Novel polymorphisms detected in the prolactin receptor gene of Yili horse (&lt;i&gt;Equus caballus&lt;/i&gt;) by PCR-SSCP

Abula, Reyanggu; Zhang, H.-L.; Chen, Yong; Yao, Xiaoguang

doi:10.22358/jafs/66021/2013

Cited by 2 publications

(2 citation statements)

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“…26 Therefore, optimizing the conditions of PCR-SSCP electrophoresis for each specified type of PCR product is necessary to control the porosity of the gels, amplicons sizes, loaded amplicons, the voltage applied, and other variable parameters. 27 Therefore, several optimization steps should be conducted to circumvent the undesired low resolutions that might occur in PCR-SSCP. The optimization of PCR-SSCP is achieved in a series of experiments that should be performed to increase the resolution of PCR-SSCP, such as polyacrylamide gel concentration (8%-14%), temperature (4-20°C), and voltage (5-10 V/cm).…”

Section: Simplicity Of Usementioning

confidence: 99%

Exploring the Potential and Limitations of PCR-RFLP and PCR-SSCP for SNP Detection: A Review

Hashim

Al-Shuhaib

2019

J Apple Biotechnol Rep

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Polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) and PCR-restriction fragment length polymorphism (PCR-RFLP) are two independent methods used in the post-amplification genotyping of DNA variations. Both techniques are used in a wide range of screening applications to characterize single nucleotide polymorphisms (SNPs). The PCR-SSCP enables the identification of a potentially causative unknown SNP that could not be identified by PCR-RFLP. However, because complicated steps are not required to perform PCR-RFLP, it is used in many applications. On the other hand, PCR-RFLP is easier to process in terms of time and handover experience, the detection of a particular unknown SNP by PCR-SSCP has further chances. The simplicity of PCR-RFLP does not mean that it is better than PCR-SSCP. The reason is the limited ability of PCR-RFLP to detect nucleotide variations, which often go undetected because each restriction enzyme (RE) scans only a few recognition sequences, and other sequences are ignored. Furthermore, the efficacy of PCR-SSCP is sometimes hindered by many optimizations and also lack of experience. As PCR-SSCP allows other sequences within an amplicon to be separated and characterized, the choice between PCR-RFLP and PCR-SSCP is largely dependent on the reason for each genotyping experiment. This review provides a useful guide for comparing PCR-RFLP and PCR-SSCP in terms of their concepts, efficiency, ease of use, interpretation, and sensitivity as well as several other parameters. The comparison is extended to the practical applications of both techniques in terms of their utilization in molecular diagnostics and related applications.

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Section: Simplicity Of Usementioning

confidence: 99%

Exploring the Potential and Limitations of PCR-RFLP and PCR-SSCP for SNP Detection: A Review

Hashim

Al-Shuhaib

2019

J Apple Biotechnol Rep

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“…Однонуклеотидный полиморфизм (SNP) является наиболее распространенной формой генетической изменчивости и источником полезных генетических признаков. SNP в кодирующих областях гена PRLR были обнаружены с помощью полимеразной цепной реакцииодноцепочечного конформационного полиморфизма (SSCP) и секвенирования ДНК на китайских лошадях Йили [42]. Немецкие ученые выявили ассоциации гена рецептора пролактина с воспроизводительными качествами жеребцов ганноверской породы [43].…”

Section: введение / Introductionunclassified

Analysis of polymorphic variants of MSTN, CAST, PRLR genes associations with economically useful qualities of vyatka breed horses

Belousova,

Bass,

Zinovieva

et al. 2023

jour

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The relationship of polymorphic variants of MSTN, CAST, PRLR genes with working qualities and body types of Vyatka horses was studied. The purpose of the research is to assess the genetic and breeding potential of the Vyatka breed horses, as well as to study the relationship between the MSTN, CAST, PRLR genotypes and the economically useful qualities of horses. The method of DNA extraction from Vyatka horse hair follicles using ExtraGene DNA Prep. was applied. When scanning the mutations in the loci MSTN (n=43), CAST (n=41) and PRLR (n=41), DNA amplification the method of allele-specific PCR was performed. The frequency of alleles and genotypes was calculated using MS Excel 10. As a result of research, the dependence of Vyatka horses working qualities on the frequency of occurrence of myostatin MSTN alleles (g.66493737 T>C) was noted. The horses with a higher occurrence of the MSTN/C allele have more productive movements than the individuals with the T/T genotype typical for aborigines. Vyatka horses with the T/T genotype are more versatile, and also show better results in sledding, while the horses with the T/C genotype are better under saddle. The horses with the T/T genotype have the highest bony index but the lowest massiveness index. The MSTN T/T (0.581) homozygous genotype for the "wild" allele predominates in Vyatka horses. A relation ship between body types and the calpastatin gene (CAST) has been revealed for the first time in horse breeding. The horses with the G/A genotype turned out to be the most massive and bony, the horses with the A/A genotype were lighter, the highest frequency of occurrence of the CAST G/A genotype (0.463) was noted, the CAST G/G genotype is rare in the breed (0.171). A relationship between the frequency of occurrence of prolactin receptor genes (PRLR) and body types of horses was not found. The frequency of occurrence of PRLR C/C (0.366) and PRLR G/C (0.390) genotypes is approximately identical, the PRLR G/G genotype is less common (0.244). The study of genes associated with economically useful qualities in all breeding stallions will enable to conduct more efficient breeding, using the desired genotypes, which is important for small breeds.

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Novel polymorphisms detected in the prolactin receptor gene of Yili horse (<i>Equus caballus</i>) by PCR-SSCP

Cited by 2 publications

References 10 publications

Exploring the Potential and Limitations of PCR-RFLP and PCR-SSCP for SNP Detection: A Review

Exploring the Potential and Limitations of PCR-RFLP and PCR-SSCP for SNP Detection: A Review

Analysis of polymorphic variants of MSTN, CAST, PRLR genes associations with economically useful qualities of vyatka breed horses

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