Novel technique for scaling up of micropropagated Ruta graveolens shoots using liquid culture systems: A step towards commercialization

Diwan, Renuka; Malpathak, Nutan

doi:10.1016/j.nbt.2008.02.002

Cited by 25 publications

(30 citation statements)

References 13 publications

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“…That could clarify the highest content of FUC and rutin of R. gravoelens on medium supplemented with the lowest N concentration; however, the C/N ratio altered the production. R. gravoelens shoots grown on MS medium had 16.64 mg g -1 DW of FUC (Diwan and Malpathak 2008) which is higher than the maximum production (11.4 mg g -1 ) on our study using medium enriched with high P concentration; however, Ekiert et al (2001) achieved similar content of FUC. Also, increasing N content on the growth medium higher that of MS reduced the biosynthesis of FUC and rutin.…”

Section: Resultscontrasting

confidence: 77%

“…These results could emphasize the hypothesis of George and de Klerk (2008) which suggested that growth can continue for a short while after P depletion; hence, elevating its concentration on the growth medium up to 3.71 mM is recommended in order to increase the rate of shoot multiplication. Diwan and Malpathak (2008) observed good regeneration efficiency from R. gravoelens nodal explants of field-grown source on stander MS medium. These explants might have a higher concentration of P than seedlings.…”

Section: Resultsmentioning

confidence: 70%

“…Results showed that higher sucrose/N ratio improved the percentage of DW especially at the lowest concentration of N. The effect of C/N ratio on shoot morphogenesis and growth has been suggested by (Bhojwani and Razdan 1996). These results are more promising for R. gravoelens micropropagation using seedling explants than those of Diwan and Malpathak (2008) who obtained only 7.21 g of shoot FW with a GI 6.21 after 4 weeks of maintaining 2-3 g of shoots clumps in MS medium with 30 sucrose and 4.44 lM BA and NAA. Values are means ± SD * On the same column means followed by different letters are significantly different at p \ 0.05 (Tukey's multiple range test)…”

Section: Resultsmentioning

confidence: 84%

“…The culture weight on individual flasks was calculated during the growth period following the equation (culture weight = W2 -W1 -W4). The GI was calculated as described by Diwan and Malpathak (2008), then the shoots were air dried to estimate the DW, FUC and rutin content.…”

Section: Organogenesismentioning

confidence: 99%

“…Several studies have been investigated the effect of explant type and plant growth regulators on R. gravoelens micropropagation and the in vitro production of secondary products (De Castro and De Barros 1999;Ekiert et al 2001;Bohidar et al 2008;Diwan and Malpathak 2007;Renuka and Nutan 2010). Diwan and Malpathak (2008) subcultured R. gravoelens shoots in liquid culture and reported that spermine and putrescine improved the production of (FUC) from 11.6 to 23.6 mg g -1 of air-dried shoots). After 4 weeks of maintaining 2 g of shoots clumps in the growth medium they obtained 51.2 shoots which weighted 7.21 g with a growth index (GI) 6.21%.…”

Section: Introductionmentioning

confidence: 99%

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In vitro mass production of Ruta gravoelens L. for secondary products production

Mohamed

Ibrahim

2011

Acta Physiol Plant

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Hypocotyls explants of 1-week-old Ruta gravoelens L. seedlings showed high regeneration ability on Murashige and Skoog's medium supplemented with 5.2 mM H 2 PO 4 -, 250 mM adenine sulphate, 3.0% sucrose and 8.87 lM BA. Increasing phosphate and adenine sulphate concentrations had pronounced effects on organogenesis and shoot growth compared to the stander MS medium. Multiple shoots were successfully maintained for 60 days in static liquid medium without any hyperhydricity phenomena. Uncountable shoot buds and shoots were developed on the leaf surface which was in contact with the medium. The growth index increased linearly during the culture course (R 2 = 0.992-0.999) and the highest GI (30.43) was achieved on medium enriched with 90 mM nitrogen and 4.5% sucrose. Medium supplemented with 6.0% sucrose and 60 mM N yielded the highest biomass (4.37 g per flask). However, medium with the stander N content and 6.0% sucrose developed the maximum percentage of biomass (6.67%). The highest percentage of furanocoumarins (11.46 mg g -1 dry weight) calculated as xanthotoxin was produced in medium enriched with 90 mM N and 4.5 or 6.0% sucrose, whereas the maximum rutin (73.5 mg g -1 dry weight) was on medium supplemented with 90 mM N and 4.5% sucrose. Developing system seems to be used for secondary metabolites production because the shoot rooting did not appear. So, all energy is devoted for shoots multiplication, their growth and secondary metabolites production.

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Section: Resultscontrasting

confidence: 77%

Section: Resultsmentioning

confidence: 70%

Section: Resultsmentioning

confidence: 84%

Section: Organogenesismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In vitro mass production of Ruta gravoelens L. for secondary products production

Mohamed

Ibrahim

2011

Acta Physiol Plant

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Establishment, Culture, and Scale-up of Brugmansia candida Hairy Roots for the Production of Tropane Alkaloids

Cardillo

Talou

Giulietti

2016

Methods in Molecular Biology

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Brugmansia candida (syn. Datura candida) is a South American native plant that produces tropane alkaloids. Hyoscyamine, 6β-hydroxyhyoscyamine (anisodamine), and scopolamine are the most important ones due to their anticholinergic activity. These bioactive compounds have been historically and widely applied in medicine and their demand is continuous. Their chemical synthesis is costly and complex, and thereby, these alkaloids are industrially produced from natural producer plants. The production of these secondary metabolites by plant in vitro cultures such as hairy roots presents certain advantages over the natural source and chemical synthesis. It is well known that hairy roots produced by Agrobacterium rhizogenes infection are fast-growing cultures, genetically stable and able to grow in hormone-free media. Additionally, recent progress achieved in the scaling up of hairy root cultures makes this technology an attractive tool for industrial processes. This chapter is focused on the methods for the induction and establishment of B. candida hairy roots. In addition, the scaling up of hairy root cultures in bioreactors and tropane alkaloid analysis is discussed.

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Bioreactor-Grown Shoot Cultures for the Secondary Metabolite Production

Krol¹,

Kokotkiewicz²,

Szopa³

et al. 2020

Reference Series in Phytochemistry

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Novel technique for scaling up of micropropagated Ruta graveolens shoots using liquid culture systems: A step towards commercialization

Cited by 25 publications

References 13 publications

In vitro mass production of Ruta gravoelens L. for secondary products production

In vitro mass production of Ruta gravoelens L. for secondary products production

Establishment, Culture, and Scale-up of Brugmansia candida Hairy Roots for the Production of Tropane Alkaloids

Bioreactor-Grown Shoot Cultures for the Secondary Metabolite Production

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