Novel polymorphisms of the IGF1R gene and their association with average daily gain in Egyptian buffalo (Bubalus bubalis)

El-Magd, Mohammed A.; Abbas, Hala; Elkattawy, Azza M.; Mokhbatly, Abd Allah Ahmed

doi:10.1016/j.domaniend.2013.06.004

Cited by 34 publications

(28 citation statements)

References 21 publications

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“…As a main receptor of IGFs, IGF1R mediates the transduction of metabolic signals of cell proliferation, bone growth, and protein synthesis in the GH/IGF pathway [31]. In our study, IGF1R was down-regulated in VE treatment, and because of IGF1R polymorphisms have been associated to growth traits [32–36], we thought that this effect could be due to the higher ADG in CON animals. However, ADG values were included as a covariate in the statistical model used to validate expression differences by qPCR, thus avoiding estimation biases related to ADG.…”

Section: Discussionmentioning

confidence: 66%

Genome-wide expression profiling in muscle and subcutaneous fat of lambs in response to the intake of concentrate supplemented with vitamin E

et al. 2017

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BackgroundThe objective of this study was to acquire a broader, more comprehensive picture of the transcriptional changes in the L. Thoracis muscle (LT) and subcutaneous fat (SF) of lambs supplemented with vitamin E. Furthermore, we aimed to identify novel genes involved in the metabolism of vitamin E that might also be involved in meat quality. In the first treatment, seven lambs were fed a basal concentrate from weaning to slaughter (CON). In the second treatment, seven lambs received basal concentrate from weaning to 4.71 ± 2.62 days and thereafter concentrate supplemented with 500 mg dl-α-tocopheryl acetate/kg (VE) during the last 33.28 ± 1.07 days before slaughter.ResultsThe addition of vitamin E to the diet increased the α-tocopherol muscle content and drastically diminished the lipid oxidation of meat. Gene expression profiles for treatments VE and CON were clearly separated from each other in the LT and SF. Vitamin E supplementation had a dramatic effect on subcutaneous fat gene expression, showing general up-regulation of significant genes, compared to CON treatment. In LT, vitamin E supplementation caused down-regulation of genes related to intracellular signaling cascade. Functional analysis of SF showed that vitamin E supplementation caused up-regulation of the lipid biosynthesis process, cholesterol, and sterol and steroid biosynthesis, and it down-regulated genes related to the stress response.ConclusionsDifferent gene expression patterns were found between the SF and LT, suggesting tissue specific responses to vitamin E supplementation. Our study enabled us to identify novel genes and metabolic pathways related to vitamin E metabolism that might be implicated in meat quality. Further exploration of these genes and vitamin E could lead to a better understanding of how vitamin E affects the oxidative process that occurs in manufactured meat products.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3405-8) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 66%

Genome-wide expression profiling in muscle and subcutaneous fat of lambs in response to the intake of concentrate supplemented with vitamin E

et al. 2017

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“…Polymorphism in the IGF-1 gene was observed to be associated with postpartum resumption of ovarian cyclicity in Holstein cows (Nicolini et al, 2013). In a genetic polymorphism study of the IGF-I gene, three SNPs were detected in three buffalo breeds of Gujarat (Fatima et al, 2009) and six and five novel polymorphisms in Egyptian water buffalo and Indian buffalo, respectively, in the IGF-1 receptor gene and these SNPs were associated with growth traits (El-Magd et al, 2013). Khederzadeh & Yazdanpanah (2013) reported a low variability in the exon 1 region, while observing a high degree of genetic diversity in the 5´ flanking region of the IGF-1 gene in southern populations of Iranian Buffalo.…”

Section: Discussionmentioning

confidence: 99%

“…Eleven novel polymorphisms were also observed earlier for buffalo, that is, six in Egyptian water buffalo and five in Indian buffalo in the IGF-1 receptor. These SNPs were also associated with growth traits (El-Magd et al, 2013). Khederzadeh & Yazdanpanah (2013) showed a low variability in the exon 1 region while observing a high degree of genetic diversity in the 5´ Flanking Region of the IGF-1 gene in southern populations of Iranian buffalo.…”

Section: Introductionmentioning

confidence: 94%

Genetic variability of bovine GHR, IGF-1 and IGFBP-3 genes in Indian cattle and buffalo

Ramesha¹,

Rao²,

Basavaraju³

et al. 2016

SA J. An. Sci.

