Novel ‘SiC’ carbosilane dendrimers as carriers for anti-HIV nucleic acids: Studies on complexation and interaction with blood cells

Pedziwiatr-Werbicka, Elzbieta; Fuentes, Elena; Dzmitruk, Volha; Sánchez‐Nieves, Javier; Sudas, Marharyta; Drozd, E. S.; Shakhbazau, Antos; Shcharbin, Dzmitry; Mata, F. Javier de la; Gomez-Ramirez, Rafael; Muñoz‐Fernández, María Ángeles; Bryszewska, Maria

doi:10.1016/j.colsurfb.2013.03.045

Cited by 40 publications

(20 citation statements)

References 41 publications

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“…Other studies of interaction between PAMAM or carbosilane dendrimers with siRNA have demonstrated that dendrimers can slightly change the structure of nucleic acid [4,39], which might be related to the type of dendrimer terminal groups. Decline in ellipticity correlated with increasing dendrimer/siRNA charge ratio, indicating the formation of complexes -a similar effect has been described with other dendrimers [4,37,39]. The Results are mean ± standard deviation (SD), n = 3. siRNAs CD spectra were typical for RNA duplexes A-type, with λ = 210 nm and λ = 260 nm peaks.…”

Section: Discussionsupporting

confidence: 76%

“…Thus, the dendrimers with larger numbers of charges can more easily form complexes with siRNA and at lower concentrations [30,41,44,45]. Similar results had been obtained for PAMAM [4], phosphorus [39] and carbosilane dendrimers [4,37].…”

Section: Discussionmentioning

confidence: 56%

“…Different types of nanoparticles have been studied as potential carriers of oligonucleotides and siRNA [27][28][29][30][31][32][33]. Dendrimers seem to be as excellent non-viral carriers of nucleic acids [34][35][36][37]. PAMAM, phosphorus and carbosilane dendrimers form stable dendriplexes with siRNA [4,28], and have demonstrated that antiapoptotic siRNAs complexed with phosphorus dendrimers and delivered to cells can be internalized, inducting apoptosis.…”

Section: Discussionmentioning

confidence: 99%

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Ruthenium dendrimers as carriers for anticancer siRNA

Michlewska

Йонов

Maroto-Díaz

et al. 2018

Journal of Inorganic Biochemistry

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Dendrimers, which are considered as one of the most promising tools in the field of nanobiotechnology due to their structural organization, showed a great potential in gene therapy, drug delivery, medical imaging and as antimicrobial and antiviral agents. This article is devoted to study interactions between new carbosilane-based metallodendrimers containing ruthenium and anti-cancer small interfering RNA (siRNA). Formation of complexes between anti-cancer siRNAs and Ru-based carbosilane dendrimers was evaluated by transmission electron microscopy, circular dichroism and fluorescence. The zeta-potential and the size of dendriplexes were determined by dynamic light scattering. The internalization of dendriplexes were estimated using HL-60 cells. Results show that ruthenium dendrimers associated with anticancer siRNA have the ability to deliver siRNA as non-viral vectors into the cancer cells. Moreover, dendrimers can protect siRNA against nuclease degradation. Nevertheless, further research need to be performed to examine the therapeutic potential of ruthenium dendrimers as well as dendrimers complexed with siRNA and anticancer drugs towards cancer cells.

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Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 99%

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Ruthenium dendrimers as carriers for anticancer siRNA

Michlewska

Йонов

Maroto-Díaz

et al. 2018

Journal of Inorganic Biochemistry

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“…Carbosilane dendrimers usually possess a C-Si backbone and their bifunctional methylsilane fragments act as the bifurcation/branching points of monomeric unit branches. Several techniques for synthesizing carbosilane dendrimers with a compact tetrafunctional cores (e.g., tetraallylsilane, tetravinylsilane) [ 45 , 46 ] and a more voluminous trifunctional nucleus (trioxybenzene) [ 47 ] have been described. Since the core is relatively small, dendrimers of this class have a high density of functional groups at the periphery.…”

Section: Dendrimersmentioning

confidence: 99%

Dendrimers Show Promise for siRNA and microRNA Therapeutics

et al. 2018

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The lack of an appropriate intracellular delivery system for therapeutic nucleic acids (TNAs) is a major problem in molecular biology, biotechnology, and medicine. A relatively new class of highly symmetrical hyperbranched polymers, called dendrimers, shows promise for transporting small TNAs into both cells and target tissues. Dendrimers have intrinsic advantages for this purpose: their physico-chemical and biological properties can be controlled during synthesis, and they are able to transport large numbers of TNA molecules that can specifically suppress the expression of single or multiple targeted genes. Numerous chemical modifications of dendrimers extend the biocompatibility of synthetic materials and allow targeted vectors to be designed for particular therapeutic purposes. This review summarizes the latest experimental data and trends in the medical application of various types of dendrimers and dendrimer-based nanoconstructions as delivery systems for short small interfering RNAs (siRNAs) and microRNAs at the cell and organism levels. It provides an overview of the structural features of dendrimers, indicating their advantages over other types of TNA transporters.

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“…We have previously reported the properties of phosphorus dendrimers as gene and siRNA transfection reagents in stem cells and other cell types . Dendrimers also emerged as potent antibacterial agents, regulators of natural killer cells, and in numerous other applications …”

Section: Introductionmentioning

confidence: 99%

Fluorescent Phosphorus Dendrimer as a Spectral Nanosensor for Macrophage Polarization and Fate Tracking in Spinal Cord Injury

Shakhbazau

Mishra

Chu

et al. 2015

Macromolecular Bioscience

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Dendrimers and dendriplexes, highly branched synthetic macromolecules, have gained popularity as new tools for a variety of nanomedicine strategies due to their unique structure and properties. We show that fluorescent phosphorus dendrimers are well retained by bone marrow-derived macrophages and exhibit robust spectral shift in its emission in response to polarization conditions. Fluorescence properties of this marker can also assist in identifying macrophage presence and phenotype status at different time points after spinal cord injury. Potential use of a single dendrimer compound as a drug/siRNA carrier and phenotype-specific cell tracer offers new avenues for enhanced cell therapies combined with monitoring of cell fate and function in spinal cord injury.

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Novel ‘SiC’ carbosilane dendrimers as carriers for anti-HIV nucleic acids: Studies on complexation and interaction with blood cells

Cited by 40 publications

References 41 publications

Ruthenium dendrimers as carriers for anticancer siRNA

Ruthenium dendrimers as carriers for anticancer siRNA

Dendrimers Show Promise for siRNA and microRNA Therapeutics

Fluorescent Phosphorus Dendrimer as a Spectral Nanosensor for Macrophage Polarization and Fate Tracking in Spinal Cord Injury

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