Novel regimens of phytopolyphenols with cisplatin or memantine and ZnSO4 for synergistic inhibition of growth and gingipains of the cultured Porphyromonas gingivalis

Novel regimens of phytopolyphenols and celecoxib enhancing efficacy and selectivity of anticancer effects of chemotherapeutic agents on cultured cancer cells

“…and metal ions (M; ZnSO 4 ), so called PTM regimens, which produced synergistic and selective anticancer and antibacterial effects. 12 , 19 They were approved by five patents as innovative and creative regimens. 10 In this study, we showed that PTM regimens containing Cl, Mem or TRZ exhibited synergistic anticancer effects.…”

“…Antiproliferative effects of cultured cells (SG, normal gingival epithelium; OECM-1, oral squamous cell carcinoma; A549, lung cancer; DLD-1, colon cancer) and pathogens (P.g., Porphyromonas gingivalis ; UA159, Streptococcus mutans ; P. a., Pseudomonas aeruginosa ; S. a., Staphylococcus aureus ) were assayed by MTT test and optical density (OD600) respectively. 12 IC 50 [concentration (μg/ml, Zn concentation is μM) for 50% inhibition] was calculated from the concentration–inhibition curve. Efflux pump (Na + -K + - ATPase) activity was assayed by colorimetric method as described previously.…”