Novel sources of resistance to fusarium wilt in Luffa species

Bindal, Sumant; Sheu, Z.M.; Kenyon, L.; Taher, Dalia; Rakha, Mohamed

doi:10.3389/fpls.2023.1116006

Cited by 4 publications

(4 citation statements)

References 39 publications

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“…It’s essential to emphasize that luffa samples from Malaysia exhibited the highest ratio of plants resistant to Fusarium wilt, while none of the sample from Laos showed resistance. Beyond the potential influence of our sample collection methods and the specific Fusarium species or isolates used for inoculation, which may impact luffa resistance [ 37 ], another plausible explanation for this difference could be the influence of the plants’ geographic origins. The country where luffa plants originated might significantly affect their ability to resist Fusarium wilt due to geographical isolation.…”

Section: Discussionmentioning

confidence: 99%

Analyzing genetic diversity in luffa and developing a Fusarium wilt-susceptible linked SNP marker through a single plant genome-wide association (sp-GWAS) study

Li,

Liu,

Jiang

et al. 2024

BMC Plant Biol

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Background Luffa (Luffa spp.) is an economically important crop of the Cucurbitaceae family, commonly known as sponge gourd or vegetable gourd. It is an annual cross-pollinated crop primarily found in the subtropical and tropical regions of Asia, Australia, Africa, and the Americas. Luffa serves not only as a vegetable but also exhibits medicinal properties, including anti-inflammatory, antidiabetic, and anticancer effects. Moreover, the fiber derived from luffa finds extensive applications in various fields such as biotechnology and construction. However, luffa Fusarium wilt poses a severe threat to its production, and existing control methods have proven ineffective in terms of cost-effectiveness and environmental considerations. Therefore, there is an urgent need to develop luffa varieties resistant to Fusarium wilt. Single-plant GWAS (sp-GWAS) has been demonstrated as a promising tool for the rapid and efficient identification of quantitative trait loci (QTLs) associated with target traits, as well as closely linked molecular markers. Results In this study, a collection of 97 individuals from 73 luffa accessions including two major luffa species underwent single-plant GWAS to investigate luffa Fusarium wilt resistance. Utilizing the double digest restriction site associated DNA (ddRAD) method, a total of 8,919 high-quality single nucleotide polymorphisms (SNPs) were identified. The analysis revealed the potential for Fusarium wilt resistance in accessions from both luffa species. There are 6 QTLs identified from 3 traits, including the area under the disease progress curve (AUDPC), a putative disease-resistant QTL, was identified on the second chromosome of luffa. Within the region of linkage disequilibrium, a candidate gene homologous to LOC111009722, which encodes peroxidase 40 and is associated with disease resistance in Cucumis melo, was identified. Furthermore, to validate the applicability of the marker associated with resistance from sp-GWAS, an additional set of 21 individual luffa plants were tested, exhibiting 93.75% accuracy in detecting susceptible of luffa species L. aegyptiaca Mill. Conclusion In summary, these findings give a hint of genome position that may contribute to luffa wild resistance to Fusarium and can be utilized in the future luffa wilt resistant breeding programs aimed at developing wilt-resistant varieties by using the susceptible-linked SNP marker.

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Section: Discussionmentioning

confidence: 99%

Analyzing genetic diversity in luffa and developing a Fusarium wilt-susceptible linked SNP marker through a single plant genome-wide association (sp-GWAS) study

Li,

Liu,

Jiang

et al. 2024

BMC Plant Biol

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“…Hilal et al (2000) tested fungi isolated from the roots and seeds of Luffa aegyptiaca Mill and found that M. phaseolina was not pathogenic to this crop. Subsequent studies also highlighted the resistance of this cucurbitaceous plant when inoculated with other root pathogens (Bruton et al, 2000;Kwon et al, 2001;Namisy et al, 2023;Bindal et al, 2023).…”

Section: Introductionmentioning

confidence: 98%

“…The lack of knowledge about the in uence of diseases transmitted by root pathogens has hindered the exploitation of this material for possible agricultural purposes (Bindal et al, 2023). Further research is therefore needed to better understand these effects and develop appropriate management strategies.…”

