2010
DOI: 10.1111/j.1600-0854.2010.01057.x
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Novel PSI Domains in Plant and Animal H+-Inositol Symporters

Abstract: Uptake of carbohydrates across plasma membranes of animal cells is mediated mainly by Na + -driven transporters, such as the sodium-dependent glucose transporter(SGLT) (1) or the sodium-myo-inositol co-transporter (SMIT) (2). In contrast, animal monosaccharide transporters of the GLUT family (GLUT1 to GLUT12) are energy-independent (1). One protein of this family, however, GLUT13, acts as energy-dependent H + -inositol symporter (HMIT) (3). All 13 GLUT proteins are closely related to H + -dependent sugar trans… Show more

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Cited by 16 publications
(16 citation statements)
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“…They are absent from the tonoplastlocalized plant paralogs. Removal of the PSI domain neither affected the transport activity nor the sorting to the plasma membrane (Dotzauer et al, 2010).…”
Section: A Di-leu Motif Targets Int1 To the Tonoplastmentioning
confidence: 82%
See 1 more Smart Citation
“…They are absent from the tonoplastlocalized plant paralogs. Removal of the PSI domain neither affected the transport activity nor the sorting to the plasma membrane (Dotzauer et al, 2010).…”
Section: A Di-leu Motif Targets Int1 To the Tonoplastmentioning
confidence: 82%
“…Insertion into the vector pCS120 (Dotzauer et al, 2010) All chimera, truncations, and mutations of INT1 and INT4 contain flanking NcoI or PciI cloning sites for insertion into the expression vectors. For GFP fusion constructs, the above-described constructs were excised from pJET1.2 with NcoI or PciI and inserted into the unique NcoI cloning sites of pCS120 (Dotzauer et al, 2010) for C-terminal GFP fusions or pSS87 for N-terminal GFP fusions.…”
Section: Constructs For Transient and Stable Transformationmentioning
confidence: 99%
“…The full‐length coding sequence of RGS1 was amplified using primers RGS1–5‐NcoI (5′‐CCATGGCGAGTGGATGTGCTCTACATGGTGGTTG‐3′) and RGS1–3‐NcoI (5′‐CCATGGCACCGGGACTACTGCATCTGGAACTCTTTGAC‐3′). The resulting sequences were then cloned into protoplast expression vector pCS120 for C–terminal GFP fusions (Dotzauer et al ., ) or protoplast expression vector pSS87 for N–terminal GFP fusions (Schneider et al ., ). As a PM marker for co‐localization studies, we used the myo ‐inositol transporter AtINT4 (At4g16480) C–terminally fused to RFP (Wolfenstetter et al ., ).…”
Section: Methodsmentioning
confidence: 99%
“…Conserved cysteins within the enlarged extracellular loop that are responsible for formation of the PSI domain are highlighted in orange. Protein structures modified after Dotzauer et al [22].…”
Section: Proton Coupled Inositol Transportersmentioning
confidence: 99%