Novel Tn916-like elements confer aminoglycoside/macrolide co-resistance in clinical isolates of Streptococcus gallolyticus ssp. gallolyticus

Kambarev, Stanimir; Pecorari, Frédéric; Corvec, Stéphane

doi:10.1093/jac/dky016

Cited by 10 publications

(8 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Macrolide antibiotics are the oldest and most successful clinical ribosomal targeted antibiotics which target bacterial ribosomes to inhibit protein synthesis [43]. The macrolide antibiotics we detected were consistent with a study that proved clinical isolates of S. gallolyticus harbored macrolide [10]. Moreover, the speci c genes didn't play any role in the antibiotic resistance of S. gallolyticus strains (Fig.…”

Section: Identi Cation Of Virulence Factors For S Gallolyticus Strain...supporting

confidence: 86%

“…These genes affect the host immune system and participate in adhesion to the lining of the cardiac epithelium, leading to infection and endocarditis [9]. Moreover, S. gallolyticus has been identi ed as a putative laterally active reservoir for these antibiotic resistance determining factors in the mammalian gastrointestinal microbiota, in particular, bacteria a liated to Firmicutes, such as Enterococci and Eubacteria [10]. For example, a previous study has reported that the S. gallolyticus subsp strains isolated from the samples of the clinical harbor a large number of antibiotic resistance genes that are susceptible to erythromycin and tetracycline [11].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparative analysis of 31 Streptococcus gallolyticus strains uncovers their potential risks from the perspectives of virulence factors and antibiotic resistance genes

Zhang

Bai

Wang

et al. 2022

Preprint

View full text Add to dashboard Cite

Streptococcus gallolyticus is an opportunistic pathogen and plays important role in various ecological niches, particularly in the intestinal tract of mammals. Obtaining the composition of S. gallolyticus strains from multiple perspectives is beneficial to broadening the knowledge of S. gallolyticus. Hence, we collected the genomic datasets of 31 S. gallolyticus strains and conducted the pan-genome analysis to systemically illustrate the genetic features and investigate the mechanism of its pathogenicity. Our results showed that the pan-genome of S. gallolyticus is composed of 4,606 homologous clusters and presented an open pan-genome structure. The phylogenetic analysis revealed the complicated relationship among S. gallolyticus strains. Six CAZyme families were identified from 182 orthologous genes that mainly derived from the core genome to clarify the carbohydrate metabolism of S. gallolyticus strains. The results showed that the metabolic ability of carbohydrates of 31 S. gallolyticus strains was different and these strains preferred glycosides and a crucial influence on the formation and modification of glycans and glycoconjugates. Particularly, the results of virulence factors indicated that the pathogenicity of S. gallolyticus strains was related to immunity, bile acid metabolism, and membrane synthesis. Besides, to guide the clinical treatment, we investigated the composition of antibiotic resistance genes of S. gallolyticus strains and claimed that these strains are resistant to antibiotics. Overall, our work systematically explores the genetic background of S. gallolyticus, provides an in-depth understanding of the biological characteristics of S. gallolyticus, and sheds light on the clinical detection and prevention of S. gallolyticus.

show abstract

Section: Identi Cation Of Virulence Factors For S Gallolyticus Strain...supporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Comparative analysis of 31 Streptococcus gallolyticus strains uncovers their potential risks from the perspectives of virulence factors and antibiotic resistance genes

Zhang

Bai

Wang

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Moreover, a recent molecular characterization of 39 foodborne tetracycline-resistant isolates of S. bovis identified a novel mosaic tet (S/M) fusion [72]. Indeed, a new mechanism has been recently identified associated with macrolide resistance: two previously unknown conjugative transposons, Tn6263 and Tn6331, were found to confer aminoglycoside/macrolide co-resistance identified in a clinical isolate of SGSG responsible for infective endocarditis and colorectal cancer [73].…”

Section: Antimicrobial Resistancementioning

confidence: 99%

An Overview on Streptococcus bovis/Streptococcus equinus Complex Isolates: Identification to the Species/Subspecies Level and Antibiotic Resistance