Section: Introductionmentioning

confidence: 99%

“…However, for the grafting technique to be effective, it is necessary to select plant material that is compatible with the target crop, easy to replicate, and provides resistance to these pathogens (Davis et al, 2008). According to Li et al (2016) and Bindal et al (2023), Luffa spp. seedlings can serve as rootstocks for other cucurbits such as pumpkin, cucumber, and melon.…”

Section: Introductionmentioning

confidence: 99%

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Reaction of Luffa spp. to Macrophomina pseudophaseolina inoculation

Viana,

Negreiros,

Silveira

et al. 2024

Preprint

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The genus Macrophomina is responsible for causing diseases in various species of the Cucurbitaceae family. This study aimed to evaluate the reaction of Luffa species when associated with M. pseudophaseolina. Initially, a prospection study was carried out to identify fungi associated with Luffa roots. Three isolates belonging to the M. pseudophaseolina species were identified and confirmed by DNA extraction and PCR amplification, using specific primers. One isolate was selected to test pathogenicity on Luffa. Twelve Luffa spp. accessions from the cucurbitaceous germplasm collection of Universidade Federal Rural do Semi-Árido were used, along with two positive controls: melon and watermelon plants. Inoculation was performed using the infested toothpick method. The plants were maintained under greenhouse conditions in a completely randomized design with four replications. The trial was repeated. The following variables were assessed: disease incidence and severity, reaction class, shoot length (SL), root length (RL), fresh shoot weight (FSW), dry shoot weight (DSW), fresh root weight (FRW), and dry root weight (DRW). Accession AB39 proved to be immune to the pathogen, as well as exhibiting the highest values of SL (163.9 cm), RL (43.4 cm), FSW (24.9 g), FRW (9.8 g), DSW (3.4 g), and DRW (0.5 g). These results indicate that the immune resistance of AB39 makes this accession promising, both in terms of disease resistance and vegetative growth, standing out as the best option among the accessions evaluated. Additionally, this is the first report of Luffa spp. acting as an alternative host of M. pseudophaseolina.

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Unveiling Clonostachys rosea bioactives: Investigating antifungal potential against Fusarium oxysporum through in vitro and in silico studies

Shravani,

Iruthayasamy,

Nallusamy

et al. 2024

Preprint

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Fusarium spp. pose significant threats to agriculture by causing wilt in various crops like ridge gourd. This study identifies Fusarium isolates from wilted ridge gourd plants and evaluates Clonostachys rosea's biocontrol potential. Twelve isolates were characterized using molecular and morphological analyses, revealing nine Fusarium oxysporum, two Fusarium brachygibbosum, and one Fusarium equiseti. Pathogenicity tests confirmed that F. oxysporum isolates were highly virulent, while other two species were weakly pathogenic. Antagonistic assays demonstrated that C. rosea significantly inhibited the growth of Fusarium spp. up to 71.59%. Microscopic analysis revealed its mycoparasitic activity, involving hyphal encirclement and degradation of Fusarium hyphae. Metabolite profiling through GC-MS characterized 44 bioactive compounds, including fatty acids, alcohols, amines, thiols, triazoles, and hydrazines. Molecular docking analysis revealed high binding affinities of these metabolites with key fungal proteins, suggesting potential mechanisms of antifungal action. C. rosea demonstrates potential as a biocontrol agent, with its antifungal metabolites providing new insights and strategies for managing Fusarium wilt in ridge gourd.

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Novel sources of resistance to fusarium wilt in Luffa species

Cited by 4 publications

References 39 publications

Analyzing genetic diversity in luffa and developing a Fusarium wilt-susceptible linked SNP marker through a single plant genome-wide association (sp-GWAS) study

Analyzing genetic diversity in luffa and developing a Fusarium wilt-susceptible linked SNP marker through a single plant genome-wide association (sp-GWAS) study

Reaction of Luffa spp. to Macrophomina pseudophaseolina inoculation

Unveiling Clonostachys rosea bioactives: Investigating antifungal potential against Fusarium oxysporum through in vitro and in silico studies

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