Novel Probiotic Isolation of Coconut Water's Helpful Lactic Acid Bacteria Cure Covid-19 Patients

Abstract

Probiotics from coconut water lactic acid bacteria have not isolated nor identified them, and no one has studied that these probiotics can help cure Covid-19 patients. This study aimed to isolate and identify probiotic lactic acid bacteria in coconut water, both morphologically, physiologically, and biochemically. Further studied his ability to help cure Covid-19 patients. Using coconut water samples, probiotics are isolated by isolating lactic acid bacteria through dilution methods and pour plate methods. The medium used is MRSA+ CaCO3 0.3%. Physiological and morphological identification. The following identification is to perform biochemical tests such as the Gram test. The results showed that there were 73 bacterial isolates and 24 fungal isolates, consisting of 4 species of bacteria, Pediococcus sp, Lactobacillus paracasei, and Lactobacillus plantarum, as well as candida sp and rhizopus fungi. In comparison, the results of the antimicrobial analysis showed that Lactobacillus plantarum has better antimicrobial ability against the pathogen bacteria Pseudomonas aureginosa. Lactobacillus plantarum's antimicrobial ability is less good against S. aureus bacteria. These findings suggest that probiotics from coconut water can be isolated and may aid the healing of Covid-19 patients. This study has implications that in the future Covid-19 patients can be helped with coconut water.

Effect of Coconut Water Storage Time and Inoculum Size of Lentilactobacillus parafarraginis on Dried Bacterial Cellulose Properties

“…41 The quality of coconut water changed during storage due to the fermentation process by natural microorganisms, such as L. plantarum, L. paracasei, and Pediococcus sp. 20 This contributed to an enhancement in the quantity of natural bacteria, mainly lactic acid bacteria and a reduction in the pH of coconut water. 21 Therefore, the longer the storage time of coconut water, the more acidic the pH of bacterial cellulose.…”

“…17 Unfortunately, coconut water quality was altered during storage due to the fermentation process by natural microorganisms, such as L. paracasei, L. plantarum, and Pediococcus sp. 20 This resulted in an increase of lactic acid bacteria and a reduction of the pH of coconut water. 21 Furthermore, the natural fermentation process altered the composition of coconut water, mainly sugar, the optimum carbon source for microbial growth.…”

Anti-inflammatory and Immunostimulant Therapy with Lactobacillus fermentum and Lactobacillus plantarum in COVID-19: A Literature Review

“…The metabolites produced by lactic acid bacteria can inhibit the development of pathogenic bacteria and viruses 73 . These metabolites include amino acid derivatives (indolelactic acid, phenyllactic acid 2-hydroxy-4-,2-hydroxy-4-methylpentanoic acid, and 2-hydroxy-4-methylthio butanoic acid), fatty acids (3-hydroxy-5-cis-dodecanoic acid and 3-hydroxydodecanoic acid), organic compounds (acetic acid, lactic acid, propionic acid, succinic acid, and benzoate acid), cyclic peptides (cyclo(L-Phe-L-Pro) reutericyclin), and other groups of chemical compounds (δ-dodecalactone) 74 .…”