Novel substrate specificity of d-arabinose isomerase from Klebsiella pneumoniae and its application to production of d-altrose from d-psicose

Polyol Conversion Specificity ofBacillus pallidus

“…D-Arabinose isomerase was active on L-fucose, D-arabinose, L-altrose, L-galactose, L-xylose, and L-glucose. 3,11) The enzyme from B. pallidus showed almost the same substrate specificity, i.e., Darabinose, D-altrose, L-galactose, L-xylose, and L-glucose, as was found in previous studies (Fig. 3).…”

“…[1][2][3][4][5] In addition, bioconversion strategies for all rare sugars have been schematized. 6) Biotransformation is generally recognized as environmentally friendly compared to other chemical processes, and it is economically competitive in terms of cost and productivity.…”

“…3). 3,11) The benefits of using B. pallidus biotransformation in the mass production of rare sugars from polyols are low cost, and the fact that the complete reaction can be carried out without addition of a coenzyme. By the synergistic effect of a resting cell reaction of isomerase and polyol dehydrogenase, we can improve the production yield by increasing the initial concentration of polyol, because the conversion reactions of polyol and isomerization are developed at the same time and the concentration of intermediate ketose is maintained in the reaction mixture.…”

Enzymes for the biocatalytic production of rare sugars

“…In contrast, very little activity could be detected on the mirror image substrates L-lyxose and L-mannose [54]. Many more isomerases have been shown to be active on sugars similar to their wild-type substrates [2,12,57,71,85,86,88,94,117].…”

Cloning, Expression, and Transcription Analysis ofL-Arabinose Isomerase Gene fromMycobacterium smegmatisSMDU

“…Recently, rare sugars increased attraction not only in the food industry but also in pharmaceutical and nutritional industries on account of multipurpose applications such as their use as reduced-calorie sweeteners, inhibitors of microbial growth, bulking agents, antioxidants, and nucleoside analogues. [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16] We are working mainly on the production of various kinds of rare sugars using microorganisms screened from a variety of natural habitats and their enzymes. Using these microorganisms, we are studying several aldose-ketose isomerases (ketol isomerases), such as Lfucose isomerase (D-arabinose isomerase), L-arabinose isomerase (L-AI), L-ribose isomerase, and L-rhamnose isomerase, which are applicable in the production of various rare sugars as well as D-tagatose 3-epimerase and polyol dehydrogenases.…”