Novel Retro-Inverso Peptide Antibiotic Efficiently Released by a Responsive Hydrogel-Based System

Cesaro, Angela; Gaglione, Rosa; Chino, Marco; Luca, Maria De; Girolamo, Rocco Di; Lombardi, Angela; Filosa, Rosanna; Arciello, Angela

doi:10.3390/biomedicines10061301

Cited by 8 publications

(4 citation statements)

References 47 publications

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“…We conclude that the antimicrobial manifestation of peptide preparations against bacteria and the effect against Streptococcus mutans are encouraging results. Evidence in caries development places emphasis on the role of S. mutans ; it possesses characteristics linked to dental caries and can attach to other oral bacteria as well as the acquired enamel pellicle (acidogenicity, aciduricity, and the ability to build exopolysaccharides from sucrose) [ 44 , 45 , 46 ].…”

Section: Discussionmentioning

confidence: 99%

Developing Bioactive Hydrogels with Peptides for Dental Application

Muntean,

Sarosi,

Petean

et al. 2024

Biomedicines

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Dental caries is an avoidable and complex condition impacting billions of individuals worldwide, posing a specific concern among younger generations, despite the progress of oral hygiene products. This deterioration occurs due to the acid demineralization of tooth enamel, leading to the loss of minerals from the enamel subsurface. The remineralisation of early enamel carious lesions could prevent the cavitation of teeth. The enamel protein amelogenin constitutes 90% of the total enamel matrix protein and plays a key role in the bio mineralisation process. The aim of this study is to investigate the self-assembly microstructure and reticulation behaviour of a newly developed bioactive hydrogel with leucine-rich amelogenin peptide (LRAP) intended for enamel remineralisation. SEM, AFM, UV-VIS, and FTIR analyses emphasize the ability of peptides to promote cell adhesion and the treatment of early carious lesions. In conclusion, short-chain peptides can be used in hydrogels for individual or professional use.

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Section: Discussionmentioning

confidence: 99%

Developing Bioactive Hydrogels with Peptides for Dental Application

Muntean,

Sarosi,

Petean

et al. 2024

Biomedicines

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“…Hence, ApoB-derived peptides appear promising candidates to be employed as novel food biopreservatives able to counteract bacterial infections without inducing resistance development. Based on this, future experiments will be performed by incorporating peptides in edible films used to wrap food samples [24,63] and to prevent their contamination.…”

Section: Future Perspectivesmentioning

confidence: 99%

Host defense peptides identified in human apolipoprotein B as natural food bio‐preservatives: Evaluation of their biosafety and digestibility

Dell’Olmo

Pane²,

Schibeci

et al. 2023

Peptide Science

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The employment of chemical agents in the food industry is raising several concerns by consumers and is leading to an increasing interest in natural food preservatives. Among alternatives, host defense peptides (HDPs) have attracted great interest for their ability to preserve food samples from contamination without altering their quality, taste, and organoleptic properties. Recently, we evaluated the applicability of ApoB‐derived peptides as novel food bio‐preservatives and demonstrated their ability to prevent chicken meat sample contamination when immobilized on chitosan films. To perform a further step towards the applicability of these peptides in the food field, here we evaluated peptides biosafety and digestibility. To do this, we used a multidisciplinary approach including the evaluation of the peptides' toxicity and antimicrobial activity, the analysis of resistance phenotype development, an in silico prediction of the peptides' susceptibility to proteases and the evaluation of the peptides' stability in simulated gastric and intestinal fluids. ApoB‐derived peptides were found to be nontoxic when tested on human gastric carcinoma cells SNU‐1 and on human colon‐rectal adenocarcinoma cells HT‐29, and not to induce resistance phenotype in Salmonella strains. Bioinformatic analyses showed that the peptides are susceptible to several proteases, as also confirmed by experiments in simulated gastric and intestinal fluids. Altogether these findings open interesting perspectives to the future applicability of ApoB‐derived peptides as novel food biopreservatives.

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“…Encrypted peptides (EPs) were discovered as a result of such efforts. These molecules have now been identified across the tree of life, including in the human proteome 8,10,11 . Many EPs have been found to have potent antimicrobial properties 13 .…”

Section: Introductionmentioning

confidence: 99%

“…; https://doi.org/10.1101/2024.03. 25.586636 doi: bioRxiv preprint molecules have now been identified across the tree of life, including in the human proteome 8,10,11 . Many EPs have been found to have potent antimicrobial properties 13 .…”

Section: Introductionmentioning

confidence: 99%

Peptides from non-immune proteins target infections through antimicrobial and immunomodulatory properties

Torres,

Cesaro,

de la Fuente-Nunez

2024

Preprint

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SummaryEncrypted peptides have been recently described as a new class of antimicrobial molecules. They have been proposed to play a role in host immunity and as alternatives to conventional antibiotics. Intriguingly, many of these peptides are found embedded in proteins unrelated to the immune system, suggesting that immunological responses may extend beyond traditional host immunity proteins. To test this idea, here we synthesized and tested representative peptides derived from non-immune proteins for their ability to exert antimicrobial and immunomodulatory properties. Our experiments revealed that most of the tested peptides from non-immune proteins, derived from structural proteins as well as proteins from the nervous and visual systems, displayed potentin vitroantimicrobial activity. These molecules killed bacterial pathogens by targeting their membrane, and those originating from the same region of the body exhibited synergistic effects when combined. Beyond their antimicrobial properties, nearly 90% of the peptides tested exhibited immunomodulatory effects, modulating inflammatory mediators such as IL-6, TNF-α, and MCP-1. Moreover, eight of the peptides identified, collagenin 3 and 4, zipperin-1 and 2, and immunosin-2, 3, 12, and 13, displayed anti-infective efficacy in two different preclinical mouse models, reducing bacterial infections by up to four orders of magnitude. Altogether, our results support the hypothesis that peptides from non-immune proteins may play a role in host immunity. These results potentially expand our notion of the immune system to include previously unrecognized proteins and peptides that may be activated upon infection to confer protection to the host.

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Novel Retro-Inverso Peptide Antibiotic Efficiently Released by a Responsive Hydrogel-Based System

Cited by 8 publications

References 47 publications

Developing Bioactive Hydrogels with Peptides for Dental Application

Developing Bioactive Hydrogels with Peptides for Dental Application

Host defense peptides identified in human apolipoprotein B as natural food bio‐preservatives: Evaluation of their biosafety and digestibility

Peptides from non-immune proteins target infections through antimicrobial and immunomodulatory properties

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