Novel synthesis of super peroxidase mimetic polycrystalline mixed metal hexacyanoferrates nanoparticles dispersion

Abstract

Mixed metal-hexacyanoferrates (MHCF) have shown promising applications for technological development particularly in energy storage technologies, ferromagnetic materials, and electrode materials. However, the challenge of controlling their chemical synthesis, solubility, polycrystallinity and nanogeometry have restricted the practical implementation of MHCF in commercial designs. Further, MHCFs have shown potential as peroxidase mimetics. There is still challenging demand to enhance the catalytic efficiency of such materials to the equivalent of biocatalytic activity thereby allowing the precise control of the mimetic character and ultimately leading to the development of new materials as powerful peroxidases i.e. Horseradish peroxidase (HRP) replacement during enzyme and immuno-sensors development. We report herein a new process that allows the controlled synthesis of biocompatible mixed MHCF nanoparticles with a variety of transition metal ions. The resulting polycrystalline mixed MHCF shows enhanced catalytic efficiency to hydrogen peroxide (H2O2) as compared to that of polycrystalline Prussian blue (PB) made under similar conditions with significant increase in catalytic efficiency as a function of respective hetero-transition metal ion of mixed FeHCF in the order of FeHCF < Mn-FeHCF < Ni-FeHCF < Cu-FeHCF justifying as perfect peroxidase replacement.

3-Aminopropyltrimethoxysilane and 3-Glycidoxypropyltrimethoxysilane Mediated Synthesis of Graphene and its Nanocomposite: Potential Bioanalytical Appliactions

“…We have recently reported the synthesis of such functional PBNPs involving the active role of 3-APTMS [13,14]. Such process also allow the synthesis of mixed metal hexacyanoferrate with nearly all combinations of hetero-transition metals [14,15]. Indeed interesting findings on the interaction of PBNPs and graphene has been observed and discussed vide infra.…”

“…In addition to that some of such functional alkoxysilanes like 3-aminopropyltrimethoxysilane (3-APTMS) and 3-glycidoxypropyltrimethoxysilane (3-GPTMS)) have recently been utilized as reagents and stabilizer during the synthesis of noble metal nanoparticles under ambient [10][11][12]. Further the active role of 3-APTMS has also been demonstrated during the synthesis of prussian blue nanoparticles (PBNPs) [13,14]. Such reducing and stabilizing ability of 3-GPTMS and 3-APTMS has directed to use the same during the synthesis of graphene from graphene oxide.…”

“…The use of such 3-APTMS and 3-GPTMS may allow the formation of functional graphene that may possess activity for making nanocomposite with known catalytic material displaying unusual property combinations and unique design possibilities as reported earlier [13,14]. Usually graphene based nanocomposites have been troubled with small specific surface area and low conductivity as well as poor durability, which are ascribed to the inevitable aggregation between individual graphene sheets driven by strong π-π interaction.…”

“…Prussian blue naoparticles (PBNPs) are known catalytic material as artificial peroxidase and the functional compatibility of the same with as prepared graphene may enforce the nanocomposite formation imparting exceptional catalytic properties. We have recently reported the synthesis of such functional PBNPs involving the active role of 3-APTMS [13,14]. Such process also allow the synthesis of mixed metal hexacyanoferrate with nearly all combinations of hetero-transition metals [14,15].…”

“…The potential application of as synthesized graphene and its nanocomposite with PBNPs as peroxidase mimetic has been examined. Since the PBNPs display remarkable electrocatalytic behavior during hydrogen peroxide sensing [13,14], the electrochemistry of as prepared graphene-PBNPs nanocomposite has also been studied. Accordingly, present investigation has been attempted to investigate the following: (1) functionalized alkoxysilane mediated controlled synthesis of graphene and its nanocomposite; (2) peroxidase mimetic activity of graphene and its nanocomposite for H 2 O 2 detection; (3) electrocatalytic activity of same for H 2 O 2 reduction.…”