Novel poly(ε-caprolactone)/amino-functionalized tannin electrospun membranes as scaffolds for tissue engineering

Martins, Alessandro F.; Facchi, Suelen Pietrobon; Câmara, Paulo C.F. da; Camargo, Samira Esteves Afonso; Camargo, Carlos Henrique; Popat, Ketul C.; Kipper, Matt J.

doi:10.1016/j.jcis.2018.04.060

Cited by 76 publications

(51 citation statements)

References 39 publications

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“…Both poly(ε-caprolactone) and poly(ε-caprolactone)-dexamethasone solutions (5.0 mL) were obtained in a vortex stirrer at room temperature for 1.0 h. Poly(ε-caprolactone) and poly(ε-caprolactone)dexamethasone solutions were separately transferred to a 10 mL glass syringe and connected to an 18-gauge blunt stainless-steel needle charged by a high voltage direct current (DC) power supply (Gamma High Voltage Research) at 17 kV (20 ± 2 o C and 22% relative humidity). 34 The tip to collector distance was set at 12.5 cm, and the solutions are pumped (2.5 mL h -1 ) in a Kent Scientific Genie Plus (Torrington, USA) syringe pump. As-obtained poly(εcaprolactone) and poly(ε-caprolactone)-dexamethasone membranes were characterized.…”

Section: Methodsmentioning

confidence: 99%

“…Before seeding the cells on the samples, the adiposederived mesenchymal stem cells (ADSC) are cultured according to the previous methodology reported by Martins et al 34 (see Supplementary Information (SI) section).…”

Section: Cell Culture Assaysmentioning

confidence: 99%

“…Cell viability is determined by a CellTiter-Blue ® cell viability assay (Promega G808A, Madison, USA) according to the manufacturer's instructions and a previously reported methodology. 34 After 1 and 7 days, 50 µL CellTiter-Blue ® dye is added to 500 µL cell culture media containing the sample surfaces and incubated for 6 h (37 o C) at 5% CO 2 . The cell viability is determined in a UV-Vis microplate reader (Molecular Devices Spectra Max M3, Sunnyvale, USA) at 570 and 600 nm.…”

Section: Cell Viability Studiesmentioning

confidence: 99%

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Chitosan Imparts Better Biological Properties for Poly(ε-caprolactone) Electrospun Membranes than Dexamethasone

Machado

Roberto

Bonafé

et al. 2019

J. Braz. Chem. Soc.

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Poly(ε-caprolactone), an aliphatic polyester with biodegradability and cytocompatibility, has been used to create scaffolds for tissue engineering purposes. However, the hydrophobicity and low water absorptivity of poly(ε-caprolactone) reduce cell anchorage on their membranes. Here, poly(ε-caprolactone)-based scaffolds were prepared by electrospinning of poly(ε-caprolactone)chitosan blend, and poly(ε-caprolactone)-dexamethasone solution. Chitosan and dexamethasone play an essential role to increase the scaffolding performance of poly(ε-caprolactone)-based electrospun membranes. A poly(ε-caprolactone) membrane without chitosan and dexamethasone did not provide satisfactory results to promote cell culture of adipose mesenchymal stem cells (AMSCs). Compared to the poly(ε-caprolactone)-dexamethasone surface, poly(ε-caprolactone)chitosan membrane imparts better cytoskeletal reorganization, and cell spreading, increasing the strength of cell attachment. Also, poly(ε-caprolactone)-chitosan composite provides strong antimicrobial activity against Pseudomonas aeruginosa (ca. 90% inhibition). Therefore, the poly(ε-caprolactone)-chitosan composite is a better alternative to treat skin diseases and promote skin regeneration than conventional approaches based on dexamethasone.

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Section: Methodsmentioning

confidence: 99%

Section: Cell Culture Assaysmentioning

confidence: 99%

Section: Cell Viability Studiesmentioning

confidence: 99%

See 1 more Smart Citation

Chitosan Imparts Better Biological Properties for Poly(ε-caprolactone) Electrospun Membranes than Dexamethasone

