Novel strontium-doped bioactive glass nanoparticles enhance proliferation and osteogenic differentiation of human bone marrow stromal cells

Abstract

The present study investigates a new family of bioactive glass nanoparticles with and without Sr-doping focusing on the influence of the nanoparticles on human bone marrow stromal cells (hBMSCs) in vitro. The bioactive glass nanoparticles were fabricated by flame spray synthesis and a particle diameter of 30-35 nm was achieved. Glass nanoparticles were undoped (BG 13-93-0Sr) or doped with 5 wt% strontium (Sr) ) and used at concentrations of 10 and 100 g/cm² (particles per culture plate area), respectively. Cells were cultured for 14 days after which the samples were analysed regarding metabolic activity and expression of various bone-specific genes. Cell growth and morphology indicated the high cytocompatibility of the nanoparticulate bioactive glass. The presence of the nanoparticles enhanced cell growth compared to the plain polystyrene control group. At a concentration of 100 g/cm², Sr-doped particles led to significantly enhanced gene expression of osteocalcin, collagen type 1 and vascular endothelial growth factor. Thus, Sr-doped nanoparticles showing a dose-dependent increase of osteogenic differentiation in hBMSCs are a promising biomaterial for bone regeneration purposes.

The osteogenic response of mesenchymal stromal cells to strontium-substituted bioactive glasses

“…More recently, Strobel et al . () reported the increased expression of Bglap , Col1a1 and Runx2 in human MSCs after 14 days of exposure to cell culture medium containing nanoparticles of strontium‐substituted bioactive glass fabricated by flame spray synthesis. However, the bioactive glass content was regularly renewed throughout the experiment, as opposed to what was done in this study.…”

“…() in the study of mesoporous bioactive glass, by Strobel et al . () in the study of bioactive glass nanoparticles fabricated by flame spray synthesis, and by Zhu et al . () in the study of strontium‐substituted borate bioactive glass compositions.…”

Soy protein isolate/bioactive glass composite membranes: Processing and properties

“…The compositions of the nBG were very close to i) 45S5 composition (47.8 wt% SiO2, 25.1 wt% CaO, 22.6 wt% Na2O, 4.6 wt% P2O5) [20], ii) 13-93 BG (53 wt.% SiO2, 20 wt.% CaO, 6 wt.% Na2O, 4 wt.% P2O5, 12 wt.% K2O, 5 wt.% MgO ) and iii) 13-93-5Sr (mean particle size 30-35 nm) composition (53 wt.% SiO2, 15 wt.% CaO, 6 wt.% Na2O, 4 wt.% P2O5, 12 wt.% K2O, 5 wt.% MgO, 5 wt.% SrO). These glass nano powders were fabricated by the flame spray method [20,21].…”

Biomedical applications of natural-based polymers combined with bioactive glass nanoparticles

“…In this regard, Strobel et al assessed the osteogenic potential of BGNPs containing strontium (SiO 2 -CaO-P 2 O 5 -Na 2 O-K 2 O-MgO-SrO) on human bone marrow stromal cells (hBMSCs). 80 Cell growth and morphology studies showed the high cytocompatibility of the nanoparticulate bioactive glass. Moreover, the strontium-doped particles improved the expression of osteocalcin, collagen type I and vascular endothelial growth factor.…”

“…Moreover, the strontium-doped particles improved the expression of osteocalcin, collagen type I and vascular endothelial growth factor. 80 However, is also important to notice that increasing their strontium content can delay the bioactive behavior of BGNPs because of the inhibitory effect of strontium on apatite mineralization. 81 Xue et al synthesized monodispersed europium (Eu)-doped BGNPs with a dual function of bioactivity and luminescence by a template-assisted sol-gel method.…”