Novel thin layer flow-cell screen-printed graphene electrode for enzymatic sensors

Kanso, Hussein; García, María Begoña González; Llano, Laura F.l; Ma, Su; Ludwig, Roland; Fanjul‐Bolado, Pablo; Santos, David Hernández

doi:10.1016/j.bios.2016.08.069

Cited by 25 publications

(12 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This configuration brings important advantages since it simplifies operability and effectiveness of working in FIA systems. A similar format was already reported in two previous works [16,17] where enzymatic amperometric based sensors were developed. However, this is the first time that these electrodes, integrated in the necessary accessories, were described for ECL applications.…”

Section: A Miniaturized Flow Injection Analysis System For Electrogenmentioning

confidence: 86%

A Miniaturized Flow Injection Analysis System for Electrogenerated Chemiluminescence−Based Assays

et al. 2017

View full text Add to dashboard Cite

A new miniaturized flow injection analysis (FIA) system for developing electrochemiluminescence (ECL)-based assays is presented. Novel thin-layer flowÀcell (TLFCL) screen-printed carbon electrodes integrated in one-channel flow cells were used as the electrode surface. These TLFCL electrodes, combined with miniaturized electrochemical and optical instrumentation, allowed a high degree of automation and simplicity of the assay. The performance of this high-throughput flow system was corroborated with a proof-of-concept centered on H 2 O 2 detection. For that purpose, an ECL transduction chemistry mechanism based on a electrochemiluminescence resonance energy transfer (ECL-RET) donorÀacceptor strategy, between an electro-oxidized luminophore (luminol) to a fluorophore (fluorescein), in the presence of the analyte of interest, was carried out. The results showed that fluorescein ECL-RET luminescent response increases the sensitivity of H 2 O 2 detection by up to one order of magnitude, in comparison with a luminolÀH 2 O 2 fluorescein-free assay. All experiments were performed in a decentralized, small-sized, easy-to-use FIA system coupled to ECL detection instrumentation.The light emitted from electrogenerated chemiluminescence (ECL) is a process in which electrochemically generated species combine to undergo electron-transfer reactions to form excited, light-emitting species, giving place to the development of high sensitive and selective assays. [1][2][3][4][5][6][7][8][9][10][11] In order to increase the automation of luminescent analytical procedures, the detection of ECL response by flow injection analysis system (FIA) is an interesting option. [12,13] Flow analysis is characterized by highly reproducible and throughput sampling. In addition, sequential injection analysis allows a considerable reduction in the consumption of the sample and reagents. [14] Bearing in mind that ECL based advances are moving towards the development of portable, high-throughput and point-of-care testing systems, [8,15] disposable screen-printed electrodes coupled to a small-sized, non-conventional, FIA system appears as an attractive possibility.For that purpose, in this work, screen-printed electrodes integrated in one channel flow-cell (TLFCL, Thin layer flow-cell) were used as the electrode surface. In these electrodes a transparent slide, that usefully allows the detection of air bubbles inside the cell, is attached over the screen-printed electrodes platform delimiting a flow channel. The injection of sample volume is done through an "in-line luer" injection port, placed closest to the electrochemical cell, where the injection can be easily controlled by operator through a precise syringe. This configuration brings important advantages since it simplifies operability and effectiveness of working in FIA systems. A similar format was already reported in two previous works [16,17] where enzymatic amperometric based sensors were developed. However, this is the first time that these electrodes, integrated in the necessary accessori...

show abstract

Section: A Miniaturized Flow Injection Analysis System For Electrogenmentioning

confidence: 86%

A Miniaturized Flow Injection Analysis System for Electrogenerated Chemiluminescence−Based Assays

et al. 2017

View full text Add to dashboard Cite

show abstract

“…In 2017, nine new FIA-SPE methods for the determination of the following analytes were developed: isoniazid drug, [51] serotonin, [52] uric acid, [53] nitrite, [54] proton activity, [55] H 2 O 2 , [56] lactose, [57] 2-nitro-p-phenylenediamine, [58] and the electroactivity of nanoparticles. [59] During this year, two new flow cells were proposed for coupling with SPEs.…”

Section: Spes Coupled To Fia Systemsmentioning

confidence: 99%

“…The second methodology utilized a thin layer flow-cell for SPEs used as enzymatic sensors. [57] The main objective was the development of a cell, which can be less aggressive than the currently utilized wall-jet flow cells towards a biological recognition element immobilized upon the surface of the electrode. In this report, the SPE was integrated into an one-channel disposable thin-layer transparent flow-cell, offering the advantages of miniaturization, facile electrode modification and replacement.…”

Section: Spes Coupled To Fia Systemsmentioning

confidence: 99%

“…Platinized AuSPE NR NR [55] Hydrogen peroxide NR SPCE 1.0 18 [56] Lactose Milk Different types of SPEs modified with corynascus thermophilus 180 12 [57]…”

Section: Uric Acidmentioning

confidence: 99%

“…According to the authors, the new analytical FIA system is a sustainable easy‐to‐use device solving analytical problems in which small sample consumption, simplicity and portability are demanded. The second methodology utilized a thin layer flow‐cell for SPEs used as enzymatic sensors . The main objective was the development of a cell, which can be less aggressive than the currently utilized wall‐jet flow cells towards a biological recognition element immobilized upon the surface of the electrode.…”

Section: Spes Coupled To Fia Systemsmentioning

confidence: 99%

See 2 more Smart Citations

An Overview of Recent Electroanalytical Applications Utilizing Screen‐Printed Electrodes Within Flow Systems

et al. 2020

View full text Add to dashboard Cite

This review addresses the use of screen-printed electrodes (SPEs) coupled to flow systems such as flow injection analysis (FIA), batch injection analysis (BIA), and high-performance liquid chromatography (HPLC) systems over the last six years (since 2014). The combination of SPEs and flow systems is a powerful synergy to increase the throughput of measurements, improve electrode lifetime, and reduce reagent consumption and waste generation. Recently, commercial flow cells for SPEs were made available by different companies and potential new users that are unable to construct homemade electrochemical flow cells have been attracted to work in the area. This overview aims to show both trends and future potential for the development of new methods useful for modern electroanalysis and/or portable applications.

show abstract

Recent Biosensing Applications of Graphene‐Based Nanomaterials

Arora

2019

Handbook of Graphene

View full text Add to dashboard Cite

Novel thin layer flow-cell screen-printed graphene electrode for enzymatic sensors

Cited by 25 publications

References 47 publications

A Miniaturized Flow Injection Analysis System for Electrogenerated Chemiluminescence−Based Assays

A Miniaturized Flow Injection Analysis System for Electrogenerated Chemiluminescence−Based Assays

An Overview of Recent Electroanalytical Applications Utilizing Screen‐Printed Electrodes Within Flow Systems

Recent Biosensing Applications of Graphene‐Based Nanomaterials

Contact Info

Product

Resources

About