Novel Signaling from the Peripodial Membrane Is Essential for Eye Disc Patterning in Drosophila

Cho, Kyung-Ok; Chern, Joshua J.; Izaddoost, Shayan A.; Choi, Kwang‐Wook

doi:10.1016/s0092-8674(00)00124-0

Cited by 128 publications

(133 citation statements)

References 62 publications

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“…Consistent with this model, the lack of eye development in mutants for eyegone, a target of Notch signaling that is required for disc growth (33), can be rescued by inhibiting Wg signaling in posterior cells (34). In the third instar eye disc, wg expression is restricted to the anterior lateral margins (6,13,15,29,35), where it continues to repress the expression of Eya, So and Dac in regions destined to form the dorsal head (30). Wg may also promote the head fate by enhancing expression of the anteriorly expressed transcription factor Hth (36) (Fig.…”

Section: Wg Inhibits Eye Specificationmentioning

confidence: 99%

“…It is not clear when dorsal-ventral differences are first established, as transcripts encoding the GATA family transcription factor Pannier (Pnr) are present in the dorsalmost region of the embryonic eye disc (41), but a pnr-GAL4 line does not drive UAS-GFP expression in the dorsal eye disc until early second instar (42). wg-lacZ is expressed in the dorsal peripodial membrane and margin cells beginning in the first instar (6,35,43); at least at later stages, dorsal wg expression is dependent on pnr (41). wg expression in the dorsal peripodial epithelium has been traced to activation by Pnr of an eye-specific enhancer in the wg 3' cis-regulatory region (44).…”

Section: Wg Patterns the Dorsal-ventral Axis In The Eye Discmentioning

confidence: 99%

“…Final eye size must depend on the interplay between Wg and other growth regulators such as Dpp, Notch and its target Upd (6,(46)(47)(48)(54)(55)(56). Expression of Wg or activated Arm throughout the eye disc reduces eye size in addition to blocking differentiation (31,34,49), probably due to its effects on these other factors.…”

Section: Wg Controls Growth Of the Eye Discmentioning

confidence: 99%

“…X-gal staining buffer: For 50ml, mix 1.8ml 0.2M Na 2 HPO4, 0.7ml 0.2M NaH 2 PO4, 1.5ml 5M NaCl, 50µl 1M MgCl 2 ,, 3.05ml 50mM K 3 Fe(CN) 6 , 3.05ml 50mM K 4 Fe (CN) 6 , H 2 O to 50ml. …”

Section: )mentioning

confidence: 99%

“…The columnar epithelium of the disc is partitioned into separate eye and antennal primordia in the second larval instar (5). The squamous peripodial epithelium gives rise to head structures and may also communicate with the columnar epithelium (6,7).…”

Section: Introductionmentioning

confidence: 99%

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Wingless Signaling in Drosophila Eye Development

Legent

Treisman

2008

Methods in Molecular Biology

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The Drosophila eye disc gives rise to both the retina and the surrounding head capsule. The secreted morphogen Wingless (Wg) plays an important role in subdividing the disc between these two tissues; wg is expressed in the anterior lateral margins, regions that will give rise to the head capsule, and high levels of Wg signaling promote this fate. Wg is expressed earlier and more strongly in the dorsal than the ventral margin, and also contributes to specifying dorsal identity. In addition, Wg signaling can cause overgrowth of eye disc cells. Finally, at pupal stages, wg expression surrounds the eye and a gradient of Wg establishes several distinct peripheral cell fates. We describe how to generate clones of cells mutant for genes encoding components of the Wg signaling pathway in the eye disc and examine their effects on photoreceptor differentiation by antibody staining.

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Section: Wg Inhibits Eye Specificationmentioning

confidence: 99%

Section: Wg Patterns the Dorsal-ventral Axis In The Eye Discmentioning

confidence: 99%

Section: Wg Controls Growth Of the Eye Discmentioning

confidence: 99%

“…X-gal staining buffer: For 50ml, mix 1.8ml 0.2M Na 2 HPO4, 0.7ml 0.2M NaH 2 PO4, 1.5ml 5M NaCl, 50µl 1M MgCl 2 ,, 3.05ml 50mM K 3 Fe(CN) 6 , 3.05ml 50mM K 4 Fe (CN) 6 , H 2 O to 50ml. …”

Section: )mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Wingless Signaling in Drosophila Eye Development

Legent

Treisman

2008

Methods in Molecular Biology

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Drosophila Retinal Patterning

Spencer¹,

Johnson²,

Setu³

2020

Encyclopedia of Life Sciences

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The Drosophila eye is an outstanding model system for exploring fundamental mechanisms of growth and development. The adult eye is composed of a perfect hexagonal lattice of ∼750 unit eyes, or ommatidia, each containing precisely 20 well‐characterized cells. The eye develops from the eye/antennal imaginal disc, a flattened epithelial sac. During larval and pupal development, cells in the disc grow and undergo compartmentalisation, cell cycle arrest, differentiation, directed movement, and apoptosis, all utilising gene networks and signalling pathways similar to those in vertebrates. The genetic accessibility of Drosophila , together with the precision of eye development, makes the fly retina an extremely useful system with which to investigate the roles of genes and signalling pathways in development. Key Concepts Adult Drosophila eyes contain ∼750 ommatidia, or unit eyes; each ommatidium is made up of precisely 20 cells, each of which can be identified by its position and protein expression. Like other adult structures in Drosophila , the eye develops from an imaginal disc, a flattened sac of epithelial cells that develops during larval and pupal stages. Drosophila retinal development utilises gene regulatory networks similar to those in human eye development. Photoreceptor differentiation requires passage of the morphogenetic furrow, an invagination in eye disc that moves across the tissue from posterior to anterior during larval development. Photoreceptors differentiate during larval stages using signals emanating from previously differentiated cells, rather than through lineage restrictions. During pupal development, accessory cells differentiate and apoptosis reduces the number of cells in each ommatidium to 20. Cell interactions during pupal development produce the precise hexagonal lattice characteristic of the adult Drosophila eye.

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Drosophila peripodial cells, more than meets the eye?

Gibson

Schubiger

2001

BioEssays

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Drosophila imaginal discs (appendage primordia) have proved invaluable for deciphering cellular and molecular mechanisms of animal development. By combining the accessibility of the discs with the genetic tractability of the fruit fly, researchers have discovered key mechanisms of growth control, pattern formation and long-range signaling. One of the principal experimental attractions of discs is their anatomical simplicity - they have long been considered to be cellular monolayers. During larval stages, however, the growing discs are 2-sided sacs composed of a columnar epithelium on one side and a squamous 'peripodial' epithelium on the other. Recent studies suggest important roles for peripodial epithelia in processes previously assumed to be confined to columnar cell monolayers.

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Novel Signaling from the Peripodial Membrane Is Essential for Eye Disc Patterning in Drosophila

Cited by 128 publications

References 62 publications

Wingless Signaling in Drosophila Eye Development

Wingless Signaling in Drosophila Eye Development

Drosophila Retinal Patterning

Drosophila peripodial cells, more than meets the eye?

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