Novel Salmonella Phage, vB_Sen_STGO-35-1, Characterization and Evaluation in Chicken Meat

Rivera, Dácil; Moreno-Switt, Andrea I.; Denes, Thomas G.; Hudson, Lauren K.; Peters, Tracey L.; Samir, Reham; Aziz, Ramy K.; Noben, Jean-Paul; Wagemans, Jeroen; Dueñas, Fernando

doi:10.3390/microorganisms10030606

Cited by 18 publications

(17 citation statements)

References 96 publications

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“…To determine SNPs in the lytic phage STGO-35-1, we sequenced phage isolated from day 1 and 21. A comprehensive report of genomic characteristics of this phage was recently reported ( Rivera et al., 2022 ) and a short time exposure showed SNPs in receptor binding proteins. Further, in this study, we also identified two SNPs in sequenced phage from day 21, and these two SNPs were found in genes annotated as tails spike proteins, which are involved in recognizing the membrane receptors in the bacteria.…”

Section: Resultsmentioning

confidence: 94%

“…(2018 ). A complete description of the used phage, annotation, and growth conditions is in Rivera et al. (2022 ).…”

Section: Methodsmentioning

confidence: 99%

“…Sequenced phage corresponded to i) reference phage represented by STGO-35-1 before the coevolution, ii) coevolved phage from day 1 from replicate 1, and iii) coevolved phage at day 21 from replicate 1. Phenol/chloroform extraction of DNA and precipitation with ethanol were performed for phage STGO-35-1, as previously described ( Rivera et al, 2022 ). DNA concentration was determined by OD measurement with the Maestro Nano Pro-Spectrophotomether (Maestrogen Inc,.…”

Section: Methodsmentioning

confidence: 99%

“…One scaffold of 47,072 bp with 21,487x of coverage was assembled. Comprehensive genome characteristics are described in Rivera et al. (2022) .…”

Section: Methodsmentioning

confidence: 99%

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Long-Term Interactions of Salmonella Enteritidis With a Lytic Phage for 21 Days in High Nutrients Media

Barrón-Montenegro

Rivera²,

Serrano³

et al. 2022

Front. Cell. Infect. Microbiol.

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Salmonella spp. is a relevant foodborne pathogen with worldwide distribution. To mitigate Salmonella infections, bacteriophages represent an alternative to antimicrobials and chemicals in food animals and food in general. Bacteriophages (phages) are viruses that infect bacteria, which interact constantly with their host. Importantly, the study of these interactions is crucial for the use of phages as a mitigation strategy. In this study, experimental coevolution of Salmonella Enteritidis (S. Enteritidis) and a lytic phage was conducted in tryptic soy broth for 21 days. Transfer to fresh media was conducted daily and every 24 hours, 2 mL of the sample was collected to quantify Salmonella OD600 and phage titter. Additionally, time-shift experiments were conducted on 20 colonies selected on days 1, 12, and 21 to evaluate the evolution of resistance to past (day 1), present (day 12), and future (day 21) phage populations. The behavior of the dynamics was modeled and simulated with mathematical mass-action models. Bacteria and phage from days 1 and 21 were sequenced to determine the emergence of mutations. We found that S. Enteritidis grew for 21 days in the presence and absence of the phage and developed resistance to the phage from day 1. Also, the phage was also able to survive in the media for 21 days, however, the phage titer decreased in approx. 3 logs PFU/mL. The stability of the lytic phage population was consistent with the leaky resistance model. The time-shift experiments showed resistance to phages from day 1 of at least 85% to the past, present, and future phages. Sequencing of S. Enteritidis showed mutations in genes involved in lipopolysaccharide biosynthesis genes rfbP and rfbN at day 21. The phage showed mutations in the tail phage proteins responsible for recognizing the cell surface receptors. These results suggest that interactions between bacteria and phage in a rich resource media generate a rapid resistance to the infective phage but a fraction of the population remains susceptible. Interactions between Salmonella and lytic phages are an important component for the rational use of phages to control this important foodborne pathogen.

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Section: Resultsmentioning

confidence: 94%

“…(2018 ). A complete description of the used phage, annotation, and growth conditions is in Rivera et al. (2022 ).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…One scaffold of 47,072 bp with 21,487x of coverage was assembled. Comprehensive genome characteristics are described in Rivera et al. (2022) .…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Long-Term Interactions of Salmonella Enteritidis With a Lytic Phage for 21 Days in High Nutrients Media

Barrón-Montenegro

Rivera²,

Serrano³

et al. 2022

Front. Cell. Infect. Microbiol.

Self Cite

View full text Add to dashboard Cite

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“…Globally, Salmonella spp. is thought to be responsible for 155,000 fatalities and 93.8 million instances of acute gastroenteritis [82,83]. According to the Centers for Disease Control and Prevention (CDC), Salmonella spp.…”

Section: Salmonellosismentioning

confidence: 99%

Emerging Infectious Food System Related Zoonotic Foodborne Disease – A Threat to Global Food Safety and Nutrition Security

Godwin¹,

Chandrasekaran²,

Smah³

et al. 2023

Foodborne Pathogens - Recent Advances in Control and Detection

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The zoonotic potential of foodborne infections, as well as their capacity to secrete toxins that cause a threat to global food safety and nutrition security and is enough to highlight the gravity of the problem. Feeding the estimated world population of 8.4–8.7 billion, 9.4–10.2 billion, and 11 billion people by 2030, 2050, and 2100, respectively, will require significant increases in crop and animal production, which will increase the agricultural use of antibiotics, water and pesticides and fertilisers, and contact between humans, wild and domestic animals, all of which will have implications for the emergence and spread of infectious agents. Hence, Infectious foodborne zoonotic illnesses are spreading at an unprecedented rate over the world. The evidence that animals are carriers of foodborne diseases is examined in this chapter. The processes through which infectious foodborne zoonosis impacts the global food and nutrition security, as well as how human infectious illnesses may affect food production and distribution are synthesised. The need for agricultural and disease management and policy activities, as well as a review of recent research on novel detection and control techniques in addressing the public health threat posed by foodborne Zoonotic illness, is also addressed.

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Characterization of two virulent Salmonella phages and transient application in egg, meat and lettuce safety

Sun,

Xue,

Cong

et al. 2024

Food Research International

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Novel Salmonella Phage, vB_Sen_STGO-35-1, Characterization and Evaluation in Chicken Meat

Cited by 18 publications

References 96 publications

Long-Term Interactions of Salmonella Enteritidis With a Lytic Phage for 21 Days in High Nutrients Media

Long-Term Interactions of Salmonella Enteritidis With a Lytic Phage for 21 Days in High Nutrients Media

Emerging Infectious Food System Related Zoonotic Foodborne Disease – A Threat to Global Food Safety and Nutrition Security

Characterization of two virulent Salmonella phages and transient application in egg, meat and lettuce safety

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