Novel Purification Method for Recombinant 3AB1 Nonstructural Protein of Foot-and-Mouth Disease Virus for Use in Differentiation between Infected and Vaccinated Animals

Nanni, Mariana; Alegre, Mariana; Compaired, Diego; Taboga, Oscar; Fondevila, Norberto

doi:10.1177/104063870501700306

Cited by 8 publications

(3 citation statements)

References 10 publications

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“…Antigen-Based Diagnosis. The 3AB protein of FMDV was expressed in E. coli [185][186][187] or in P. pastoris [188] and has been used for the diagnosis of FMD infection in cattle. Similarly, 3ABC proteins expressed in heterologous systems were used in ELISA (3ABC ELISA) for serodiagnosis of FMD [189,190].…”

Section: Recombinantmentioning

confidence: 99%

A Brief Review on Diagnosis of Foot-and-Mouth Disease of Livestock: Conventional to Molecular Tools

Longjam

Deb

Sarmah

et al. 2011

Veterinary Medicine International

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Foot-and-mouth disease (FMD) is one of the highly contagious diseases of domestic animals. Effective control of this disease needs sensitive, specific, and quick diagnostic tools at each tier of control strategy. In this paper we have outlined various diagnostic approaches from old to new generation in a nutshell. Presently FMD diagnosis is being carried out using techniques such as Virus Isolation (VI), Sandwich-ELISA (S-ELISA), Liquid-Phase Blocking ELISA (LPBE), Multiplex-PCR (m-PCR), and indirect ELISA (DIVA), and real time-PCR can be used for detection of antibody against nonstructural proteins. Nucleotide sequencing for serotyping, microarray as well as recombinant antigen-based detection, biosensor, phage display, and nucleic-acid-based diagnostic are on the way for rapid and specific detection of FMDV. Various pen side tests, namely, lateral flow, RT-LAMP, Immunostrip tests, and so forth. are also developed for detection of the virus in field condition.

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Section: Recombinantmentioning

confidence: 99%

A Brief Review on Diagnosis of Foot-and-Mouth Disease of Livestock: Conventional to Molecular Tools

Longjam

Deb

Sarmah

et al. 2011

Veterinary Medicine International

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“…Expression yielded approximately 14 mg of affinity-purified MBP-c3B antigen per 100 ml of culture, i.e., sufficient antigen to coat approximately 2,800 plates with 96 wells, at 50 ng/well. Others have reported yields for 3AB and 3B proteins of 3 and 1 mg/100 ml culture, respectively (44,54). Coomassie blue staining of MBP-c3B revealed a band at ϳ52 kDa, consistent with its predicted molecular mass.…”

Section: Discussionmentioning

confidence: 61%

Pan-Serotype Diagnostic for Foot-and-Mouth Disease Using the Consensus Antigen of Nonstructural Protein 3B

et al. 2015

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bAn amino acid consensus sequence for the seven serotypes of foot-and-mouth disease virus (FMDV) nonstructural protein 3B, including all three contiguous repeats, and its use in the development of a pan-serotype diagnostic test for all seven FMDV serotypes are described. The amino acid consensus sequence of the 3B protein was determined from a multiple-sequence alignment of 125 sequences of 3B. The consensus 3B (c3B) protein was expressed as a soluble recombinant fusion protein with maltosebinding protein (MBP) using a bacterial expression system and was affinity purified using amylose resin. The MBP-c3B protein was used as the antigen in the development of a competition enzyme-linked immunosorbent assay (cELISA) for detection of anti-3B antibodies in bovine sera. The comparative diagnostic sensitivity and specificity at 47% inhibition were estimated to be 87.22% and 93.15%, respectively. Reactivity of c3B with bovine sera representing the seven FMDV serotypes demonstrated the pan-serotype diagnostic capability of this bioreagent. The consensus antigen and competition ELISA are described here as candidates for a pan-serotype diagnostic test for FMDV infection. Foot-and-mouth disease (FMD) is the most contagious viral vesicular disease that affects cloven-hoofed livestock species. FMD has significant global socioeconomic consequences, from national livestock industries suffering because of international trade restrictions to subsistence farmers suffering because of losses of stock productivity and livelihoods. Western European, North American, and Far East Asian/Pacific regions and most South American countries have official recognition of freedom-from-FMD status, with or without vaccination, by the World Organization for Animal Health (OIE). Regions in which FMD remains endemic tend to be those of lesser economic capacity, and this limits their ability to control or to eradicate the disease (1, 2). As a result, FMD remains an ongoing problem in regions in which it is endemic, and it is a persistent threat to regions that are free of the disease. Seven serotypes have been described for foot-and-mouth disease virus (FMDV), i.e., O, A, C, Asia 1, and South African Territories 1 (SAT1), SAT2, and SAT3. The disease forms produced by each serotype are clinically indistinguishable (3); similarly, FMD is clinically indistinguishable from other vesicular lesion-causing diseases, such as vesicular stomatitis, swine vesicular exanthema, and swine vesicular disease, and laboratory-based clinical diagnosis is required (4, 5). FMD infections may be mild or subclinical in ovine or caprine species, further complicating clinical diagnosis (6, 7). Exposure to one serotype does not confer cross-serotype immunity, potentially complicating diagnosis when multiple serotypes are circulating during an outbreak (8). A definitive diagnosis of FMD is possible only with laboratory testing.Conventional serological assays for FMD, including the virus neutralization test, liquid-phase blocking enzyme-linked immunosorbent assay (ELISA), and so...

