Novel round cells in urine sediment and their clinical implications

Shukuya, Kenichi; Ogura, Sayoko; Tokuhara, Yasunori; Okubo, Shigeo; Yatomi, Yutaka; Tozuka, Minoru; Shimosawa, Tatsuo

doi:10.1016/j.cca.2016.04.017

Cited by 8 publications

(4 citation statements)

References 18 publications

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“…When evaluating new medical tests, it is important to first define the unmet clinical need that the test is aiming to address, as well as the test purpose and role in the clinical care pathway [56,57]. Urinalysis is an integral part of clinical practice, providing important information on the functioning of renal/urogenital and other organ systems, and is a vital part of the diagnostic test menu in clinical laboratories [[1], [2], [3], [4], [5], [6], [7], [8], [9], [10], [11], [12], [13]]. Urinalysis is generally used as a screening test; however, manual microscopy of urine sediment requires a high level of expertise, and is time and labor intensive to perform [25].…”

Section: Discussionmentioning

confidence: 99%

“…Urinalysis is a very common technique used in clinical practice, providing crucial information on the functioning of the kidneys and other organ systems, and aiding clinical decision-making in various diseases, such as diabetes, glomerulonephritis, and suspected urinary tract infections (UTIs) [[1], [2], [3], [4], [5], [6], [7], [8], [9], [10], [11], [12], [13]]. Routine urinalysis involves a two-step approach after initial macroscopic examination, consisting of both physicochemical evaluation and microscopic examination of urine sediment to differentiate and quantify clinically relevant urine particles [[14], [15], [16], [17], [18]].…”

Section: Introductionmentioning

confidence: 99%

“…The increased throughput of these instruments over manual methods enables the reliable and rapid screening of urine samples with reduced labor demands on laboratory staff, thereby improving efficiency and cost-effectiveness [36,37]. Additionally, these instruments can improve reliability and precision by eliminating inter-operator variability, and require a low sample volume [[2], [3], [4], [5], [6], [7], [8], [9], [10], [11], [12]]. Two broad techniques have been developed for automated urine sediment analysis: urinary flow cytometry [33,[38], [39], [40]] and image-based microscopy using a built-in camera [[41], [42], [43]].…”

Section: Introductionmentioning

confidence: 99%

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Automated urinalysis combining physicochemical analysis, on-board centrifugation, and digital imaging in one system: A multicenter performance evaluation of the cobas 6500 urine work area

Cobbaert

Arslan

Martín³

et al. 2019

Practical Laboratory Medicine

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BackgroundWe evaluated the analytical performance of the fully automated cobas® 6500 urine work area and its automated components—cobas u 601 and cobas u 701.Design and methodsThe study was conducted at three European centers using un-centrifuged surplus routine urine samples; all measurements were performed within 2 h of sample collection. Precision, sample carry-over, and method comparisons were evaluated per Clinical and Laboratory Standards Institute guidelines. Method comparisons: cobas u 601 versus Urisys 2400 and cobas u 411 urine test strips; and cobas u 701 versus KOVA® visual microscopy and iQ200 analyzer. Operability and functionality were assessed using questionnaires.ResultsPrecision of the entire cobas 6500 system was within predefined acceptance limits and no significant carry-over was observed. Erythrocytes, leukocytes, nitrites, and protein were in good agreement (≥93%) with cobas u 411 reflectometry. High correlation was shown between the cobas u 701 analyzer and KOVA visual microscopy for red blood cells (RBC; slope, 0.89; Pearson’s r, 0.95) and white blood cells (WBC; slope, 0.96; Pearson’s r, 0.96), demonstrating equivalence of test results. The 97.5% percentile reference values on the cobas u 701 analyzer were 5.3 cells/μL (RBC) and 6.2 cells/μL (WBC). The cobas 6500 system showed good sensitivity for small bacteria (>1 μm) and pathological casts, and the user interface, maintenance wizards, and system design were highly rated by operators.ConclusionsThe fully automated workflow, high precision, and high throughput of the cobas 6500 system have the potential to facilitate standardization of urine screening.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Automated urinalysis combining physicochemical analysis, on-board centrifugation, and digital imaging in one system: A multicenter performance evaluation of the cobas 6500 urine work area

Cobbaert

Arslan

Martín³

et al. 2019

Practical Laboratory Medicine

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“…Therefore, this research finding revealed that the RNA recovery from urine increases with the disease progression and could be an insight into novel urinary biomarker identification in CKD. Further studies are required to correlate cellular type and its content and the RNA yield with disease progression (Shukuya et al, 2016).…”

Section: Rt-qpcr Validationmentioning

confidence: 99%

RNA extraction from urine sediment: A cost-effective protocol for gene expression analysis in renal pathology

2022

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Urine is an appropriate choice of specimen to study the biomarkers for metabolic and renal disorders because it is readily available with less harm to the patients. However, RNA extraction from voided urine is challenging due to the presence of RNases and cell scarcity. This study aims to optimize a protocol for RNA extraction from urine samples for gene expression studies in renal pathology. Hundred and two urine samples were collected from both healthy controls (HC) (𝑛 = 15; 54 ± 11 years) and chronic kidney disease (CKD) patients (𝑛 = 87; 56 ± 10 years) and centrifuged at 6,500 ɡ for 20 min at 4 °C to obtain sediment. RNA was extracted from urine sediments using a phenol-based technique. The extracted RNA was quantified and reverse-transcribed into complementary DNA (cDNA). Reverse transcriptase quantitative polymerase chain reactions (RT-qPCR) were carried out using 2 ng of template cDNA to amplify the housekeeping gene, β2-microglobulin (B2M). The total yield of RNA from CKD and HC samples were 718 ± 164 ng and 790 ± 231 ng, respectively, and a statistically significant difference was not observed between the two study groups (p > 0.05). The urinary RNA recovery was significantly increased with CKD progression (p < 0.05). Further, the results show that urine volume, gender, and serum creatinine level significantly influence the RNA yield in only disease groups (p < 0.05). The mean threshold cycle (Ct) values for B2M amplification of CKD and HC were 27.36 ± 3.09 and 20.97 ± 3.90, respectively. This modified phenol-chloroformbased urinary RNA extraction method is less expensive and does not require pre-and post-purification steps. It provides a higher yield of RNA with less inhibition to qPCR and is sufficient for downstream applications than column-based techniques.

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Urine Cytology

Bostwick¹

2020

Urologic Surgical Pathology

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Novel round cells in urine sediment and their clinical implications

Abstract: Round cells are a novel marker that can be used to predict the need for hemodialysis.

Cited by 8 publications

References 18 publications

Automated urinalysis combining physicochemical analysis, on-board centrifugation, and digital imaging in one system: A multicenter performance evaluation of the cobas 6500 urine work area

Automated urinalysis combining physicochemical analysis, on-board centrifugation, and digital imaging in one system: A multicenter performance evaluation of the cobas 6500 urine work area

RNA extraction from urine sediment: A cost-effective protocol for gene expression analysis in renal pathology

Urine Cytology

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