Novel splice isoforms of dairy goat DBC1 and their diverse mRNA expression profiles

Zhang, Xiaoyan; Li, Mingxun; Wu, Xianfeng; Pan, Chuanying; Lei, Chuzhao; Chen, Hong; Lan, Xianyong

doi:10.1016/j.smallrumres.2015.07.028

Cited by 5 publications

(4 citation statements)

References 47 publications

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“…The polymerase chain reactions (PCRs) were performed as described in our previous study (X. Y. Zhang et al, 2015).…”

Section: Identification Of Acox1 Alternative Splice Variantsmentioning

confidence: 99%

“…The RT-qPCR reaction system was implemented as described in our previous study (X. Y. Zhang et al, 2015). The steps in RT-qPCR were as follows: denaturation at 95 • C for 30 s, followed by 39 cycles of 15 s at 95 • C, and 50 s at 63 • C. The individual samples were amplified in triplicates, and a no-template control (NTC) was included (which contained ddH 2 O instead of cDNA template).…”

Section: Measurement Of Acox1 and Acox1-sv1 Mrna Levels Using Quantitmentioning

confidence: 99%

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Novel alternative splicing variants of <i>ACOX1</i> and their differential expression patterns in goats

Liu

ChengFang

et al. 2018

Arch. Anim. Breed.

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Abstract.As the first and rate-limiting enzyme of the peroxisomal β-oxidation pathway, acyl-coenzyme A oxidase 1 (ACOX1), which is regulated by peroxisome proliferator-activated alfa (PPARα), is vital for fatty acid oxidation and deposition, especially in the lipid metabolism of very long-chain fatty acids. Alternative splicing events of ACOX1 have been detected in rodents, Nile tilapia, zebra fish and humans but not in goats. Herein, we identified a novel splice variant of the ACOX1 gene, which was designated as ACOX1-SV1, in addition to the complete transcript, ACOX1, in goats. The length of the ACOX1-SV1 coding sequence was 1983 bp, which presented a novel exon 2 variation owing to alternative 5 -splice site selection in exon 2 and partial intron 1, compared to that in ACOX1. The protein sequence analysis indicated that ACOX1-SV1 was conserved across different species. Reverse-transcription quantitative real-time polymerase chain reaction (RT-qPCR) analysis showed that these two isoforms were expressed spatially and differently in different tissue types. ACOX1 and ACOX1-SV1 were expressed at high levels in liver, spleen, brain and adipose tissue in kid goats, and they were abundantly expressed in the fat, liver and spleen of adults. Interestingly, whether in kids or in adults, in fat, the mRNA level of ACOX1 was considerably higher than that of ACOX1-SV1. In contrast, in the liver, the expression of ACOX1-SV1 was considerably higher than that of ACOX1. This differential expression patterns showed the existence of a tissue-dependent splice regulation. These novel findings for ACOX1 should provide new insights for further studies on the function of ACOX1 and its variants that should aid in the breeding of goats with improved meat quality.

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“…The polymerase chain reactions (PCRs) were performed as described in our previous study (X. Y. Zhang et al, 2015).…”

Section: Identification Of Acox1 Alternative Splice Variantsmentioning

confidence: 99%

Section: Measurement Of Acox1 and Acox1-sv1 Mrna Levels Using Quantitmentioning

confidence: 99%

Novel alternative splicing variants of <i>ACOX1</i> and their differential expression patterns in goats

Liu

ChengFang

et al. 2018

Arch. Anim. Breed.

View full text Add to dashboard Cite

show abstract

“…Student t-test was to analyze independent samples, and data were expressed as the mean ± 1 standard deviation of duplicates. P-value b 0.05 (*) was considered statistically significant and highly significant Xie et al, 2014;Li et al, 2015;Zhang et al, 2015a;Zhang et al, 2015b).…”

Section: Transcript Expression Characteristics Analysismentioning

confidence: 99%

“…Different transcripts can be generated by alternative splicing (AS), which is a powerful and pluripotent regulatory mechanism (Saravanaperumal et al, 2014;Matlin et al, 2005;Zhang et al, 2015a;Zhang et al, 2015b). AS affects the gene expression and functional diversification of proteins (Lopez 1998).…”

Section: Introductionmentioning

confidence: 99%

Identification of novel alternative splicing transcript and expression analysis of bovine TMEM95 gene

Zhang

Cai

Yang

et al. 2016

Gene

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Exploration of dairy goat PITX2 alternative splice events and differential isoform expression

Zhang

Yang

et al. 2016

Small Ruminant Research

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Novel splice isoforms of dairy goat DBC1 and their diverse mRNA expression profiles

Cited by 5 publications

References 47 publications

Novel alternative splicing variants of <i>ACOX1</i> and their differential expression patterns in goats

Novel alternative splicing variants of <i>ACOX1</i> and their differential expression patterns in goats

Identification of novel alternative splicing transcript and expression analysis of bovine TMEM95 gene

Exploration of dairy goat PITX2 alternative splice events and differential isoform expression

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Novel splice isoforms of dairy goat DBC1 and their diverse mRNA expression profiles

Cited by 5 publications

References 47 publications

Novel alternative splicing variants of &lt;i&gt;ACOX1&lt;/i&gt; and their differential expression patterns in goats

Novel alternative splicing variants of &lt;i&gt;ACOX1&lt;/i&gt; and their differential expression patterns in goats

Identification of novel alternative splicing transcript and expression analysis of bovine TMEM95 gene

Exploration of dairy goat PITX2 alternative splice events and differential isoform expression

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Product

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Novel alternative splicing variants of <i>ACOX1</i> and their differential expression patterns in goats

Novel alternative splicing variants of <i>ACOX1</i> and their differential expression patterns in goats