consortia. Here, to identify a bacterial degrader to accelerate PBS degradation, we screened and isolated Terribacillus sp. JY49, which showed significant degradability. In order to optimize solid and liquid culture conditions, the effect of factors such as temperature, additional carbon sources, and salt concentrations on degradation was confirmed. We observed a degradation yield of 22.3% after 7 days when adding 1% of glucose. Additionally, NaCl was added to liquid media, and degradation yield was decreased but PBS films were broken into pieces. Comparing the degree of PBS degradation during 10 days, the degradation yield was 31.4% after 10 days at 30 °C. Alteration of physical properties of films was analyzed by using scanning electron microscopy (SEM), gel permeation chromatography (GPC), and Fourier transform infrared (FT-IR). In addition, Terribacillus sp. JY49 showed clear zones on poly(butylene adipate-co-terephthalate) (PBAT), polycaprolactone (PCL), and copolymers such as P(3HB-co-3HV) and P(3HV-co-4HB), exhibiting a broad spectrum of degradation activities on bioplastics. However, there was no significant difference in absorbance when esterase activity was examined for different types of bioplastics. Overall, Terribacillus sp. JY49 is a potential bacterial strain that can degrade PBS and other bioplastics, and this is the first report of Terribacillus sp. as a bioplastic degrader.

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“…Then, each film was cultivated with the precultured Microbulbifer sp. SOL66 in 5 mL of MB liquid media [21]. After 2 days of cultivation at 37 • C, 200 rpm, optical density was measured with the same process described in the previous section.…”

Section: Degradation Of Phb Containing Various Plasticizers Under The...mentioning

confidence: 99%

Finding a Benign Plasticizer to Enhance the Microbial Degradation of Polyhydroxybutyrate (PHB) Evaluated by PHB Degrader Microbulbifer sp. SOL66

et al. 2022

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As a biodegradable plastic, polyhydroxybutyrate (PHB) has relatively poor mechanical properties, preventing its wider use. Various plasticizers have been studied to improve the mechanical properties of PHB; however, due to the slow degradation speed in the soil environment and lack of evaluation methods, studies on the degradation of PHB with plasticizers are rarely reported. In this study, by applying Microbulbifer sp. SOL66, which is able to degrade PHB very quickly, a benign plasticizer was evaluated with good properties and good degradability, not inhibiting microbial activities. Eight different plasticizers were applied with PHB and Microbulbifer sp. SOL66, PHB film containing 10% and 20% tributyl citrate showed significant biodegradability of PHB. It was confirmed that tributyl citrate could increase the speed of PHB degradation by Microbulbifer sp. SOL66 by 88% at 1 day, although the degree of degradation was similar after 3 days with and without tributyl citrate. By the analysis of microbial degradation, physical, chemical, and mechanical properties, tributyl citrate was shown not only to improve physical, chemical, and mechanical properties but also the speed of microbial degradation.

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Novel Poly(butylene adipate-co-terephthalate)-degrading Bacillus sp. JY35 from wastewater sludge and its broad degradation of various bioplastics

Cited by 35 publications

References 42 publications

Biodeterioration of Microplastics by Bacteria Isolated from Mangrove Sediment

Biodeterioration of Microplastics by Bacteria Isolated from Mangrove Sediment

Acceleration of Polybutylene Succinate Biodegradation by Terribacillus sp. JY49 Isolated from a Marine Environment

Finding a Benign Plasticizer to Enhance the Microbial Degradation of Polyhydroxybutyrate (PHB) Evaluated by PHB Degrader Microbulbifer sp. SOL66

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