Novel svVEGF isoforms from Macrovipera lebetina venom interact with neuropilins

Aloui, Zohra; Hoos, Sylviane; Geretti, Elena; Kharmachi, Habib; Haumont, Pierre Yves; Mejdoub, Hafedh; Klagsbrun, Michael; England, Patrick; Gasmi, Ammar

doi:10.1016/j.bbrc.2009.08.068

Cited by 7 publications

(6 citation statements)

References 28 publications

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“…Subsequently, anion exchange chromatography was employed to purify the proteins based on their charge. The reported isoelectric points (pIs) of svVEGFs range from 7.8 to 8.7, as documented in previous studies [26,27,31,42]. In our investigation, at a pH of 7.8, CdtVEGF demonstrated a moderate binding capacity to the anionic resin employed in the exchange chromatography, leading to its elution at an intermediate point along the gradient.…”

Section: Discussionsupporting

confidence: 82%

“…Concerning the structural characterization of Cdt VEGF, we performed mass spectrometry analysis using digestion with trypsin, given that a substantial number of svVEGFs possess their amino terminal residue blocked by pyroglutamic acid [ 28 , 36 , 42 ].…”

Section: Discussionmentioning

confidence: 99%

“…The induction of vascular permeability from Cdt VEGF corroborates reports of other svVEGFs that also led to an increase when tested in animal models at low concentrations (10 ng/mL, 0.5 nM) [ 18 , 26 , 31 , 57 , 64 ]. In another study, svVEGF IC2 from M. lebetina snake venom, when tested on C57Bl/6 mice, induced higher vascular permeability than when tested on Swiss lineage [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

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Beyond Angiogenesis: The Multitasking Approach of the First PEGylated Vascular Endothelial Growth Factor (CdtVEGF) from Brazilian Rattlesnake Venom

Ferreira,

Oliveira,

Bordon

et al. 2023

Toxins

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A pioneering study regarding the isolation, biochemical evaluation, functional assays and first PEGylation report of a novel vascular endothelial growth factor from Crotalus durissus terrificus venom (CdtVEGF and PEG-CdtVEGF). CdtVEGF was isolated from crude venom using two different chromatographic steps, representing 2% of soluble venom proteins. Its primary sequence was determined using mass spectrometry analysis, and the molecule demonstrated no affinity to heparin. The Brazilian crotalid antivenom recognized CdtVEGF. Both native and PEGylated CdtVEGF were able to induce new vessel formation and migration, and to increase the metabolic activity of human umbilical endothelial vascular cells (HUVEC), resulting in better wound closure (~50% within 12 h) using the native form. CdtVEGF induced leukocyte recruitment to the peritoneal cavity in mice, with a predominance of neutrophil influx followed by lymphocytes, demonstrating the ability to activate the immune system. The molecule also induced a dose-dependent increase in vascular permeability, and PEG-CdtVEGF showed less in vivo inflammatory activity than CdtVEGF. By unraveling the intricate properties of minor components of snake venom like svVEGF, this study illuminates the indispensable significance of exploring these molecular tools to unveil physiological and pathological processes, elucidates the mechanisms of snakebite envenomings, and could possibly be used to design a therapeutic drug.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Beyond Angiogenesis: The Multitasking Approach of the First PEGylated Vascular Endothelial Growth Factor (CdtVEGF) from Brazilian Rattlesnake Venom

Ferreira,

Oliveira,

Bordon

et al. 2023

Toxins

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“…Several VEGFs isolated from the venom of Macrovipera lebetina have been identified as preferentially binding the VEGF receptor [ 90 , 91 ]. Isolated VEGFs have also been shown to bind to VEGF co-receptors, NRP1 and NRP2.…”

Section: Macrovipera Lebetinamentioning

confidence: 99%

“…Isolated VEGFs have also been shown to bind to VEGF co-receptors, NRP1 and NRP2. Interestingly, the binding affinity and selectivity differs between the different VEGFs for distinct NRPs, rendering the molecules interesting therapeutic candidates [ 91 ].…”

Section: Macrovipera Lebetinamentioning

confidence: 99%

Vipers of the Middle East: A Rich Source of Bioactive Molecules

et al. 2018

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Snake venom serves as a tool of defense against threat and helps in prey digestion. It consists of a mixture of enzymes, such as phospholipase A2, metalloproteases, and l-amino acid oxidase, and toxins, including neurotoxins and cytotoxins. Beside their toxicity, venom components possess many pharmacological effects and have been used to design drugs and as biomarkers of diseases. Viperidae is one family of venomous snakes that is found nearly worldwide. However, three main vipers exist in the Middle Eastern region: Montivipera bornmuelleri, Macrovipera lebetina, and Vipera (Daboia) palaestinae. The venoms of these vipers have been the subject of many studies and are considered as a promising source of bioactive molecules. In this review, we present an overview of these three vipers, with a special focus on their venom composition as well as their biological activities, and we discuss further frameworks for the exploration of each venom.

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