Novel SPME fibers based on a plastic support for determination of plasma protein binding of thiosemicarbazone metal chelators: a case example of DpC, an anti-cancer drug that entered clinical trials

Benefits of Innovative and Fully Water-Compatible Stationary Phases of Thin-Film Microextraction (TFME) Blades

“…Additionally, the method is characterized by low energy consumption, low laboratory waste production, and device reusability, while still enabling performance comparable or superior to the traditional methods [ 5 ]. C 18 -coated SPME tips and fibers are already popular around the world, as indicated by the publications of many research groups within the last 2 years [ 6 , 7 , 8 , 9 ]. C 18 is also one of most popular coating types for the development of new SPME solutions, such as thin-film microextraction (TFME) blades.…”

Optimizing a High-Throughput Solid-Phase Microextraction System to Determine the Plasma Protein Binding of Drugs in Human Plasma

“…This latter advantage is due to the use of a matrix-compatible extraction phase in SPME, which eliminates the coextraction of macromolecules and allows the selective extraction of small molecules directly from complex matrices, such as plasma or blood. ,,− In addition to the matrix-compatible extraction phase, the support materials used in SPME devices (e.g., metals and plastic polymers) are also important in terms of clinical and medicinal applications. Of these support materials, plastics are particularly advantageous for use with complex biofluids, such as plasma and blood, due to their chemical robustness and smooth surfaces, which can minimize the attachment of background interferences, as has been reported in the literature. , …”

“…Given these advantages, this study utilizes plastic-supported BioSPME 96-pin devices for the high-throughput analysis of drug–protein binding in plasma underlying operating principles of solid-phase microextraction technology. The selected plastic-supported microsample prep device consists of 96 pins (Figure S1) coated with a small amount of matrix-compatible extraction phase, which facilitates the extraction of the small analytes of interest in the presence of macromolecules. , In addition, the use of minimal volumes of extraction phase enables the nondepletive extraction of analytes from small amounts of biofluids, and its plastic support structure provides an added advantage for its application in complex matrices such as plasma and blood. , Furthermore, the device’s 96-pin configuration enables direct sampling from 96-well plates and can be easily automated for high-throughput sampling. In the present study, various drugs with a wide range of polarities (logP) and protein binding properties (Table S2 and Table ) were selected as model analytes to investigate the performance and applicability of the BioSPME pin devices.…”

“…Of these support materials, plastics are particularly advantageous for use with complex biofluids, such as plasma and blood, due to their chemical robustness and smooth surfaces, which can minimize the attachment of background interferences, as has been reported in the literature. 35,36 Given these advantages, this study utilizes plastic-supported BioSPME 96-pin devices for the high-throughput analysis of drug−protein binding in plasma underlying operating principles of solid-phase microextraction technology. The selected plastic-supported microsample prep device consists of 96 pins (Figure S1) coated with a small amount of matrixcompatible extraction phase, which facilitates the extraction of the small analytes of interest in the presence of macromolecules.…”

“…32,33 In addition, the use of minimal volumes of extraction phase enables the nondepletive extraction of analytes from small amounts of biofluids, 22 and its plastic support structure provides an added advantage for its application in complex matrices such as plasma and blood. 35,36 Furthermore, the device's 96-pin configuration enables direct sampling from 96-well plates and can be easily automated for high-throughput sampling. In the present study, various drugs with a wide range of polarities (logP) and protein binding properties (Table S2 and Table 1) were selected as model analytes to investigate the performance and applicability of the BioSPME pin devices.…”