Novel silica-forming peptides derived from Ectocarpus siliculosus

Yeo, Ki Baek; Ki, Mi-Ran; Park, Ki Sung; Pack, Seung Pil

doi:10.1016/j.procbio.2017.04.004

Cited by 26 publications

(9 citation statements)

References 19 publications

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“…1 a). The silica-forming Ectp1 peptide has been known to be capable of binding to mineral surface as well as allowing rapid silica deposition from silicic acid [ 20 ]. The sequence of negatively charged hexaglutamate (E6) was fused into the N-terminus of Ectp1 peptide, enabling the physical adsorption onto the surface of β-TCP particles via ionic interactions with the underlying positively charged calcium ions.…”

Section: Resultsmentioning

confidence: 99%

“…Considering that the mechanical property of hybrid composite strongly depends on interfacial bridging between organic polymer and inorganic particles [ 18 ], the incorporation of β-TCP with collagen (Col/β-TCP) could be an attractive approach to accomplish tailored delivery of BMP-2 and essential mechanical stiffness at different stage of bone regeneration. Second, the skeletal architectures of brown algae are composed of hierarchical silica nanostructures containing organic components that rapidly form silica nanoparticles (SiNPs) under physiological conditions [ 19 , 20 ]. This natural biosilica formation for protection and mechanical support has provided a captivating source as a more attractive approach for silica synthesis in biomedical applications over the conventional sol-gel methods due to mild processing in ambient temperature and pH without the presence of hazardous chemicals.…”

Section: Introductionmentioning

confidence: 99%

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Biosilicated collagen/β-tricalcium phosphate composites as a BMP-2-delivering bone‐graft substitute for accelerated craniofacial bone regeneration

Lee

Kim

et al. 2021

Biomater Res

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Background Bioceramic β-tricalcium phosphate (β-TCP) is used as a bone-grafting material and a therapeutic drug carrier for treatment of bone defects in the oral and maxillofacial regions due to the osteoconductivity and biocompatibility. However, the low mechanical strength and limited osteoinductivity of β-TCP agglomerate restrict bone regenerating performance in clinical settings. Methods Herein, a biomimetic composite is proposed as a bone morphogenetic protein-2 (BMP-2)-delivering bone graft substitute to achieve a robust bone grafting and augmented bone regeneration. Results The sequential processes of brown algae-inspired biosilicification and collagen coating on the surface of β-TCP enable the effective incorporation of BMP-2 into the coating layer without losing its bioactivity. The sustained delivery of BMP-2 from the biosilicated collagen and β-TCP composites promoted in vitro osteogenic behaviors of pre-osteoblasts and remarkedly accelerated in vivo bone regeneration within a rat calvarial bone defect. Conclusions Our multicomposite bone substitutes can be practically applied to improve bone tissue growth in bone grafting applications with further expansion to general bone tissue engineering.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biosilicated collagen/β-tricalcium phosphate composites as a BMP-2-delivering bone‐graft substitute for accelerated craniofacial bone regeneration

Lee

Kim

et al. 2021

Biomater Res

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“…The EctP1 peptide (SSRSSSHRRHDHHDHRRGS) was genetically fused to the C-terminus of the bioengineered mussel adhesive protein (MAP) and named sMAP. sMAP was produced in an Escherichia coli expression system and purified following a previously reported method .…”

Section: Methodsmentioning

confidence: 99%

“…It has been reported that EctP1 has a superior silica-forming ability to the R5 peptide, which has been commonly used for biosilica formation. 27…”

Section: ■ Introductionmentioning

confidence: 99%

“…Coccolith, which has a biocalcificated porous architecture with osteoconductive ability, was prepared and complemented by an osteoinductive biosilica coating using the silica-forming peptide EctP1, which was derived from the brown algae Ectocarpus siliculosus. It has been reported that EctP1 has a superior silica-forming ability to the R5 peptide, which has been commonly used for biosilica formation …”

Section: Introductionmentioning

confidence: 99%

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Bone Graft Biomineral Complex Coderived from Marine Biocalcification and Biosilicification

Yun

Jeong

Nam

et al. 2021

ACS Appl. Bio Mater.

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Bone graft materials have been mainly developed based on inorganic materials, including calcium phosphate. However, these graft materials usually act as osteoconductive rather than osteoinductive scaffolds. To improve bone reconstruction, a combination of several materials has been proposed. However, there are still no alternatives that can completely replace the existing animal-derived bone graft materials. In this work, a marine-inspired biomineral complex was suggested as a potential bone graft material. The proposed biosilicified coccolithophorederived coccoliths using bioengineered mussel adhesive proteins show osteopromotive ability through the synergistic effects of osteoconductivity from calcium carbonate and osteoinductivity from silica. Its possibility of use as a bone substitute was determined by evaluating the in vitro osteogenic behaviors of multipotent mesenchymal stem cells and in vivo bone regeneration in a rat calvarial defect model. Therefore, the marine-inspired biomineral complex developed in this study could be successfully used for bone tissue engineering.

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Production of TiO2-deposited Diatoms and Their Applications for Photo-catalytic Degradation of Aqueous Pollutants

Kumari

Min

Kanth

et al. 2020

Biotechnol Bioproc E

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Novel silica-forming peptides derived from Ectocarpus siliculosus

Cited by 26 publications

References 19 publications

Biosilicated collagen/β-tricalcium phosphate composites as a BMP-2-delivering bone‐graft substitute for accelerated craniofacial bone regeneration

Biosilicated collagen/β-tricalcium phosphate composites as a BMP-2-delivering bone‐graft substitute for accelerated craniofacial bone regeneration

Bone Graft Biomineral Complex Coderived from Marine Biocalcification and Biosilicification

Production of TiO2-deposited Diatoms and Their Applications for Photo-catalytic Degradation of Aqueous Pollutants

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