Novel Strategy to Accelerate Bone Regeneration of Calcium Phosphate Cement by Incorporating 3D Plotted Poly(lactic‐<i>co</i>‐glycolic acid) Network and Bioactive Wollastonite

Qian, Guowen; Fan, Peirong; He, Fupo; Ye, Jiandong

doi:10.1002/adhm.201801325

Cited by 34 publications

(20 citation statements)

References 37 publications

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“…The direct melt extrusion-based process has been shown to be suitable for 3D printing of aliphatic polyesters, and numerous studies have been done using these polymers for various tissue engineering applications. − Herein, we used a 3D Bioplotter (EnvisionTEC, Gladbeck, Germany); a direct extrusion-based printer that provides high resolution, the extruded fiber (single-strand) orientation can be precisely controlled at a resolution of up to 100 μm, and a low amount of polymer is needed in the printer before starting to fabricate scaffolds. Bioplotter has been used widely to print 3D scaffolds from aliphatic polyesters for both in vitro and in vivo characterizations. − The drawback when using degradable polymers with this printer is that there is no stirring, and the polymer is kept in the cartridge during the printing period, factors that influence the degradation. Melt-processed thermoplastics such as polylactide (PLA) and its copolymers normally include stabilizers or chain extenders to prevent degradation, but this is not an option when the manufactured device is intended for use in vivo. , Poly(ε-caprolactone) (PCL) is therefore the primary type used for medical-grade applications because of its low melting point. , When degradable polymers are used in 3D printing it is thus important to consider their degradability, since degradation causes complications during the 3D printing process and also a significant impact on the final properties of the polymer. ,− …”

Section: Introductionmentioning

confidence: 99%

Printability and Critical Insight into Polymer Properties during Direct-Extrusion Based 3D Printing of Medical Grade Polylactide and Copolyesters

et al. 2019

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Various 3D printing techniques currently use degradable polymers such as aliphatic polyesters to create well-defined scaffolds. Even though degradable polymers are influenced by the printing process, and this subsequently affects the mechanical properties and degradation profile, degradation of the polymer during the process is not often considered. Degradable scaffolds are today printed and cell–material interactions evaluated without considering the fact that the polymer change while printing the scaffold. Our methodology herein was to vary the printing parameters such as temperature, pressure, and speed to define the relationship between printability, polymer microstructure, composition, degradation profile during the process, and rheological behavior. We used high molecular weight medical-grade (co)polymers, poly(l-lactide-co-ε-caprolactone) (PCLA), poly(l-lactide-co-glycolide) (PLGA), and poly(d,l-lactide-co-glycolide) (PDLGA), with l-lactide content ranging from 25 to 100 mol %, for printing in an extrusion-based printer (3D Bioplotter). Optical microscopy confirmed that the polymers were printable at high resolution and good speed, until a certain degree of degradation. The results show also that printability can not be claimed just by optimizing printing parameters and highlight the importance of a careful analysis of how the polymer’s structure and properties vary during printing. The polymers thermally decomposed from the first processing minute and caused a decrease in the average block length of the lactide blocks in the copolymers and generated lower crystallinity. Poly(l-lactide) (PLLA) and PCLA are printable at a higher molecular weight, less degradation before printing was possible, compared to PLGA and PDLGA, a result explained by the higher complex viscosity and more elastic polymeric melt of the copolymer containing glycolide (GA) and lactide (LA). In more detail, copolymers comprised of LA and ε-caprolactone (CL) formed lower molecular weight compounds over the course of printing, while the PLGA copolymer was more susceptible to intermolecular transesterification reactions, which do not affect the overall molecular weight, but cause changes in the copolymer microstructure. This results in a longer printing time for PLGA than PLLA and PCLA.

