Novel strategy of cartilage repairing <i>via</i> application of <i>P. atlantica</i> with stem cells and collagen

Tanideh, Nader; Borzooeian, Giti; Lotfi, Mehrzad; Sani, Mahsa; Irajie, Cambyz; Ghaemmaghami, Parvin; Koohi-Hosseinabadi, Omid; Tanideh, Romina; Sadeghian, Mahdi Hashempour; Borzooeian, Zahra; Iraji, Aida

doi:10.1111/aor.14026

Cited by 3 publications

(3 citation statements)

References 86 publications

(167 reference statements)

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“…For the six marker genes previously identified, we found that CDKN1A, FOSB, STMN2, and SLC2A3 genes were reported to be associated with cartilage inflammation in OA (40)(41)(42)(43)(44)(45)(46)(47)(48)(49)(50)(51). For example, the expression level of CDKN1A will increase correspondingly during cartilage degeneration (44), IL-17 and IL-1beta induced collagenase-3 production through AP-1 occurred with differential protein complex, and stimulation resulted in FOSB activation (52), STMN2 has significant differences between preserved and subchondral bone (48).…”

Section: Discussionmentioning

confidence: 85%

Identification of SCRG1 as a Potential Therapeutic Target for Human Synovial Inflammation

Liu

Zhang

et al. 2022

Front. Immunol.

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Synovial inflammation of joint tissue is the most important cause of tissue damage, joint destruction, and disability and is associated with higher morbidity or mortality. Therefore, this study aims to identify key genes in osteoarthritis synovitis tissue to increase our understanding of the underlying mechanisms of osteoarthritis and identify new therapeutic targets. Five GEO datasets with a total of 41 normal synovial membrane tissues and 45 osteoarthritis synovial membrane samples were used for analysis, and seven common differential genes were identified. The classification model constructed by LASSO analysis showed that six genes including CDKN1A, FOSB, STMN2, SLC2A3, TAC, and SCRG1 can be used as biomarkers of osteoarthritis, and the SCRG1 gene shows importance in osteoarthritis. Furthermore, drug database enrichment found that these six DEGs may be the drug targets of synovitis in osteoarthritis, and Valproic Acid CTD 00006977 may be a potential targeted therapeutic drug of SCRG1. Spearman correlation analysis was performed on the SCRG1 gene, and 27 genes with consistent expression were obtained. Functional analysis showed that 27 genes were mainly involved in metabolism, complement, antigen presentation, apoptosis, and regulation of immune pathways. The co-regulatory network of TFs-miRNA suggested that the SCRG1 gene may be regulated by hsa-miR-363-3p miRNA. In conclusion, SCRG1, as a diagnostic marker of osteoarthritis, co-regulates immune-related pathways through the interaction of related proteins, playing an important role in the occurrence and development of osteoarthritis, which may be a novel drug target.

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Section: Discussionmentioning

confidence: 85%

Identification of SCRG1 as a Potential Therapeutic Target for Human Synovial Inflammation

Liu

Zhang

et al. 2022

Front. Immunol.

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“…Animals were obtained from the Laboratory Animal Center of Shiraz University of Medical Sciences. They were fed with a standard diet and water ad libitum [ 30 ]. In the normal condition, the daily rat food consumption was around 7 to 10 g. Rats were randomly divided into 5 groups (34 animals in each group) as follows: Group 1: control group (no induced wound) Group 2: wound + no intervention Group 3: wound + normal saline (W + N.S), 0.9% mouthwash, every day.…”

Section: Methodsmentioning

confidence: 99%

The effect of hydroalcoholic extract of Psidium guajava L. on experimentally induced oral mucosal wound in rat

