Novel Rat Model of Ischemic Cardiomyopathy Induced by Repetitive Myocardial Ischemia/Reperfusion Injury While Conscious

Toyota, Eiji; Kawaguchi, Yukie; Ogasawara, Yasuo; Watanabe, Nozomi; Neishi, Yoji; Kawamoto, Takahiro; Okura, Hiroyuki; Yoshida, Kiyoshi

doi:10.1253/circj.71.788

Cited by 11 publications

(10 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3,4,[25][26][27][28][29] There are many heart failure models using mice and rat. 30,31 However, the amino acid sequences of cTnC are highly conserved from fish to mammals. The overall identity is 90%, not only in the 4 calcium-binding domains but also in the entire fragment.…”

Section: Discussionmentioning

confidence: 99%

Conditional Antisense-Knockdown of Zebrafish Cardiac Troponin C as a New Animal Model for Dilated Cardiomyopathy

Lin

Tsai

et al. 2009

Circ J

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Conditional Antisense-Knockdown of Zebrafish Cardiac Troponin C as a New Animal Model for Dilated Cardiomyopathy

Lin

Tsai

et al. 2009

Circ J

View full text Add to dashboard Cite

“…For example, end‐systolic LV size exhibited mild to severe (+65 to +554%) dilation. Previously, we demonstrated marked interindividual variation in the severity of cardiac dysfunction, myocardial hypoperfusion, coronary microvascular volume reduction and the development of fibrosis using an identical protocol of repetitive I/R injury in rats 17 . The mechanisms underlying the interindividual variation, which are likely related to heterogeneity of the genetic background 19,20 and/or coronary arterial branching and flow, 21 remain unclear.…”

Section: Discussionmentioning

confidence: 96%

“…Repetitive ischaemia was induced by a mini‐pneumatic coronary occluder implanted around the LAD of the rat, as described previously 16,17 . The occluder consists of a balloon, a basket sheath, a suture and a catheter.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Development of Cardiac Dysfunction Induced by Repetitive Transient Myocardial Ischaemia Is Inhibited by Edaravone in Conscious Rats

Toyota

Miyamoto

Imai

et al. 2009

Clin Exp Pharma Physio

Self Cite

View full text Add to dashboard Cite

1. In the present study, we investigated the effects of treatment with the hydroxyl radical scavenger 3-methyl-1-phenyl-2-pyrazolin-5-one (edaravone) on myocardial dysfunction induced by transient but frequent ischaemia in conscious rats. 2. Conscious male Wistar rats were subjected to repetitive ischaemia (RI; 40 s ischaemia every 20 min for 72 h). After the ninth episode of RI, edaravone (1 mg/kg, i.v., at each ischaemic event) or vehicle control (acetate buffer solution, i.v.) was administered. Dilation of the left ventricle (LV) after the eighth RI (fractional area change; %FAC(initial)) and after the final RI (%FAC(final)) was determined by comparing measurements (12 MHz echocardiogram) at these time-points with baseline LV area prior to RI. 3. In controls, %FAC(final) was correlated with %FAC(initial) (r = 0.98; P < 0.0001), making %FAC(initial) a predictor of %FAC(final). Edaravone treatment shifted the %FAC(initial)–%FAC(final) relationship downward (P < 0.0001), indicating that edaravone inhibited progression of LV dilation. In addition, %FAC(final) was correlated with myocardial generation of reactive oxygen species (ROS) in control samples (r = 0.88, P = 0.008), although both %FAC(final) and ROS were suppressed by edaravone treatment (P = 0.016). 4. We conclude that repetitive transient ischaemia in conscious rats induced development of cardiac dysfunction and that this phenomenon was inhibited by edaravone. We speculate that edaravone is a potential therapeutic agent that may interfere with the progression of cardiac dysfunction in high-risk patients with RI.

