Novel pyridinecarboxaldehyde thiosemicarbazone conjugated magnetite nanoparticulates (MNPs) promote apoptosis in human lung cancer A549 cells

Habibi, Alireza; Shandiz, Seyed Ataollah Sadat; Salehzadeh, Ali; Moradi‐Shoeili, Zeinab

doi:10.1007/s00775-019-01728-4

Cited by 37 publications

(23 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some properties such as chromatin condensation and appearing fragmented nuclei were obvious in staining. The present findings are consistent with those from a study by Habibi et al [42] on nuclear fragmentation and chromatin condensation in A549 cells treated with pyridine derivative of thiosemicarbazone. Similar results were also observed in MCF‐7 cells treated with different derivatives of thiosemicarbazone in a study by Bai et al [59].…”

Section: Resultssupporting

confidence: 94%

“…The least and the most caspase‐3 activity in treated cells was 11 and 30.6%. The highest level of caspase activity in the study of Cazarnomysy et al [43] was consistent with the observed changes in the present study.‏ A 1.6‐fold increase in caspase‐3 activity was also observed in A549 cells treated with pyridine derivative of thiosemicarbazone/Fe 3 O 4 [42]. ‏…”

Section: Resultssupporting

confidence: 91%

“…In the case of P compound, a significant decrease was observed in cellular proliferation rate at concentration of 125 μM/ml, while a significant decrease in cell proliferation rate for PL compound was observed at concentration of 62.5 μM/ml. The high toxicity of Fe 3 O 4 /thiosemicarbazone compared to thiosemicarbazone alone, on A549 cancer cells was demonstrated in a study by Habibi et al [42]. The high toxicity of different pyrazole derivatives on MCF‐7, MB‐231 and MDA breast cancer cells lines has also been observed in the study by Czarnomysy et al [43].…”

Section: Resultsmentioning

confidence: 85%

See 2 more Smart Citations

Functionalisation of Fe ₃ O ₄ nanoparticles by 2‐((pyrazol‐4‐yl) methylene) hydrazinecarbothioamide enhances the apoptosis of human breast cancer MCF‐7 cells

Izadpanah

Salehzadeh

Zaefizadeh

et al. 2020

IET nanobiotechnol.

Self Cite

View full text Add to dashboard Cite

Cancer is a major cause of death. Thus, the incidence and mortality rate of cancer is globally important. Regarding vast problems caused by chemotherapy drugs, efforts have progressed to find new anti-cancer drugs. Pyrazole derivatives are known as components with anti-cancer properties. In here, Fe 3 O 4 nanoparticles were first functionalized with (3-chloropropyl) trimethoxysilane, then 2-((pyrazol-4-yl) methylene) hydrazinecarbothioamide (P) was anchored on the surface of magnetic nanoparticles (PL). The synthesized nano-compounds were characterized using Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, Zeta potential, dynamic light scattering, and energy-dispersive x-ray spectrometry analyses. The cytotoxicity effect was evaluated using MTT assay, apoptosis test by Flow cytometry, cell cycle analysis, Caspase-3 activity assay and Hoechst staining on MCF-7 cell line. The high toxicity for tumor cells and low toxicity on normal cells (MCF10A) was considered as an important feature (selectivity index, 10.9). Based on results, the IC50 for P and PL compounds were 157.80 and 131.84 μM/ml respectively. Moreover, apoptosis inducing, nuclear fragmentation, Caspase 3 activity and induction of cell rest in sub-G1 and S phases, were also observed. The inhibitory effect of PL was significantly higher than P, which could be due to the high penetrability of Fe 3 O 4 nanoparticles.

show abstract

Section: Resultssupporting

confidence: 94%

Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 85%

See 1 more Smart Citation

Functionalisation of Fe ₃ O ₄ nanoparticles by 2‐((pyrazol‐4‐yl) methylene) hydrazinecarbothioamide enhances the apoptosis of human breast cancer MCF‐7 cells

Izadpanah

Salehzadeh

Zaefizadeh

et al. 2020

IET nanobiotechnol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Moreover, the hydrodynamic size and zeta potential of CuO@Glu/TSC NPs were measured 710 nm and 27.5 mv, respectively. The proper zeta potential of the CuO@Glu/TSC NPs could provide su cient repulsive force between particles to avoid agglomeration of the NPs and provides satisfactory colloidal stability [15] (Figure 6).…”

Section: Resultsmentioning

confidence: 99%

Trigger of Apoptosis in Adenocarcinoma Gastric Cell Line (AGS) by a Complex of Thiosemicarbazone and Copper Nanoparticles

Badrooh

Shokrollahi

Javan

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Seeking novel anticancer agents with minimal side effects against gastric cancer is vitally important. Copper, as an important trace element, takes roles in different physiologic pathways. Also, there is a higher demand for copper in cancer cells than normal ones. Copper complexes containing a therapeutic ligand could be promising candidates for gastric cancer chemotherapy. In this work, copper oxide nanoparticles were synthesized, functionalized with glutamic acid (CuO@Glu) and conjugated with thiosemicarbazone (Cuo@Glu/TSC NPs). The NPs were characterized and their antiproliferative potential against AGS cancer cells was investigated using MTT, flow cytometry, Hoechst staining, and caspase 3 activation assays. The FT-IR results showed the proper binding of TSC to CuO@Glu NPs and crystallinity of the prepared NPs was confirmed by the XRD pattern. The EDX analysis confirmed the presence of Cu, N, C, O, and S elements and lack of impurities. The Hydrodynamic size and zeta potential of the Cuo@Glu/TSC NPs were 710 nm and 27.5 mv, respectively. The NPs had spherical shape and were in a size range of 20-39 nm in diameter. This work revealed that CuO@Glu/TSC NPs efficiently inhibited the proliferation of AGS cells with significantly lower IC50 value (203 µg/mL) than normal HEK293 cells (IC50=435µg/mL). Flow cytometry and Hoechst staining obviously revealed apoptosis induction among CuO@Glu/TSC treated cells, and caspase-3 activity significantly increased by 1.4 folds. This study introduced CuO@Glu/TSC as an efficient anticancer against gastric cancer cells with lower toxicity toward normal cells which could be employed for cancer treatment after further characterization.