Pompilio

Bonaventura

Gherardi

2019

IJMS

View full text Add to dashboard Cite

Streptococcus bovis/Streptococcus equinus complex (SBSEC), a non-enterococcal group D Streptococcus spp. complex, has been described as commensal bacteria in humans and animals, with a fecal carriage rate in humans varying from 5% to over 60%. Among streptococci, SBSEC isolates represent the most antibiotic-resistant species—with variable resistance rates reported for clindamycin, erythromycin, tetracycline, and levofloxacin—and might act as a reservoir of multiple acquired genes. Moreover, reduced susceptibility to penicillin and vancomycin associated with mobile genetic elements have also been detected, although rarely. Since the association of SBSEC bacteremia and colon lesions, infective endocarditis and hepatobiliary diseases has been established, particularly in elderly individuals, an accurate identification of SBSEC isolates to the species and subspecies level, as well as the evaluation of antibiotic resistance, are needed. In this paper, we reviewed the major methods used to identify SBSEC isolates and the antimicrobial resistance rates reported in the scientific literature among SBSEC species.

show abstract

“…A better understanding of the dissemination mechanism of AR genes is essential for monitoring and controlling drug resistance. Previous studies have revealed that mobile genetic elements (MGEs) play significant roles in the horizontal transfer of AR genes in Streptococcus ( Puopolo et al, 2007 ; Chancey et al, 2015 ; Davies et al, 2015 ; Kambarev et al, 2018 ). Integrative conjugative elements (ICEs), prophages and tandem ICE-prophages located at different insertion sites were common MGEs described in S. suis ( Uruen et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of integrative and conjugative elements carrying antibiotic resistance genes of Streptococcus suis isolated in China

Wang

Bai

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

Streptococcus suis, an emerging zoonotic pathogen, is important reservoirs of antibiotic resistance genes that play critical roles in the horizontal transfer of corresponding resistances. In the present study, 656 antibiotic resistance (AR) genes were detected in 154 of 155 genomes of S. suis strains isolated from the nasopharynx of slaughtered pigs and the lungs of diseased pigs in China. The AR genes were clustered into 11 categories, consisting of tetracycline, macrolides, lincosamide, streptogramin, aminoglycoside, trimethoprim, amphenicols, nucleoside, quinupristin/dalfopristin, glycopeptide, and oxazolidinones resistance genes. In order to investigate the transmission patterns of the AR genes, AR genes-associated the mobile genetic elements (MGEs) were extracted and investigated. Twenty ICEs, one defective ICE, one tandem ICE, and ten prophages were found, which mainly carried tetracycline, macrolides/lincosamides/streptogramin (MLS), and aminoglycosides resistance genes. Three types of DNA cargo with AR genes were integrated into specific sites of ICEs: integrative mobilizable elements (IMEs), cis-IMEs (CIMEs), and transposon Tn916. Obvious differences in AR gene categories were found among the three cargo types. IMEs mainly harbored tetracycline and MLS resistance genes. CIMEs mainly carried aminoglycoside resistance genes, while transposon Tn916 carried only the tet (M) gene. Nearly all AR genes in ICEs were carried by IMEs and CIMEs. IMEs were prevalent and were also detected in additional 29 S. suis genomes. The horizontal transfer of IMEs and CIMEs may play critical role in ICE evolution and AR gene transmission in the S. suis population. Our findings provide novel insights into the transmission patterns of AR genes and the evolutionary mechanisms of ICEs in S. suis.

show abstract

Novel Tn916-like elements confer aminoglycoside/macrolide co-resistance in clinical isolates of Streptococcus gallolyticus ssp. gallolyticus

Cited by 10 publications

References 21 publications

Comparative analysis of 31 Streptococcus gallolyticus strains uncovers their potential risks from the perspectives of virulence factors and antibiotic resistance genes

Comparative analysis of 31 Streptococcus gallolyticus strains uncovers their potential risks from the perspectives of virulence factors and antibiotic resistance genes

An Overview on Streptococcus bovis/Streptococcus equinus Complex Isolates: Identification to the Species/Subspecies Level and Antibiotic Resistance

Characterization of integrative and conjugative elements carrying antibiotic resistance genes of Streptococcus suis isolated in China

Contact Info

Product

Resources

About