Machado

Roberto

Bonafé

et al. 2019

J. Braz. Chem. Soc.

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“…Bu durumda tamir olan yeni deri dokusunun zarar görmesine ve yara iyileşme sürecinin uzamasına neden olmaktadır (24). Bunun yanı sıra diğer alifatik polyesterlerde olduğu gibi yüksek hidrofobik özelliği PCL'nin yara örtü malzemesi olarak tek başına kullanımını sınırlamaktadır [25]. Polihidroksi alkonatlar (PHA), mikroorganizmalar tarafından üretilen biyobozunur doğal termoplastik alifatik polyesterlerdir ve yüksek biyouyumluluk özellikleri sebebiyle biyomalzeme olarak kullanılmaktadırlar [26].…”

Section: Introductionunclassified

“…Örneğin literatürde yer alan bir çalışmada Tohidi ve arkadaşları elektroeğrilmiş amoksilin yüklü halloysit nanokil içeren PLGA/kitosan membranlar hazırlamışlar ve yara örtüsü olarak kullanımını araştırmışlardır [16]. Martins ve arkadaşları, elektroeğrilmiş PCL/aminofonksiyonel tanen membranlar hazırlamışlar ve yara örtüsü olarak performansını değerlendirmişlerdir [25]. Adeli-Sardou ve arkadaşları elektroeğirme yöntemi ile PCL/jelatin membranlar hazırlamışlar, bu membran içerisine kına yaprağı ekstratı olan lawsone yüklemişler ve deri doku mühendisliği için doku iskelesi olarak kullanımını araştırmışlardır [29].…”

Section: Introductionunclassified

The Preparation and Characterization of Electrospun PCL/PHBV Membranes as Wound Dressing Material

İşoğlu

2019

Bitlis Eren Üniversitesi Fen Bilimleri Dergisi

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2-propanol (HFIP) ve bunların karışımları) kullanılarak elektroeğirme tekniği ile fibroz yapıda membranlar hazırlanmıştır. Tüm elektroeğrilmiş membranlar Fourier Dönüşümlü Kızılötesi Spektroskopisi (FT-IR), Diferansiyel Taramalı Kalorimetre (DSC) ve Taramalı Elektron Mikroskobu (SEM) ile yapı, morfoloji ve ısıl özellikleri açısından karakterize edilmiştir. Ayrıca, absorbsiyon testi ile sıvı tutma kapasiteleri analiz edilmiştir. Karakterizasyon basamağından sonra, seçilen membranların üzerine insan fibroblast hücreleri ekilmiş, in vitro hücre canlılık ve toksisite, MTT testi ile 24, 48 ve 72. saat için analiz edilmiştir. Membranların üzerine ekilen hücrelerin çoğalması 36, 72 ve 120. saat olmak üzere 3 farklı süre için incelenmiş, sonuçlar SEM ile gösterilmiştir. Elde edilen sonuçlar PCL/PHBV (75:25) karışım ile HFIP çözeltisinde elektroeğrilen membranın yara örtü malzemesi olarak kullanılabileceğini göstermiştir.

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Expanding the Repertoire of Electrospinning: New and Emerging Biopolymers, Techniques, and Applications

Madruga

Kipper

2021

Adv Healthcare Materials

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Electrospinning has emerged as a versatile and accessible technology for fabricating polymer fibers, particularly for biological applications. Natural polymers or biopolymers (including synthetically derivatized natural polymers) represent a promising alternative to synthetic polymers, as materials for electrospinning. Many biopolymers are obtained from abundant renewable sources, are biodegradable, and possess inherent biological functions. This review surveys recent literature reporting new fibers produced from emerging biopolymers, highlighting recent developments in the use of sulfated polymers (including carrageenans and glycosaminoglycans), tannin derivatives (condensed and hydrolyzed tannins, tannic acid), modified collagen, and extracellular matrix extracts. The proposed advantages of these biopolymer-based fibers, focusing on their biomedical applications, are also discussed to highlight the use of new and emerging biopolymers (or new modifications to well-established ones) to enhance or achieve new properties for electrospun fiber materials.

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Novel poly(ε-caprolactone)/amino-functionalized tannin electrospun membranes as scaffolds for tissue engineering

Cited by 76 publications

References 39 publications

Chitosan Imparts Better Biological Properties for Poly(ε-caprolactone) Electrospun Membranes than Dexamethasone

Chitosan Imparts Better Biological Properties for Poly(ε-caprolactone) Electrospun Membranes than Dexamethasone

The Preparation and Characterization of Electrospun PCL/PHBV Membranes as Wound Dressing Material

Expanding the Repertoire of Electrospinning: New and Emerging Biopolymers, Techniques, and Applications

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