Novel signaling mechanisms in the ovary during oocyte maturation and ovulation

Conti, Marco; Hsieh, Minnie; Zamah, A.M.; Oh, Jeong Su

doi:10.1016/j.mce.2011.11.002

Cited by 335 publications

(249 citation statements)

References 109 publications

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“…PDE1-4, PDE7-8 and PDE10-11 all catalyse hydrolysis of cAMP, while the remaining three PDE families solely hydrolyse cGMP (Azevedo et al 2014). In the rodent ovary, PDE4D and PDE3 are known to exert important roles in regulation of oocyte maturation and ovulation (Tsafriri et al 1996, Park et al 2003, Conti et al 2012. In addition, the presence of PDE8 has been reported in the bovine and human ovary (Sasseville et al 2009, Petersen et al 2015 but has not been studied in rodents.…”

Section: Introductionmentioning

confidence: 99%

Phosphodiesterases in the rat ovary: effect of cAMP in primordial follicles

2015

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Phosphodiesterases (PDEs) are important regulators of the intracellular cAMP concentration, which is a central second messenger that affects a multitude of intracellular functions. In the ovaries, cAMP exerts diverse functions, including regulation of ovulation and it has been suggested that augmented cAMP levels stimulate primordial follicle growth. The present study examined the gene expression, enzyme activity and immunolocalization of the different cAMP hydrolysing PDEs families in the rat ovary. Further, the effect of PDE4 inhibition on primordial follicle activation in cultured neonatal rat ovaries was also evaluated. We found varied expression of all eight families in the ovary with Pde7b and Pde8a having the highest expression each accounting for more than 20% of the total PDE mRNA. PDE4 accounted for 15-26% of the total PDE activity. Immunoreactive PDE11A was found in the oocytes and PDE2A in the corpora lutea. Incubating neonatal rat ovaries with PDE4 inhibitors did not increase primordial follicle activation or change the expression of the developing follicle markers Gdf9, Amh, Inha, the proliferation marker Mki67 or the primordial follicle marker Tmeff2. In addition, the cAMP analogue 8-bromo-cAMP did not increase AKT1 or FOXO3A phosphorylation associated with follicle activation or increase the expression of Kitlg known to be associated with follicle differentiation but did increase the Tmeff2, Mki67 and Inha expression in a dose-dependent manner. In conclusion, this study shows that both Pde7b and Pde8a are highly expressed in the rodent ovary and that PDE4 inhibition does not cause an increase in primordial follicle activation. Reproduction (2015) 150 11-20

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Section: Introductionmentioning

confidence: 99%

Phosphodiesterases in the rat ovary: effect of cAMP in primordial follicles

2015

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“…Cyclic guanosine monophosphate (cGMP) diffuses from granulosa cells to the oocyte and inhibits cAMP degradation by inhibiting PDE3A [5,6]. In rodents, elevated cAMP concentrations activate protein kinase-A (PKA), followed by complex inhibitory mechanisms involving CDK1 -cyclin B kinase proteins suppressing activation of maturation promoting factor (MPF) and preventing meiosis [7][8][9]. In vivo, the pre-ovulatory surge of LH decreases cGMP concentrations in granulosa cells as well as oocytes [5,6]; the subsequent activation of PDE3A resulted in degradation of cAMP, activation of MPF, and resumption of meiosis (review; Conti et.…”

Section: Introductionmentioning

confidence: 99%

“…al. [7]). The downstream pathways through which cAMP effects meiosis are not fully characterised in larger mammals like cattle [10] or sheep.…”

Section: Introductionmentioning

confidence: 99%

Regulation of sheep oocyte maturation using cAMP modulators

et al. 2013

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“…This arrest, maintained by cyclic adenosine monophosphate (cAMP) levels within the oocyte, is derived from a constitutive stimulating G-protein, the GPR3 [52]. Ledent et al [53] generated Gpr3 knockout mice to assess their phenotype.…”

Section: Wingless-type Mmtv Integration Site Family Member 4 (Wnt4)mentioning

confidence: 99%