Novel Porcine Models of Myocardial Ischemia/Infarction – Technical Progress, Modified Electrocardiograms Validating, and Future Application

Liu, Jianxun; Li, Xinzhi

doi:10.5772/23818

Cited by 2 publications

(5 citation statements)

References 41 publications

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“…All images were acquired under apnoea with continuous ECG gating. Left ventricular (LV) global function was assessed at baseline and at sacrifice, as previously described [ 6 ], and the ejection fraction (LVEF), end systolic volume (LVESV), end diastolic volume (LVEDV), and cardiac output (CO) were measured and compared between groups.…”

Section: Methodsmentioning

confidence: 99%

“…After 10% buffered formalin fixation, 5 μm tissue sections were stained using Masson’s and Gallego’s trichrome procedures for histological examination. In addition, Picrosirius red staining was performed to analyse myocardial fibrosis, as previously described [ 6 ]. The collagen volume fraction (CVF), collagen I, collagen III, and collagen I/III ratio were measured in the infarct core and remote zones with Image-Pro Plus software.…”

Section: Methodsmentioning

confidence: 99%

“…Numerous animal models evaluate the pathophysiological mechanisms of ventricular remodeling and MI development and progression [ 4 , 5 ]. Briefly, swine are mostly used because of their high similarity to humans, as they share minimal pre-existing coronary collaterals and have a similar coronary physiology and anatomy [ 6 ]. Despite acceleration of early myocardial healing process in swine compared to humans [ 7 ], histopathologic changes after MI are common with an earliest myocardial necrosis followed by infiltration of myofibroblast and inflammatory cells (i.e.…”

Section: Introductionmentioning

confidence: 99%

“…neutrophils, macrophages, lymphocytes and plasma cells), and necrotic tissue replacement by collagen I and III [ 8 ]. MI pig model by permanent arterial occlusion can be induced following intracoronary ethanol administration [ 9 ], cryo-injury, cauterization [ 6 ], surgical ligation [ 10 ], and coil embolization [ 11 ]. Although ethanol, cryo-injury and cauterization are feasible and easily reproducible, these techniques promote pathophysiological changes different than those observed in humans [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…MI pig model by permanent arterial occlusion can be induced following intracoronary ethanol administration [ 9 ], cryo-injury, cauterization [ 6 ], surgical ligation [ 10 ], and coil embolization [ 11 ]. Although ethanol, cryo-injury and cauterization are feasible and easily reproducible, these techniques promote pathophysiological changes different than those observed in humans [ 6 ]. Surgical coronary ligation causes irreversible damage to the myocardium and has been used extensively to assess cardiac regeneration following cell-based therapy and tissue engineering approaches.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of two preclinical myocardial infarct models: coronary coil deployment versus surgical ligation

et al. 2014

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BackgroundDespite recent advances, myocardial infarction (MI) remains the leading cause of death worldwide. Pre-clinical animal models that closely mimic human MI are pivotal for a quick translation of research and swine have similarities in anatomy and physiology. Here, we compared coronary surgical ligation versus coil embolization MI models in swine.MethodsFifteen animals were randomly distributed to undergo surgical ligation (n = 7) or coil embolization (n = 8). We evaluated infarct size, scar fibrosis, inflammation, myocardial vascularization, and cardiac function by magnetic resonance imaging (MRI).ResultsThirty-five days after MI, there were no differences between the models in infarct size (P = 0.53), left ventricular (LV) ejection fraction (P = 0.19), LV end systolic volume (P = 0.22), LV end diastolic volume (P = 0.84), and cardiac output (P = 0.89). Histologically, cardiac scars did not differ and the collagen content, collagen type I (I), collagen type III (III), and the I/III ratio were similar in both groups. Inflammation was assessed using specific anti-CD3 and anti-CD25 antibodies. There was similar activation of inflammation throughout the heart after coil embolization (P = 0.78); while, there were more activated lymphocytes in the infarcted myocardium in the surgical occlusion model (P = 0.02). Less myocardial vascularization in the infarction areas compared with the border and remote zones only in coil embolization animals was observed (P = 0.004 and P = 0.014, respectively).ConclusionsOur results support that surgical occlusion and coil embolization MI models generate similar infarct size, cardiac function impairment, and myocardial fibrosis; although, inflammation and myocardial vascularization levels were closer to those found in humans when coil embolization was performed.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Comparison of two preclinical myocardial infarct models: coronary coil deployment versus surgical ligation

et al. 2014

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Experimental Animal Models in Heart Disease

Gök,

Beyazçiçek

2024

Düzce Tıp Fakültesi Dergisi

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Heart diseases constitute a significant global burden of mortality and morbidity. This encompassing word refers to a variety of illnesses, including coronary artery disease, heart failure, myocardial infarction, and valvular heart disease. Given the imperative need to comprehend and address these ailments, experimental studies are indispensable. Experimental animal models serve as indispensable tools in elucidating the mechanisms of heart disease. They are pivotal for developing novel treatments and assessing the efficacy of existing therapies. Among the commonly utilized animal models in heart disease research are mice, rats, rabbits, dogs, and pigs. Each model offers distinct advantages and limitations, allowing researchers to probe specific facets of cardiac pathology and unravel the intricate mechanisms involved in heart disease. In this comprehensive review, it was aimed to provide a succinct overview of the various animal models employed in heart disease research. The advantages and drawbacks of each model were delineated, the aspects of human heart disease they emulate were elucidated, and pivotal research findings facilitated by their utilization were highlighted. By synthesizing this information, it was the endeavor to provide researchers and clinicians with valuable insights into the diverse array of animal models available for investigating heart diseases, ultimately paving the way for enhanced understanding and treatment of these debilitating conditions.

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Novel Porcine Models of Myocardial Ischemia/Infarction – Technical Progress, Modified Electrocardiograms Validating, and Future Application

Cited by 2 publications

References 41 publications

Comparison of two preclinical myocardial infarct models: coronary coil deployment versus surgical ligation

Comparison of two preclinical myocardial infarct models: coronary coil deployment versus surgical ligation

Experimental Animal Models in Heart Disease

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