Novel RNA-binding properties of the MTG chromatin regulatory proteins

Abstract

Background: The myeloid translocation gene (MTG) proteins are non-DNA-binding transcriptional regulators capable of interacting with chromatin modifying proteins. As a consequence of leukemia-associated chromosomal translocations, two of the MTG proteins, MTG8 and MTG16, are fused to the DNA-binding domain of AML1, a transcriptional activator crucial for hematopoiesis. The AML1-MTG fusion proteins, as the wild type MTGs, display four conserved homology regions (NHR1-4) related to the Drosophila nervy protein. Structural protein analyses led us to test the hypothesis that specific MTG domains may mediate RNA binding.

Expression profiling of ETO2‐regulated miRNAs in erythroid cells: Possible influence on miRNA abundance

“…This previous study also indicated that HDAC1 enhances miRNA processing via deacetylation of Drosha/DGCR8 [27]. Thus, based on the observations that ETO2 directly associates with HDAC1 [10] and exerts RNA binding properties [23], we speculate that ETO2 may promote processing of a subset of miRNAs, leading to transcriptional/translational repression. However, we cannot exclude the possibility that ETO2 directly represses other factors, which might lead to the increased pre‐miRNA transcripts, which warrants further analyses.…”

ETO family protein Mtgr1 mediates Prdm14 functions in stem cell maintenance and primordial germ cell formation

“…Prdm14 is a sequence-dependent DNA-binding protein that binds many genomic loci in mESCs, corresponding primarily to distal regulatory elements, whereas ETO proteins do not contain domains implicated in direct DNA sequence recognition ( Rossetti et al, 2004 , 2008 ). To examine whether Mtgr1 is brought to genomic targets occupied by Prdm14, we performed Mtgr1 ChIP coupled with high-throughput DNA sequencing (ChIP-seq) from wt mESCs, FH-Prdm14 overexpressing mESCs, and as a control for antibody specificity, Mtgr1 −/− mESCs (generation of which is described in more detail later), cultured for 5 days under serum+leukemia inhibitory factor (LIF) conditions.…”

Runx1t1 promotes the neuronal differentiation in rat hippocampus

“…This phenomenon was mainly characterized by its four nervy homology regions (NHRs). These NHRs define the domains of Runx1t1 that mediate interactions with other proteins, such as the N-CoR/mSin3A/ HDACs, to form a co-repressor complex for transcription repression [17,30,31]. During early adipogenesis, Runx1t1 may act as an inhibitor of C/EBPβ contributing to its characteristically delayed activation, thereby block transcription of its downstream genes (such as PPARγ), inhibiting preadipocyte differentiation [32].…”