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The identification of genetic polymorphisms in the genes that play a crucial role in regulatiing growth and development of livestock enables us to evaluate the biological similarities and to acquire a better perspective of quantitative traits. The present study was undertaken to characterize genetic variability in the bovine growth hormone receptor (GHR), insulin-like growth factor 1 (IGF-1) and insulin-like growth factor binding protein 3 (IGFBP-3) genes among Bos indicus (Malnad Gidda, Khillar), Bos taurus (Holstein Friesian, Jersey) cattle and Asian water buffalo Bubalus bubalis (Murrah, Surti) using polymerase chain reactionsingle strand conformation polymorphism (PCR-SSCP) analysis. These polymorphisms were confirmed by direct sequencing. The comparative gene sequence analysis in cattle and buffalo breeds revealed 18 single nucleotide polymorphisms (SNPs) across different loci. Eight SNPs were detected in the bovine growth hormone receptor (GHR) gene, of which four were found in the promoter region and four in the exon 4 region. In the IGF-1 gene, two SNPs were observed in the 5ˈUTR, three SNPs in the intron 3 region and two SNPs in the coding region of exon 4. Three SNPs were detected in the exon 2 region of the bovine IGFBP-3 gene. The frequency of rare alleles observed in the present study ranged from 0.04 to 0.16. The present results revealed high levels of genetic variability in the GHR, IGF-1 and IGFBP-3 genes in cattle and buffalo reared in India.

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“…Total RNA was isolated from mice intestine using Gene JET RNA Purification Kit (# K0731; Thermo Scientific, USA) according to the manufacturer's instructions . Total RNA (5 μg) was reverse transcribed using RevertAid H Minus Reverse Transcriptase (#EP0451; Thermo Scientific, Waltham, MA, USA) to produce cDNA.…”

Section: Methodsmentioning

confidence: 99%

Immunological interaction between Giardia cyst extract and experimental toxoplasmosis

Ashour

Saad

Dawood

et al. 2017

Parasite Immunology

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SummaryToxoplasmosis is mostly associated with other intestinal parasitic infections especially Giardia due to shared mode of peroral infection. Toxoplasma and Giardia induce a strong T-helper 1-immune response. Our aim was to induce a protective immune response that results in significant impact on intestinal and extra-intestinal phases of Toxoplasma infection. This study was conducted in experimental animals and assessment of Giardia cyst extract effect on Toxoplasma infection was investigated by histopathological examination of small intestine and brain, Toxoplasma cyst count and iNOS staining of the brain, measurement of IFN-γ and TGF-β in intestinal tissues. Results showed that the brain Toxoplasma cyst number was decreased in mice infected with Toxoplasma then received Giardia cyst extract as compared to mice infected with Toxoplasma only. This effect was produced because Giardia cyst extract augmented the immune response to Toxoplasma infection as evidenced by severe inflammatory reaction in the intestinal and brain tissues, increased levels of IFN-γ and TGF-β in intestinal tissues and strong iNOS staining of the brain. In conclusion, Giardia cyst extract generated a protective response against T. gondii infection. Therefore, Giardia antigen will be a suitable candidate for further researches as an immunomodulatory agent against Toxoplasma infection. K E Y W O R D SGiardia, IFN-γ, iNOS, TGF-β, Toxoplasma

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Novel polymorphisms of the IGF1R gene and their association with average daily gain in Egyptian buffalo (Bubalus bubalis)

Cited by 34 publications

References 21 publications

Genome-wide expression profiling in muscle and subcutaneous fat of lambs in response to the intake of concentrate supplemented with vitamin E

Genome-wide expression profiling in muscle and subcutaneous fat of lambs in response to the intake of concentrate supplemented with vitamin E

Genetic variability of bovine GHR, IGF-1 and IGFBP-3 genes in Indian cattle and buffalo

Immunological interaction between Giardia cyst extract and experimental toxoplasmosis

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