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Section: Introductionmentioning

confidence: 99%

Printability and Critical Insight into Polymer Properties during Direct-Extrusion Based 3D Printing of Medical Grade Polylactide and Copolyesters

et al. 2019

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“…Besides common calcium phosphate‐based ceramics, researchers have been trying to use wollastonite from various sources and synthesis as a biomaterial (de Lima et al, ; Fiocco et al, ; Palakurthy, VGR, Samudrala, & AA, ). This calcium silicate reacts fast in simulated body fluid (Núñez‐Rodríguez, Encinas‐Romero, Gómez‐Álvarez, Valenzuela‐García, & Tiburcio‐Munive, ) and the response in vivo demonstrates that osteoblasts migrate to the surface of wollastonite and colonized the surface at the contact areas with the cortical regions and also bone marrow (de Aza, Luklinska, Martinez, Anseau, & Guitian, ; Qian, Fan, He, & Ye, ).…”

Section: Introductionmentioning

confidence: 99%

Synthesis and in vivo evaluation of a scaffold containing wollastonite/β‐TCP for bone repair in a rabbit tibial defect model

et al. 2019

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Scaffolds are models designed to aid the interaction between cells and extracellular bone matrix, providing structural support for newly formed bone tissue. In this work, wollastonite with β‐TCP porous ceramic scaffolds was developed by the polymer sponge replication. Their microstructure, cell viability and bioactivity were tested. in vivo was performed to evaluate the use of a calcium silicate‐based implant in the repair of rabbit tibias. Holes were made in the both proximal and distal tibial metaphysis of each animal and filled with calcium silicate‐based implant, and in the left tibia, no implant were used, serving as control group. Animals underwent euthanasia after 30 and 60 days of study. The animals were submitted to clinical‐radiographic evaluations and their histology was analyzed by optical and scanning electron microscope. The studied calcium silicate implant provided biocompatibility and promoted bone formation, stimulating the process of bone repair in rabbits, features observed by gradual radiopacity shown in the radiographic evaluations.

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“…Biodegradable polymer materials have tough structures while bioceramics improve the electrical conductivity of bone, thus allowing for flexible adjustment of its composition and microstructure while maintaining its respective advantages (Puppi et al, 2012;Dos Santos et al, 2019;Alksne et al, 2020;Zhu et al, 2020). Qian et al (2019) infiltrated pastes containing calcium phosphate bone cement (CPC) and wollastonite (WS) into a 3D plotted PLGA network to fabricate plastic CPC-based composite cement (PLGA/WS/CPC) for the first time. The PLGA/WS/CPC recovered the plasticity of CPC after being heated above the glass transition temperature of PLGA (Figure 7).…”

Section: Synthetic Polymersmentioning

confidence: 99%

Recent Trends in the Development of Bone Regenerative Biomaterials

Tang

Liu

et al. 2021

Front. Cell Dev. Biol.

130

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The goal of a biomaterial is to support the bone tissue regeneration process at the defect site and eventually degrade in situ and get replaced with the newly generated bone tissue. Biomaterials that enhance bone regeneration have a wealth of potential clinical applications from the treatment of non-union fractures to spinal fusion. The use of bone regenerative biomaterials from bioceramics and polymeric components to support bone cell and tissue growth is a longstanding area of interest. Recently, various forms of bone repair materials such as hydrogel, nanofiber scaffolds, and 3D printing composite scaffolds are emerging. Current challenges include the engineering of biomaterials that can match both the mechanical and biological context of bone tissue matrix and support the vascularization of large tissue constructs. Biomaterials with new levels of biofunctionality that attempt to recreate nanoscale topographical, biofactor, and gene delivery cues from the extracellular environment are emerging as interesting candidate bone regenerative biomaterials. This review has been sculptured around a case-by-case basis of current research that is being undertaken in the field of bone regeneration engineering. We will highlight the current progress in the development of physicochemical properties and applications of bone defect repair materials and their perspectives in bone regeneration.

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Novel Strategy to Accelerate Bone Regeneration of Calcium Phosphate Cement by Incorporating 3D Plotted Poly(lactic‐co‐glycolic acid) Network and Bioactive Wollastonite

Cited by 34 publications

References 37 publications

Printability and Critical Insight into Polymer Properties during Direct-Extrusion Based 3D Printing of Medical Grade Polylactide and Copolyesters

Printability and Critical Insight into Polymer Properties during Direct-Extrusion Based 3D Printing of Medical Grade Polylactide and Copolyesters

Synthesis and in vivo evaluation of a scaffold containing wollastonite/β‐TCP for bone repair in a rabbit tibial defect model

Recent Trends in the Development of Bone Regenerative Biomaterials

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