Novel signal amplification strategy for ultrasensitive sandwich-type electrochemical immunosensor employing Pd–Fe3O4-GS as the matrix and SiO2 as the label

Wang, Yulan; Ma, Hongmin; Wang, Xiaodong; Pang, Xuehui; Wu, Dan; Du, Bin; Wei, Qin

doi:10.1016/j.bios.2015.06.033

Cited by 51 publications

(11 citation statements)

References 47 publications

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“…The main effect of these experimental conditions is the value of pH, since the solution of acidic or alkaline may damage the antigen-antibody linkage and inactivate the activity of biomolecules 29 30 . In other words, the antigens and antibodies will keep their bioactivity in the approximate neutral conditions of pH 31 . The Fig.…”

Section: Resultsmentioning

confidence: 99%

Ultrasensitive sandwich-type electrochemical immunosensor based on trimetallic nanocomposite signal amplification strategy for the ultrasensitive detection of CEA

Tian

Liu

et al. 2016

Sci Rep

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A novel and ultrasensitive sandwich-type electrochemical immunosensor was designed for the quantitative detection of carcino-embryonic antigen (CEA). This immunosensor was developed by using the trimetallic NiAuPt nanoparticles on graphene nanosheets (NGs) nanosheets (NiAuPt-NGs) as excellent labels and β-cyclodextrin functionalized reduced graphene oxide nanosheets (CD-NGs) as the platform. The CD-NGs with high specific surface area good biocompatibility and the ideal dispersibility was used to capture the primary antibodies (Ab1) efficiently. The trimetallic NiAuPt-NGs nanocomposites were used as the labels for signal amplification, showing better electrocatalytic activity towards the reduction of hydrogen peroxide (H2O2), which is much better than that the monometallic Pt-NGs, bimetallic NiPt-NGs and AuPt-NGs due to the synergetic effect presented in NiAuPt-NGs. The NiAuPt-NGs nanocomposites consist of tightly coupled nanostructures of Au, Ni and Pt, which have neither an alloy nor a core-shell structure. Under the optimal conditions, a linear range from 0.001–100 ng/mL and a low detection limit of 0.27 pg/mL were obtained for CEA. The proposed electrochemical sandwich-type immunosensor may have a promising application in bioassay and it enriches the electrochemical immunoassays.

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Section: Resultsmentioning

confidence: 99%

Ultrasensitive sandwich-type electrochemical immunosensor based on trimetallic nanocomposite signal amplification strategy for the ultrasensitive detection of CEA

Tian

Liu

et al. 2016

Sci Rep

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“…Due to the unique size-dependent magnetic properties, IONs are used in a wide variety of fields, including nanocatalysis, all-optical switching devices, microwave absorption, environmental remediation, chemical and biological sensors, energy generation and storage, biomedicine and biotechnologies, etc. [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 ]. Among various IONs, magnetite (Fe 3 O 4 ) nanoparticles are one of the most promising, since they find application in such areas as high-density magnetic storage media, capacitor electrodes, development of sensors, ferrofluid, for absorption heavy metals [ 5 , 13 , 14 , 15 , 16 , 17 , 18 , 19 ], have wide application in biotechnologies and biomedicine, for example, magnetic resonance imaging, hyperthermia, targeted delivery of drugs, detect and treat cancer [ 20 , 21 , 22 , 23 , 24 , 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

Magnetic and Magneto-Optical Oroperties of Iron Oxides Nanoparticles Synthesized under Atmospheric Pressure

et al. 2020

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Magnetite nanoparticles were synthesized by a simple thermal decomposition process, involving only iron (III) nitrate nonahydrate as a precursor, and hexadecylamine as a solvent and stabilizer at reaction temperatures varied from 200 to 380 °C. The results of the structural analysis showed that the average crystallite size depends on the reaction temperature and increases from 4.8 to 13.3 nm. The behavior of the coercivity indicates that all synthesized samples are single domain; herewith, it was found that the critical size corresponding to the transition to the superparamagnetic state at room temperature is about 9 nm. The effect of the reaction temperature on changes in the saturation magnetization was studied. It was found that the size effect in the MCD spectra is observed for the IVCT transition and one ISCT transition, and the influence of the reaction temperature on the change in the MCD spectra was discussed.

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“…Simultaneously, the highly acidic or alkaline surroundings would influence the activity of the antigens, antibodies and damage the immobilized protein 48 49 . By contrast, the antigens and antibodies could keep their bioactivity in this approximate neutral conditions of pH 50 .…”

Section: Resultsmentioning

confidence: 97%

An ultrasensitive label-free electrochemical immunosensor based on signal amplification strategy of multifunctional magnetic graphene loaded with cadmium ions

Dong

et al. 2016

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Herein, a novel and ultrasensitive label-free electrochemical immunosensor was proposed for quantitative detection of human Immunoglobulin G (IgG). The amino functionalized magnetic graphenes nanocomposites (NH2-GS-Fe3O4) were prepared to bond gold and silver core-shell nanoparticles (Au@Ag NPs) by constructing stable Au-N and Ag-N bond between Au@Ag NPs and -NH2. Subsequently, the Au@Ag/GS-Fe3O4 was applied to absorb cadmium ion (Cd2+) due to the large surface area, high conductivity and exceptional adsorption capability. The functional nanocomposites of gold and silver core-shell magnetic graphene loaded with cadmium ion (Au@Ag/GS-Fe3O4/Cd2+) can not only increase the electrocatalytic activity towards hydrogen peroxide (H2O2) but also improve the effective immobilization of antibodies because of synergistic effect presented in Au@Ag/GS-Fe3O4/Cd2+, which greatly extended the scope of detection. Under the optimal conditions, the proposed immunosensor was used for the detection of IgG with good linear relation in the range from 5 fg/mL to 50 ng/mL with a low detection limit of 2 fg/mL (S/N = 3). Furthermore, the proposed immunosensor showed high sensitivity, special selectivity and long-term stability, which had promising application in bioassay analysis.

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Novel signal amplification strategy for ultrasensitive sandwich-type electrochemical immunosensor employing Pd–Fe3O4-GS as the matrix and SiO2 as the label

Cited by 51 publications

References 47 publications

Ultrasensitive sandwich-type electrochemical immunosensor based on trimetallic nanocomposite signal amplification strategy for the ultrasensitive detection of CEA

Ultrasensitive sandwich-type electrochemical immunosensor based on trimetallic nanocomposite signal amplification strategy for the ultrasensitive detection of CEA

Magnetic and Magneto-Optical Oroperties of Iron Oxides Nanoparticles Synthesized under Atmospheric Pressure

An ultrasensitive label-free electrochemical immunosensor based on signal amplification strategy of multifunctional magnetic graphene loaded with cadmium ions

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