Novel polymorphisms in the bovine <i>β‐lactoglobulin</i> gene and their effects on <i>β</i>‐lactoglobulin protein concentration in milk

Ganai, Nazir Ahmad; Bovenhuis, H.; Arendonk, J.A.M. van; Visker, M.H.P.W.

doi:10.1111/j.1365-2052.2008.01806.x

Cited by 57 publications

(64 citation statements)

References 19 publications

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“…The number of polymorphisms identified in our breeds showed high variability of the BLG promoter region as reported by Sardina et al [37] in goat and by Ganai et al [45] in cattle. Valle del Belice and Comisana breeds had all point mutations (36 SNPs and the deletion) in common, whereas the Pinzirita breed presented 34 SNPs and the deletion, and [30].…”

Section: Identification Of Polymorphisms and Genetic Diversity Indexessupporting

confidence: 83%

Study of polymorphisms in the promoter region of ovine β-lactoglobulin gene and phylogenetic analysis among the Valle del Belice breed and other sheep breeds considered as ancestors

et al. 2011

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The aim of this work was to sequence the promoter region of b-lactoglobulin (BLG) gene in four sheep breeds, in order to identify polymorphisms, infer and analyze haplotypes, and phylogenetic relationship among the Valle del Belice breed and the other three breeds considered as ancestors. Sequencing analysis and alignment of the obtained sequences showed the presence of 36 single nucleotide polymorphisms (SNPs) and one deletion. A total of 22 haplotypes found in ''best'' reconstruction were inferred considering the 37 polymorphic sites identified. Haplotypes were used for the reconstruction of a phylogenetic tree using the Neighbor-Joining algorithm. The number of polymorphisms identified showed high variability within breeds. Analysis of genetic diversity indexes showed that the Sarda breed presented the lowest nucleotide diversity, whereas the Comisana breed presented the highest one. Comparing the nucleotide diversity among breeds, the highest value was obtained between Valle del Belice and Pinzirita breeds, whereas the lowest one was between Valle del Belice and Sarda breeds. Considering that polymorphisms in the promoter region of BLG gene could have a functional role associated with milk composition, the lowest value of nucleotide diversity between Valle del Belice and Sarda breeds may be related to a higher similarity of milk composition of these two breeds compared to the others. Further analyses will be conducted in order to evaluate the possible correlation between the genetic diversity indexes and the BLG content in milk of our breeds.

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Section: Identification Of Polymorphisms and Genetic Diversity Indexessupporting

confidence: 83%

Study of polymorphisms in the promoter region of ovine β-lactoglobulin gene and phylogenetic analysis among the Valle del Belice breed and other sheep breeds considered as ancestors

et al. 2011

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“…In a study by Ganai et al (2009), SNP within the PAEP gene were identified in relation to β-LG concentration. In that study, the A variant of PAEP was shown to have a significant negative effect on relative concentration of β-LG, whereas other studies have shown a negative effect of the B variant on β-LG concentration (Allmere et al, 1998b;Hallén et al, 2008).…”

Section: Major Qtl On Chromosomementioning

confidence: 99%

Bovine chromosomal regions affecting rheological traits in acid-induced skim milk gels

Glantz

Gustavsson

Bertelsen

et al. 2015

Journal of Dairy Science

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The production of fermented milk products has increased worldwide during the last decade and is expected to continue to increase during the coming decade. The quality of these products may be optimized through breeding practices; however, the relations between cow genetics and technological properties of acid milk gels are not fully known. Therefore, the aim of this study was to identify chromosomal regions affecting acid-induced coagulation properties and possible candidate genes. Skim milk samples from 377 Swedish Red cows were rheologically analyzed for acid-induced coagulation properties using low-amplitude oscillation measurements. The resulting traits, including gel strength, coagulation time, and yield stress, were used to conduct a genome-wide association study. Single nucleotide polymorphisms (SNP) were identified using the BovineHD SNPChip (Illumina Inc., San Diego, CA), resulting in almost 621,000 segregating markers. The genome was scanned for putative quantitative trait loci (QTL) regions, haplotypes based on highly associated SNP were inferred, and the additive genetic effects of haplotypes within each QTL region were analyzed using mixed models. A total of 8 genomic regions were identified, with large effects of the significant haplotype explaining between 4.8 and 9.8% of the phenotypic variance of the studied traits. One major QTL was identified to overlap between gel strength and yield stress, the QTL identified with the most significant SNP closest to the gene coding for κ-casein (CSN3). In addition, a chromosome-wide significant region affecting yield stress on BTA 11 was identified to be colocated with PAEP, coding for β-lactoglobulin. Furthermore, the coagulation properties of the genetic variants within the 2 genes were compared with the coagulation properties identified by the patterns of the haplotypes within the regions, and it was discovered that the haplotypes were more diverse and in one case slightly better at explaining the phenotypic variance. Besides these significant QTL comprising the 2 milk proteins, 3 additional genes are proposed as possible candidates, namely RAB22A, CDH13, and STAT1, and all have previously been found to be expressed in the mammary gland. To our knowledge, this is the first attempt to map QTL regions for acid-induced coagulation properties.

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“…PRLR is one of the main proteins of bovine milk (Ganai et al, 2009 showed the highest milk yield, while GG genotype cows showed the highest fat content (Shi et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Polymorphisms of LEP, LGB and PRLR in water buffalo

Seong¹,

Kong²

2012

Korean Journal of Agricultural Science

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:The polymorphisms of several genes including Leptin (LEP), beta-lactoglobulin (LGB) and Prolactin receptor (PRLR) have been shown to affect milk composition traits in dairy cattle. But, the effects of these polymorphisms on the milk traits of Philippine water buffalo are still unclear. In the Philippines, buffalo are the major milk producers most of which are the Philippine carabao (PC), the American Murrah Buffalo (AMB) and Bulgarian Murrah Buffalo (BMB). The LEP, LGB and PRLR genes are considered to be associated with milk production traits. The objective of the present study was to identify the single nucleotide polymorphisms (SNPs) in the LEP, LGB and PRLR genes of PC, AMB and BMB and to investigate the effect of the SNPs on milk production traits in these buffalo. Genetic polymorphisms were screened by DNA sequencing and 12 SNPs were detected in BMB; 5 SNPs were in LEP exon3 region (G14227A, G14343A, T14502C, C14526T, G14603A); 5 SNPs were in LGB exon 2 region (G1861C, A1900G, G1901T, T1948C, G1949A); 2 SNPs were in PRLR exon 6 (T59047C, T59109C). Also, 12 polymorphism sites between cattle and buffalo were identified. Our analysis of the association between SNPs and milk production traits should be useful in future studies of buffalo breeding to improve lactation performance.

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Novel polymorphisms in the bovine β‐lactoglobulin gene and their effects on β‐lactoglobulin protein concentration in milk

Cited by 57 publications

References 19 publications

Study of polymorphisms in the promoter region of ovine β-lactoglobulin gene and phylogenetic analysis among the Valle del Belice breed and other sheep breeds considered as ancestors

Study of polymorphisms in the promoter region of ovine β-lactoglobulin gene and phylogenetic analysis among the Valle del Belice breed and other sheep breeds considered as ancestors

Bovine chromosomal regions affecting rheological traits in acid-induced skim milk gels

Polymorphisms of LEP, LGB and PRLR in water buffalo

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