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“…В 2015 г. M. Hosamani et al предложили использовать в непрямом варианте 3АВС-ИФА белок G, конъюгированный с пероксидазой хрена. Это позволило исследовать в н-ИФА сыворотки крови от разных видов животных [39].…”

Section: нсп-ифа в надзоре за ящуромunclassified

Detection of antibodies to non-structural proteins of foot-and-mouth disease virus (review)

Yakovleva,

Scherbakov

2023

Veterinary Science Today

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Обнаружение антител к неструктурным белкам вируса ящура (обзор) РЕЗЮМЕ Ящур -высококонтагиозное вирусное заболевание парнокопытных животных, способное вызывать эпизоотии и наносить большой экономический ущерб. Болезнь остается огромной проблемой в развивающихся странах и представляет постоянную угрозу для развитых стран. Важным инструментом контроля заболевания является обнаружение антител к неструктурным белкам вируса ящура в крови восприимчивых животных. Это единственный способ, позволяющий достоверно дифференцировать вакцинированных животных от переболевших и вирусоносителей. Для выявления антител к неструктурным протеинам вируса ящура разработаны различные модификации твердофазного иммуноферментного анализа. В качестве антигена в них используются рекомбинантные неструктурные белки вируса ящура, чаще всего 3АВС или 3АВ. За короткий срок иммуноферментный анализ с использованием рекомбинантных антигенов вируса ящура прошел путь от внутрилабораторного метода до общедоступных коммерческих тест-систем, большинство из которых обладают высокой диагностической специфичностью и чувствительностью. Данный метод широко применяется в надзоре за ящуром. В странах или зонах, благополучных по ящуру без вакцинации, иммуноферментный анализ, основанный на обнаружении антител к неструктурным белкам вируса ящура, применяется как основной метод серологического мониторинга и ретроспективной диагностики заболевания. В благополучных по ящуру странах и зонах с вакцинацией этот метод используется для доказательства отсутствия вирусной инфекции в вакцинированных стадах. В Южной Америке при эрадикации заболевания иммуноферментный анализ, основанный на обнаружении антител к неструктурным белкам вируса ящура, применялся для выявления инфицированных животных, а в настоящее время этот метод используется для мониторинга циркуляции возбудителя в еще неблагополучных по заболеванию странах Азии и Африки, реализующих программу прогрессивного контроля за ящуром. В России, как стране с зонами вакцинации против ящура, данный метод является обязательным инструментом надзора за заболеванием. Обзор составлен на основе анализа 65 источников.Ключевые слова: обзор, вирус ящура, неструктурные белки, иммуноферментный анализ Благодарности: Работа выполнена за счет средств ФГБУ «ВНИИЗЖ» в рамках тематики научно-исследовательских работ «Ветеринарное благополучие».

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Novel Purification Method for Recombinant 3AB1 Nonstructural Protein of Foot-and-Mouth Disease Virus for Use in Differentiation between Infected and Vaccinated Animals

Cited by 8 publications

References 10 publications

A Brief Review on Diagnosis of Foot-and-Mouth Disease of Livestock: Conventional to Molecular Tools

A Brief Review on Diagnosis of Foot-and-Mouth Disease of Livestock: Conventional to Molecular Tools

Pan-Serotype Diagnostic for Foot-and-Mouth Disease Using the Consensus Antigen of Nonstructural Protein 3B

Detection of antibodies to non-structural proteins of foot-and-mouth disease virus (review)

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