Novel technique for the direct flow cytofluorometric analysis of human basophils in unseparated blood and bone marrow, and the characterization of phenotype and peroxidase of human basophils

Eosinophils develop in distinct stages and are recruited to peripheral sites by alternatively activated macrophages

“…Further, expression of the IL-4R␣-chain on splenic eosinophils was determined by flow cytometry. In contrast to CD4 T cells and non-CD4, noneosinophil cells, which expressed IL-4R␣ on the cell surface, no staining was observed on eosinophils, a finding consistent with previous observations using human cells [25] (Fig. 7C).…”

G-CSF suppresses allergic pulmonary inflammation, downmodulating cytokine, chemokine and eosinophil production

“…G-CSF increased neutrophils in blood and neutropoiesis in culture, as expected from expression of its receptor in the neutrophil lineage (Toba et al 1999). However, since eosinophils lack G-CSF receptors (Toba et al 1999), and G-CSF does not stimulate eosinophil colony formation (Enokihara et al 1988) by itself, an indirect mechanism is likely to account for its effects.…”

“…However, since eosinophils lack G-CSF receptors (Toba et al 1999), and G-CSF does not stimulate eosinophil colony formation (Enokihara et al 1988) by itself, an indirect mechanism is likely to account for its effects. Although G-CSF might indirectly affect the eosinophil lineage by favoring neutrophils in competition for critical resources in a restricted bone-marrow environment, shared by these closely related lineages (Iwasaki et al 2005), the same considerations would apply to mononuclear phagocytes, which were unaffected by G-CSF in this study.…”

Toll-like receptor 2 ligands activate human basophils for both IgE-dependent and IgE-independent secretion

“…The most credible explanation for this finding is that these cells do not normally express the coreceptor, CD14. 23 Unlike TLR2, the signaling mediated through TLR4 is very much dependent on coexpression of CD14 and its accessory protein (MD2), which complex with TLR4, allowing LPS signaling to occur in cells expressing these components. 24 By not expressing CD14, basophils probably never recognize LPS for subsequent signaling through TLR4.…”