show abstract

“…To understand its pathology, and with the ultimate hope of finding a treatment, researchers have generated genetic rodent models of heart failure. 4,5 On page 1691 of this issue of the Journal, Ho et al 6 describe their attempt to established a transgenic model of idiopathic DCM in zebrafish using microinjection of tissue-specific and inducible RNA interference with cardiac troponin C (TnC).…”

Section: Article P 1691mentioning

confidence: 99%

Zebrafish With Antisense-Knockdown of Cardiac Troponin C as a Model of Hereditary Dilated Cardiomyopathy

Ohte

Miyoshi

Sane

et al. 2009

Circ J

View full text Add to dashboard Cite

Circ J 2009; 73: 1595 -1596 unctional abnormalities of cardiac troponin caused by either mutations or auto-antibodies can induce dilated cardiomyopathy (DCM), 1,2 which is characterized by left ventricular enlargement and systolic dysfunction resulting from a reduction in the myocardial force of contraction. DCM causes congestive heart failure, which can be treated with diuretics, angiotensin-converting enzyme inhibitors (or angiotensin type-I receptor blockers), β-adrenergic blockers, and most aggressively in unresponsive patients with cardiac transplantation. However, the prognosis of DCM is still poor, so there is a need for new therapeutic approaches. Article p 1691Approximately 20-50% of cases of idiopathic DCM may have a genetic basis. Screening first-degree relatives of a proband with idiopathic DCM by echocardiography and electrocardiography reveals that 20-48% of probands have affected relatives, consistent with a diagnosis of familial DCM. Numerous large kindreds with familial DCM have provided the foundation for establishing genetic causation, and mutations in multiple genes have been shown to cause as shown in the website of GENE Reviews. 3 Current estimates indicate that the 20-plus known familial DCMcausing genes account for only a minority of cases of this disease. To understand its pathology, and with the ultimate hope of finding a treatment, researchers have generated genetic rodent models of heart failure. 4,5 On page 1691 of this issue of the Journal, Ho et al 6 describe their attempt to established a transgenic model of idiopathic DCM in zebrafish using microinjection of tissue-specific and inducible RNA interference with cardiac troponin C (TnC).Myocardial contraction results from interaction of the myosin with the actin in the myocardial thin filament. The thin filament is comprised of actin, tropomyosin and troponin. Troponin is a complex of 3 subunits, troponin T (TnT), troponin I (TnI), and TnC, each of which has distinct roles in the thin filament. Elevated [Ca 2+ ] leads to a conformational change in TnC, increasing its affinity for TnI, while the affinity of TnI for actin decreases. The conformational change in the troponin subunits allows tropomyosin to move out of the way of the actin active sites, so that myosin can attach and produce movement. Thus, TnC plays an important role in the cardiac excitation-contraction coupling process. Interfering with TnC can produce contractile dysfunction. In contrast, the calcium sensitivity of TnC can be enhanced with pimobendan or levosimendan. 7 These calcium sensitizers are attractive therapeutic agents for improving contractile dysfunction in congestive heart failure without increasing oxygen demand or producing Ca 2+ overload. 8 The zebrafish has a highly conserved cardiac TnC amino acid sequence compared with mammals, with a 90% homology in the 4 calcium-binding domains. Since the generation of the first transgenic zebrafish in 1988 by microinjecting naked DNA, 9 numerous transgenic zebrafish models have been developed. Although other app...

show abstract

Novel Rat Model of Ischemic Cardiomyopathy Induced by Repetitive Myocardial Ischemia/Reperfusion Injury While Conscious

Cited by 11 publications

References 36 publications

Conditional Antisense-Knockdown of Zebrafish Cardiac Troponin C as a New Animal Model for Dilated Cardiomyopathy

Conditional Antisense-Knockdown of Zebrafish Cardiac Troponin C as a New Animal Model for Dilated Cardiomyopathy

Development of Cardiac Dysfunction Induced by Repetitive Transient Myocardial Ischaemia Is Inhibited by Edaravone in Conscious Rats

Zebrafish With Antisense-Knockdown of Cardiac Troponin C as a Model of Hereditary Dilated Cardiomyopathy

Contact Info

Product

Resources

About