Novel SIMS system with focused massive cluster ion source for mass imaging spectrometry with high lateral resolution

Matsuo, Jiro; Torii, Souta; Yamauchi, Kenta; Wakamoto, Keisuke; Kusakari, Masakazu; Nakagawa, Shunichiro; Fujii, Makiko; Aoki, Takaaki; Seki, Toshio

doi:10.7567/apex.7.056602

Cited by 32 publications

(19 citation statements)

References 29 publications

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“…The GCIB ions are not easily focused, but have been utilized in a dual beam environment for removing residual damage accumulation left by the analyzing beam, resulting in improved molecular depth profiling [5, 10]. A tightly focused Ar 1000 + cluster ion beam has been reported to imaging organic grid with ~4 μm beam diameter [11]. Another popular ion source utilizes C 60 + ions.…”

Section: Introductionmentioning

confidence: 99%

CO₂ Cluster Ion Beam, an Alternative Projectile for Secondary Ion Mass Spectrometry

Tian

Maciążek

Postawa

et al. 2016

J. Am. Soc. Mass Spectrom.

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The emergence of argon - based gas cluster ion beams for SIMS experiments opens new possibilities for molecular depth profiling and 3D chemical imaging. These beams generally leave less surface chemical damage and yield mass spectra with reduced fragmentation when compared to smaller cluster projectiles. For nanoscale bioimaging applications, however, limited sensitivity due to low ionization probability and technical challenges of beam focusing remain problematic. The use of gas cluster ion beams based upon systems other than argon offer an opportunity to resolve these difficulties. Here we report on the prospects of employing CO2 as a simple alternative to argon. Ionization efficiency, chemical damage, sputter rate and beam focus are investigated on model compounds using a series of CO2 and Ar cluster projectiles (cluster size 1000~5000) with the same mass. The results show that the two projectiles are very similar in each of these aspects. Computer simulations comparing the impact of Ar2000 and (CO2)2000 on an organic target also confirm that the CO2 molecules in the cluster projectile remain intact, acting as a single particle of m/z 44. The imaging resolution employing CO2 cluster projectiles is improved by more than a factor of 2. The advantage of CO2 vs Ar is also related to the increased stability which, in addition, facilitates the operation of the GCIB system at lower backing pressure.

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Section: Introductionmentioning

confidence: 99%

CO₂ Cluster Ion Beam, an Alternative Projectile for Secondary Ion Mass Spectrometry

Tian

Maciążek

Postawa

et al. 2016

J. Am. Soc. Mass Spectrom.

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“…25 The Ar-GCIB has also been successfully used for imaging of biological tissue with satisfactory lateral resolution. 26 The advances in this technique have made lateral resolutions of a few micrometers possible for imaging a metal grid 27 as well as biological samples. 28 In addition, high mass species, such as phosphatidylcholine (PC) lipid dimers of approximately m / z 1545, were detected.…”

mentioning

confidence: 99%

Gas Cluster Ion Beam Time-of-Flight Secondary Ion Mass Spectrometry High-Resolution Imaging of Cardiolipin Speciation in the Brain: Identification of Molecular Losses after Traumatic Injury

et al. 2017

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Gas cluster ion beam-secondary ion mass spectrometry (GCIB-SIMS) has shown the full potential of mapping intact lipids in biological systems with better than 10 μm lateral resolution. This study investigated further the capability of GCIB-SIMS in imaging high-mass signals from intact cardiolipin (CL) and gangliosides in normal brain and the effect of a controlled cortical impact model (CCI) of traumatic brain injury (TBI) on their distribution. A combination of enzymatic and chemical treatments was employed to suppress the signals from the most abundant phospholipids (phosphatidylcholine (PC) and phosphatidylethanolamine (PE)) and enhance the signals from the low-abundance CLs and gangliosides to allow their GCIB-SIMS detection at 8 and 16 μm spatial resolution. Brain CLs have not been observed previously using other contemporary imaging mass spectrometry techniques at better than 50 μm spatial resolution. High-resolution images of naive and injured brain tissue facilitated the comparison of CL species across three multicell layers in the CA1, CA3, and DG regions of the hippocampus. GCIB-SIMS also reliably mapped losses of oxidizable polyunsaturated CL species (but not the oxidation-resistant saturated and monounsaturated gangliosides) to regions including the CA1 and CA3 of the hippocampus after CCI. This work extends the detection range for SIMS measurements of intact lipids to above m/z 2000, bridging the mass range gap compared with MALDI. Further advances in high-resolution SIMS of CLs, with the potential for single cell or supra-cellular imaging, will be essential for the understanding of CL’s functional and structural organization in normal and injured brain.

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“…The experiments were carried out in a new custom-built SIMS instrument in the group of Jiro Matsuo at Kyoto University [44,45] which consists of a gas cluster ion source and primary beam line and an orthogonal-acceleration time-of-flight mass spectrometer (TOF-MS) for the detection of sputtered secondary ions. In the ion source, neutral Ar clusters are formed by the supersonic expansion of a high-pressure gas (∼0.35 MPa) through a nozzle (0.1 mm diameter) and are then introduced into the ionizing chamber.…”

Section: Methodsmentioning

confidence: 99%

Molecular cluster emission in sputtering of amino acids by argon gas-cluster ions

Kusakari

Gnaser

Fujii

et al. 2015

International Journal of Mass Spectrometry

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Novel SIMS system with focused massive cluster ion source for mass imaging spectrometry with high lateral resolution

Cited by 32 publications

References 29 publications

CO₂ Cluster Ion Beam, an Alternative Projectile for Secondary Ion Mass Spectrometry

CO₂ Cluster Ion Beam, an Alternative Projectile for Secondary Ion Mass Spectrometry

Gas Cluster Ion Beam Time-of-Flight Secondary Ion Mass Spectrometry High-Resolution Imaging of Cardiolipin Speciation in the Brain: Identification of Molecular Losses after Traumatic Injury

Molecular cluster emission in sputtering of amino acids by argon gas-cluster ions

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Novel SIMS system with focused massive cluster ion source for mass imaging spectrometry with high lateral resolution

Cited by 32 publications

References 29 publications

CO2 Cluster Ion Beam, an Alternative Projectile for Secondary Ion Mass Spectrometry

CO2 Cluster Ion Beam, an Alternative Projectile for Secondary Ion Mass Spectrometry

Gas Cluster Ion Beam Time-of-Flight Secondary Ion Mass Spectrometry High-Resolution Imaging of Cardiolipin Speciation in the Brain: Identification of Molecular Losses after Traumatic Injury

Molecular cluster emission in sputtering of amino acids by argon gas-cluster ions

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CO₂ Cluster Ion Beam, an Alternative Projectile for Secondary Ion Mass Spectrometry

CO₂ Cluster Ion Beam, an Alternative Projectile for Secondary Ion Mass Spectrometry