Novel probiotic candidates for humans isolated from raw fruits and vegetables

Vitali, Beatrice; Minervini, Giovanna; Rizzello, Carlo Giuseppe; Spisni, Enzo; Maccaferri, Simone; Brigidi, Patrizia; Gobbetti, Marco; Cagno, Raffaella Di

doi:10.1016/j.fm.2011.12.027

Cited by 101 publications

(64 citation statements)

References 53 publications

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“…Almost similar inhibitory spectra were found against the full range of indicator microorganisms. All the strains in this study were previously shown to exert antimicrobial activity toward human pathogenic bacteria (36). The antimicrobial activity of L. plantarum C2 was the most intense.…”

Section: Discussionmentioning

confidence: 75%

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Quorum-Sensing Regulation of Constitutive Plantaricin by Lactobacillus plantarum Strains under a Model System for Vegetables and Fruits

Rizzello

Filannino

Cagno

et al. 2014

Appl Environ Microbiol

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This study aimed at investigating the regulatory system of bacteriocin synthesis by Lactobacillus plantarum strains in vegetables and fruits in a model system. Sterile and neutralized cell-free supernatant (CFS) from L. plantarum strains grown in MRS broth showed in vitro antimicrobial activities toward various indicator strains. The highest activity was that of L. plantarum C2. The antimicrobial activity was further assayed on vegetable and fruit agar plates (solid conditions) and in juices (liquid conditions). A regulatory mechanism of bacteriocin synthesis via quorum sensing was hypothesized. The synthesis of antimicrobial compounds seemed to be constitutive under solid conditions of growth on vegetable and fruit agar plates. In contrast, it depended on the size of the inoculum when L. plantarum C2 was grown in carrot juice. Only the inoculum of ca. 9.0 log CFU ml ؊1 produced detectable activity. The genes plnA, plnEF, plnG, and plnH were found in all L. plantarum strains. The genes plnJK and plnN were detected in only three or four strains. Reverse-phase high-performance liquid chromatography purification and mass spectrometry analysis revealed the presence of a mixture of eight peptides in the most active fraction of the CFS from L. plantarum C2. Active peptides were encrypted into bacteriocin precursors, such as plantaricins PlnJ/K and PlnH and PlnG, which are involved in the ABC transport system. A real-time PCR assay showed an increase in the expression of plnJK and plnG during growth of L. plantarum C2 in carrot juice.

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Section: Discussionmentioning

confidence: 75%

“…Six strains of Lactobacillus plantarum were assayed for antimicrobial activity (Table 1). Previously, all strains had been selected as potential probiotic candidates (36). Propagation was routinely carried out in MRS broth (Oxoid) at 30°C for 24 h under anaerobiosis.…”

Section: Methodsmentioning

confidence: 99%

Quorum-Sensing Regulation of Constitutive Plantaricin by Lactobacillus plantarum Strains under a Model System for Vegetables and Fruits

Rizzello

Filannino

Cagno

et al. 2014

Appl Environ Microbiol

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“…Current evidence for the efficacy of probiotics in miscellaneous illnesses (mainly intestinal disorders) is inconsistent however, and is rendered more complex by the fact that treatment regimens across different studies are highly heterogeneous, with different species/ strains, dosages, lengths of treatment and administration methods employed. Probiotics are also found in food intake: an estimated 35% of all lactic acid bacteria isolated from raw fruits and vegetables can survive gastric conditions [88].…”

Section: Probioticsmentioning

confidence: 99%

The “psychomicrobiotic”: Targeting microbiota in major psychiatric disorders: A systematic review

Fond

Boukouaci

Chevalier

et al. 2015

Pathologie Biologie

185

127

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“…Enterohemorragic Escherichia coli DSM 30083 (DSMZ, Leibniz Institute German Collection of Microorganisms and Cell Cultures, Germany) and Bacillus megaterium F6 (Vitali et al, 2012) were used as indicator strains. E. coli DSM 30083 and B. megaterium F6 were grown on Luria Bertani (LB, Oxoid) medium for 24 h at 37 and 30 C, respectively.…”

Section: Antimicrobial Assaymentioning

confidence: 99%

Exploitation of Leuconostoc mesenteroides strains to improve shelf life, rheological, sensory and functional features of prickly pear (Opuntia ficus-indica L.) fruit puree

et al. 2016

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Novel probiotic candidates for humans isolated from raw fruits and vegetables

Cited by 101 publications

References 53 publications

Quorum-Sensing Regulation of Constitutive Plantaricin by Lactobacillus plantarum Strains under a Model System for Vegetables and Fruits

Quorum-Sensing Regulation of Constitutive Plantaricin by Lactobacillus plantarum Strains under a Model System for Vegetables and Fruits

The “psychomicrobiotic”: Targeting microbiota in major psychiatric disorders: A systematic review

Exploitation of Leuconostoc mesenteroides strains to improve shelf life, rheological, sensory and functional features of prickly pear (Opuntia ficus-indica L.) fruit puree

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