Novel prognostic marker LINC00205 promotes tumorigenesis and metastasis by competitively suppressing miRNA-26a in gastric cancer

Abstract

Rapid proliferation and metastasis of gastric cancer (GC) resulted in a poor prognosis in the clinic. Previous studies elucidated that long non-coding RNA (LncRNA) LINC00205 was upregulated in various tumors and participated in tumor progression. The aim of our study was to investigate the regulating role of LINC00205 in tumorigenesis and metastasis of GC. Both public datasets and our data showed that the LINC00205 was highly expressed in GC tissues and several cell lines. Notably, GC patients with high level of LINC00205 had a poor prognosis in our cohort. Mechanistically, knockdown of LINC00205 by shRNAs suppressed GC cells proliferation, migration, invasion remarkably, and induced cell cycle arrest. Based on bioinformatics prediction, we found that LINC00205 might act as a competitive endogenous RNA (ceRNA) through targeting miR-26a. The level of miR-26a had negatively correlated with LINC00205 expression and was decreased among GC cell lines, tissues, and serum samples. Our results for the first time confirmed that miR-26a was a direct target of LINC00205 and might have the potential to become a plasma marker for clinical tumor diagnosis. Indeed, LINC00205 knockdown resulted in the dramatic promotion of miR-26a expression as well as inhibition of miR-26a potential downstream targets, such as HMGA2, EZH2, and USP15. These targets were essential for cell survival and epithelial-mesenchymal transition. Importantly, LINC00205 was able to remodel the miR-26a-mediated downstream silence, which identified a new mechanism of malignant transformation of GC cells. In conclusion, this study revealed the regulating role of the LINC00205/miR-26a axis in GC progression and provided a new potential therapeutic strategy for GC treatment.

Cuproptosis-related lncRNA predict prognosis and immune response of lung adenocarcinoma

“…Li et al found that LINC00205 targets the oncogene miR-185-5p as a potential therapeutic target in lung and gastric cancers. LINC00205 promoted the proliferation and migration of gastric cancer cells by inhibiting miR-26a [ 36 ]. Zhang et al suggested that LINC00205 targeting the high mobility group box 1 protein promoted the proliferation of neuroblastoma, which was correlated with OS [ 34 ].…”

“…In hepatocellular carcinoma, LINC00592 was associated with patient prognosis and could target the cold shock domain-containing E1 protein, CDK6, miR-122-5p, and epoxide hydrolase 1, promoting the proliferation of hepatocellular carcinoma cells [30][31][32][33]. Li the proliferation and migration of gastric cancer cells by inhibiting miR-26a [36]. Zhang et al suggested that LINC00205 targeting the high mobility group box 1 protein promoted the proliferation of neuroblastoma, which was correlated with OS [34].…”

A prognostic signature based on the expression profile of the ferroptosis-related long non-coding RNAs in hepatocellular carcinoma

“…Notably, the biological functions of some of the identified lncRNAs (LINC00205, ZFPM2-AS1, LINC00942) have also been associated with other malignancies and might thus be nonspecific to HCC. [22][23][24][27][28][29][30]33,34 However, numerous other lncRNAs (AL139384.1, AL928654.1, AC145207.8, POLH-AS1, AC090772.3, AL603839.3, AC012073.1, AC099850.1, AC026401.3, AP001469.3, AL031985.3, SNHG10, SNHG21) lack corresponding basic studies to validate their prognostic roles in the development of HCC. Further studies are therefore needed to establish the associations of these identified lncRNAs with ferroptosis and their contribution to the regulation of HCC pathogenesis through ferroptosis.…”

“…• enhances hepatoblastoma progression by regulating ROCK1 expression via sponging miR-154-3p through MAPK signaling 22 ; • promotes tumorigenesis and metastasis by competitively suppressing miRNA-26a in gastric cancer 23 ; • facilitates malignant phenotypes in lung cancer by recruiting FUS to stabilize CSDE1. 24 • activated by YY-1, facilitates the proliferation of HCC cells through YY1/miR-26a-5p/CDK6 25 ; • enhances tumorigenicity of HCC cells through modulating EPHX1 and inhibiting miR-184.…”

Construction and Validation of Pyroptosis-Related lncRNA Prediction Model for Colon Adenocarcinoma and Immune Infiltration Analysis

“…The same characteristics also occurred in LINC00205. It may be used as a novel prognostic indicator of several cancers in multiple bioinformatics analyses, such as glioma [31], gastric cancer [32], and hepatocellular carcinoma [33][34][35]. Multiple lines of experimental evidence from basic research demonstrated the oncogenic role of LINC00205 in HCC, which may be beneficial for diagnosing and treating HCC [34,36].…”