Dietary requirement and fitness of phytophagous insect pest depend upon the nutrient chemistry of host plant. The present research was carried out to investigate the association of host-plant-preference and mortality of Phenacoccus solenopsis first instar influenced by chemical characteristics of plant species. The experiment was carried out at (25 + 5ºC and 60 + 5% RH) in Integrated Pest Management Laboratory, University of Agriculture, Faisalabad, Pakistan during 2012. First instar exhibited maximum (attractiveness index = 0.75) toward Gossypium hirsutum followed by Trianthema portulacastrum (0.34) as compared Hibiscus rosa-chinensis (1.00) kept as standard. Mortality percentage of 1 st instar of CMB among 25 tested plant species ranged from 10% in cotton to 80% in Conyza bonariensis. Cluster analysis for biochemical traits showed that cluster-1 comprised of Lantana camara, H.
rosa-sinensis, Helianthus annuus, Parthenium hysterophorus, Withania somnifera, Euphorbia prostrate, Portulaca oleracea, Solamum melongena, T. portulacastrum, G. hirsutum, Abelmoschus esculentus andCapsicum frutescens that had maximum attractiveness and less mortality. Cluster-2 Convolvulus arvensis, Eclipta prostrate, Amaranthus spinosus, Clerodendron inerme and Tribulus terrestris had medium whereas cluster-3 Launea nudicaulis, Chinopodium morale, C. album, Achyranthes aspera, C. bonariensis and Digera arvensis least attractiveness index but highest mortality. Principal component analysis (PCA) depicted that first three PCs expressed 83% of the total variability. Mortality of pest was negatively correlated with nitrogen and but positively with potassium, phosphorus, sodium, reducing sugar and total sugar. In conclusion, chemical contents affected attractiveness index and mortality of P. solenopsis; however there is need to explore allelochemicals imparting resistance against P. solenopsis.