The effect of fiber length and grammage on the electrophotographic digital printing process was investigated in this study. While integral optical density values on handsheets have shown no significant change, unprinted handsheets have shown an increasing change as short fiber content has increased. Because of structural changes in the paper surface, the measured results for print chroma, brightness, contrast, and delta gloss differed between paper groups. This research confirmed that these changes have nothing to do with grammage or fiber length content. Regardless of the fiber length ratios, significant increases in tone values of ink, were accompanied by a decrease in the grammage of the handsheets among the grammage groups provided. The specimen grammage affected the universal color diagrams, but not the fiber length. The discrepancies in all of these printing outcomes were thought to be caused by the random distribution of fibers and the distances between them on the paper's surface.This article is protected by copyright. All rights reserved.