Novel role of RGS2 in regulation of antioxidant homeostasis in neuronal cells

Salim, Samina; Asghar, Mohammad; Taneja, Manish; Hovatta, Iiris; Wu, Yuh Lin; Saha, Kaustuv; Sarraj, Nada; Hite, Brian

doi:10.1016/j.febslet.2011.04.023

Cited by 19 publications

(14 citation statements)

References 25 publications

(40 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is clear that high anxiety (Salim et al 2010a; Salim et al 2010b) corresponds to low GSR1, GLO1 and BDNF while no anxiety (Salim et al 2010a) with high levels of these proteins in these models. In agreement with these findings, our recent publication using a neuronal cell culture model of acute oxidative stress, suggests that GLO1 and GSR1 are transcriptionally up-regulated via Regulator of G-protein Signaling protein (RGS)-2 dependent mechanisms (Salim et al 2011). Sub-chronic oxidative stress in this cell line reduced RGS2, GLO1 and GSR1 mRNA and proteins (unpublished observations).…”

Section: Discussionsupporting

confidence: 67%

Potential contribution of oxidative stress and inflammation to anxiety and hypertension

et al. 2011

Self Cite

View full text Add to dashboard Cite

Previously, we have published that pharmacological induction of oxidative stress causes anxiety-like behavior in rats and also is associated with hypertension in these animals. Here, we report that sub-chronic induction of oxidative stress via pharmacological induction leads to i) reduction in glyoxalase (GLO)-1 and glutathione reductase (GSR)-1 expression; ii) calpain mediated reduction of brain derived neurotrophic factor (BDNF) levels; iii) NFκB mediated upregulation of proinflammatory factors interleukin (IL)-6 and tumor necrosis factor (TNF)-α and elevated angiotensin (AT)-1 receptor levels in hippocampus, amygdala and locus coeruleus regions of the brain. Acute oxidative stress has opposite effects. We speculate that regulation of GLO1, GSR1, BDNF, NFκB and AT-1 receptor may contribute to anxiety-like behavior and hypertension in rats.

show abstract

Section: Discussionsupporting

confidence: 67%

Potential contribution of oxidative stress and inflammation to anxiety and hypertension

et al. 2011

Self Cite

View full text Add to dashboard Cite

show abstract

“…Animal studies on the other hand have been more informative in clarifying role of oxidative stress in anxiety-like behaviors. Several laboratories including ours have reported that pharmacological induction of oxidative stress causes anxiety-like behaviors in rats and prevention of oxidative stress with antioxidant tempol treatment mitigates anxiety-like behaviors [ 3 – 5 ]. These interesting studies prompted us to examine whether behavioral outcome of pharmacological induction of oxidative stress can be mimicked in situations where oxidative stress occurs as a consequence of psychological stress.…”

Section: Introductionmentioning

confidence: 99%

Tempol Treatment Reduces Anxiety-Like Behaviors Induced by Multiple Anxiogenic Drugs in Rats

et al. 2015

Self Cite

View full text Add to dashboard Cite

We have published that pharmacological induction of oxidative stress (OS) causes anxiety-like behavior in rats. Using animal models, we also have established that psychological stress induces OS and leads to anxiety-like behaviors. All evidence points towards the causal role of OS in anxiety-like behaviors. To fully ascertain the role of OS in anxiety-like behaviors, it is reasonable to test whether the pro-anxiety effects of anxiogenic drugs caffeine or N-methyl-beta-carboline-3-carboxamide (FG-7142) can be mitigated using agents that minimize OS. In this study, osmotic pumps were either filled with antioxidant tempol or saline. The pumps were attached to the catheter leading to the brain cannula and inserted into the subcutaneous pocket in the back pocket of the rat. Continuous i.c.v. infusion of saline or tempol in the lateral ventricle of the brain (4.3mmol/day) was maintained for 1 week. Rats were intraperitoneally injected either with saline or an anxiogenic drug one at a time. Two hours later all groups were subjected to behavioral assessments. Anxiety-like behavior tests (open-field, light-dark and elevated plus maze) suggested that tempol prevented anxiogenic drug-induced anxiety-like behavior in rats. Furthermore, anxiogenic drug-induced increase in stress examined via plasma corticosterone and increased oxidative stress levels assessed via plasma 8-isoprostane were prevented with tempol treatment. Protein carbonylation assay also suggested preventive effect of tempol in the prefrontal cortex brain region of rats. Antioxidant protein expression and pro-inflammatory cytokine levels indicate compromised antioxidant defense as well as an imbalance of inflammatory response.

show abstract

“…This protein-coding gene is expressed in several tissues such as CNS, heart, vasculature, kidney, immune system, lungs, bone and ovaries [78,79]. Interestingly, oxidative stress conditions increase RGS2 mRNA levels [79,80]; moreover, RGS2 contributes to the regulation of some enzymes involved in antioxidant defense, namely glyoxalase-1 and glutathione reductase-1 [81]. Finally, RGS2 has been shown to be involved in the pathophysiology of gastrointestinal inflammation and visceral pain, mostly regulating T-cell immunity [79,82,83].…”

Section: Discussionmentioning

confidence: 99%

Nutrigenomic Effects of Long-Term Grape Pomace Supplementation in Dairy Cows

Pauletto

Elgendy

Ianni

et al. 2020

Animals

View full text Add to dashboard Cite

The increasing demand for more animal products put pressure on improving livestock production efficiency and sustainability. In this context, advanced animal nutrition studies appear indispensable. Here, the effect of grape pomace (GP), the polyphenol-rich agricultural by-product, was evaluated on Holstein-Friesian cows’ whole-blood transcriptome, milk production and composition. Two experimental groups were set up. The first one received a basal diet and served as a control, while the second one received a 7.5% GP-supplemented diet for a total of 60 days. Milk production and composition were not different between the group; however, the transcriptome analysis revealed a total of 40 genes significantly affected by GP supplementation. Among the most interesting down-regulated genes, we found the DnaJ heat-shock protein family member A1 (DNAJA1), the mitochondrial fission factor (MFF), and the impact RWD domain protein (IMPACT) genes. The gene set enrichment analysis evidenced the positive enrichment of ‘interferon alpha (IFN-α) and IFN-γ response’, ‘IL6-JAK-STAT3 signaling’ and ‘complement’ genes. Moreover, the functional analysis denoted positive enrichment of the ‘response to protozoan’ and ‘negative regulation of viral genome replication’ biological processes. Our data provide an overall view of the blood transcriptomic signature after a 60-day GP supplementation in dairy cows which mainly reflects a GP-induced immunomodulatory effect.

show abstract

Novel role of RGS2 in regulation of antioxidant homeostasis in neuronal cells

Cited by 19 publications

References 25 publications

Potential contribution of oxidative stress and inflammation to anxiety and hypertension

Potential contribution of oxidative stress and inflammation to anxiety and hypertension

Tempol Treatment Reduces Anxiety-Like Behaviors Induced by Multiple Anxiogenic Drugs in Rats

Nutrigenomic Effects of Long-Term Grape Pomace Supplementation in Dairy Cows

Contact Info

Product

Resources

About