show abstract

“…The solid was separated and washed with distilled water and ethanol and was dried in an oven (60°C) for 4 hr to afford Co(OH) 2 @Glu‐TSC nanoflakes. [ 26,29 ]…”

Section: Methodsmentioning

confidence: 99%

The Co(OH)₂@Glu‐TSC nanoflakes enhance the apoptosis in hepatoma G2 cell

Shahrokhshahi

Salehzadeh

Vaziri

et al. 2021

J Chinese Chemical Soc

Self Cite

View full text Add to dashboard Cite

The aim of the present study was to evaluate the impact of cobalt hydroxide nanoparticles conjugated with glutamic acid and thiosemicarbazides (Co(OH)2@Glu‐TSC) on Hepatoma G2 (HpG2) cancer cell lines. The effect of different concentrations of Co(OH)2@Glu‐TSC nanoparticles on the growth of Human Embryonic Kidney‐293 (HEK‐293) normal cells and HpG2 cancer cells was evaluated. The half maximal inhibitory concentration of Co(OH)2@Glu‐TSC nanoparticle on HEK‐293 and HepG2 was 701.65 μg/ml and 133.62 μg/ml, respectively. The percentage of HpG2 living cells in untreated group was significantly higher than that in Co(OH)2@Glu‐TSC‐treated group (95.5 vs. 59.5%; p < .001). Dot plots of Annexin V/PI staining showed a 15.3% early‐stage apoptosis and a 15.3% late‐stage apoptosis in Co(OH)2@Glu‐TSC‐treated cells. Untreated cancer cells exhibited 2.83% early‐stage apoptosis and a 0.23% late‐stage apoptosis. Intracellular Reactive Oxygen Species in Co(OH)2@Glu‐TSC‐treated cells (2,342) was significantly higher than that in untreated cells (2,342 vs. 707; p < .001). The mean expression of Caspase 3, maternally expressed gene 3, and P53 genes were significantly higher in Co(OH)2@Glu‐TSC‐treated cancer cells compared to untreated cancer cells. Co(OH)2@Glu‐TSC nanoparticle has a high cytotoxic effect on cancer cells which is possibly mediated by the induction of overproduction of reactive oxygen species, oxidative stress, and overexpression of apoptotic genes.

show abstract

Novel pyridinecarboxaldehyde thiosemicarbazone conjugated magnetite nanoparticulates (MNPs) promote apoptosis in human lung cancer A549 cells

Cited by 37 publications

References 54 publications

Functionalisation of Fe ₃ O ₄ nanoparticles by 2‐((pyrazol‐4‐yl) methylene) hydrazinecarbothioamide enhances the apoptosis of human breast cancer MCF‐7 cells

Functionalisation of Fe ₃ O ₄ nanoparticles by 2‐((pyrazol‐4‐yl) methylene) hydrazinecarbothioamide enhances the apoptosis of human breast cancer MCF‐7 cells

Trigger of Apoptosis in Adenocarcinoma Gastric Cell Line (AGS) by a Complex of Thiosemicarbazone and Copper Nanoparticles

The Co(OH)₂@Glu‐TSC nanoflakes enhance the apoptosis in hepatoma G2 cell

Contact Info

Product

Resources

About

Novel pyridinecarboxaldehyde thiosemicarbazone conjugated magnetite nanoparticulates (MNPs) promote apoptosis in human lung cancer A549 cells

Cited by 37 publications

References 54 publications

Functionalisation of Fe 3 O 4 nanoparticles by 2‐((pyrazol‐4‐yl) methylene) hydrazinecarbothioamide enhances the apoptosis of human breast cancer MCF‐7 cells

Functionalisation of Fe 3 O 4 nanoparticles by 2‐((pyrazol‐4‐yl) methylene) hydrazinecarbothioamide enhances the apoptosis of human breast cancer MCF‐7 cells

Trigger of Apoptosis in Adenocarcinoma Gastric Cell Line (AGS) by a Complex of Thiosemicarbazone and Copper Nanoparticles

The Co(OH)2@Glu‐TSC nanoflakes enhance the apoptosis in hepatoma G2 cell

Contact Info

Product

Resources

About

Functionalisation of Fe ₃ O ₄ nanoparticles by 2‐((pyrazol‐4‐yl) methylene) hydrazinecarbothioamide enhances the apoptosis of human breast cancer MCF‐7 cells

Functionalisation of Fe ₃ O ₄ nanoparticles by 2‐((pyrazol‐4‐yl) methylene) hydrazinecarbothioamide enhances the apoptosis of human breast cancer MCF‐7 cells

The Co(OH)₂@Glu‐TSC nanoflakes enhance the apoptosis in hepatoma G2 cell