Ghaderi

Ebrahimi

Aslani

et al. 2022

BMC Complement Med Ther

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Background The aim of this study was to evaluate the biological effects of hydroalcoholic extract of Psidium guajava L leaves and phenytoin as a standard agent on the induced oral mucosal wound. Methods Hundred seventy Sprague Dawley rats were grouped in 5 clusters randomly. Oral mucosal wounds were induced in all rats except for the control group. Phenytoin and guajava leaf extract were used as a mouthwash. Twelve rats from the 5 groups were euthanized on day 7th and 10th, and 10 rats from each group were sacrificed on the 14th day. Interleukin-6 and total antioxidant capacity were determined in the serum. The tissues were evaluated for pathological and stereological assessments. Phytochemical analyses were performed on the hydroalcoholic extract of Psidium guajava L to determine the antioxidant potency. Results Total phenolic content test and DPPH analysis demonstrated the high potential of antioxidant capacity of Psidium guajava L. Decreasing IL-6 and increasing TAC were seen in the guajava hydroalcoholic extract and phenytoin groups. The difference of IL-6 between the wound treated guajava group and the wounded group was significant. The wound treated guajava group and wound treated phenytoin group on the 14th day increased the number of fibroblast cells and volume density of sub-mucosae effectively to the same thickness to be considered as a healed sub-mucosae layer. The volume density of the epithelium changes showed statistically significant different responses based on gender. Conclusion In conclusion, hydroalcoholic extract of Psidium guajava L leaves might exert theraputic effects on oral mucositis.

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“…In the last days of research, blood samples were collected and centrifuged at 4000 rpm for 20 min; the serum cytokines including TNF- α (TNF-alpha assay kit, Diaclone, Besançon cedex, France), IL-6 (Interleukin-6 assay kit, ZellBio GmbH, Ulm, Germany), and NO (Nitric oxide assay kit, KNO-96, KiaZist, Bahar, Hamadan, Iran) were determined [ 44 – 46 ].…”

Section: Methodsmentioning

confidence: 99%

Anti-Inflammatory, Antioxidant, and Healing-Promoting Effects of Aloe vera Extract in the Experimental Colitis in Rats

Naini

Zargari-Samadnejad

Mehrvarz

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Background. Ulcerative colitis is a worldwide chronic gastrointestinal disease characterized by variable extensions of colon mucosal inflammation. The available drugs have an incomplete response with various side effects and socioeconomic impacts. Aloe barbadensis Miller (Aloe vera) is a well-known medicinal plant with diverse pharmacological and therapeutic activities. As a result, in the current study, Aloe vera was selected to evaluate its therapeutic effects on experimental colitis in rats. Methods. This study is intended to evaluate the possible beneficial effect of Aloe vera for the treatment of experimental colitis. Trinitrobenzenesulfonic acid (TNBS) was used to induce experimental colitis in 60 of 70 Wistar rats. The rats were grouped in 7 clusters including healthy control, negative, positive control (received sulfasalazine), and test groups treated with Aloe vera extracts via oral or rectal routes. Macroscopic and histologic factors as well as the biochemical parameters were evaluated on day 7. Results. In the present study, it was found that serum levels of tumor necrosis factor-α (75 vs. 44 pg./ml), interleukin-6 (41 vs. 21 pg/ml), and nitric oxide (24 vs. 6 μm/ml) in TNBS-induced untreated colitis treatment were significantly increased as compared to healthy control. Similar patterns were also observed in malondialdehyde (76.41 vs. 236.35 μg/mg) and myeloperoxidase (4.24 vs. 29.38 U/mg) in colonic tissue. Among different treatments, rectal administration of Aloe vera extract (400 mg/kg) exhibited the best result in which serum concentration of tumor necrosis factor-α (55 pg/ml), interleukin-6 (24 pg/ml), and nitric oxide (10 μm/ml) and the levels of malondialdehyde (102.67 μg/mg), as well as myeloperoxidase (12.29 U/mg) in colon tissue, were reduced as compared to the untreated group. Also, the body weight and colon weight/length ratios were more improved in the treated group with 400 mg/kg Aloe vera extract, rectally. Conclusion. Aloe vera extract exhibited a therapeutic effect in TNBS-induced colitis, and local, rectal administration of Aloe vera extract was more effective than oral administration.

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Novel strategy of cartilage repairing via application of P. atlantica with stem cells and collagen

Cited by 3 publications

References 86 publications

Identification of SCRG1 as a Potential Therapeutic Target for Human Synovial Inflammation

Identification of SCRG1 as a Potential Therapeutic Target for Human Synovial Inflammation

The effect of hydroalcoholic extract of Psidium guajava L. on experimentally induced oral mucosal wound in rat

Anti-Inflammatory, Antioxidant, and Healing-Promoting Effects of Aloe vera Extract in the Experimental Colitis in Rats

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